EIF4G1 (NM_198244) Human Untagged Clone

CAT#: SC309370

EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3



  "NM_198244" in other vectors (4)

CNY 10,450.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-EIF4G1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_198244, the custom clone sequence may differ by one or more nucleotides
ATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGA
CAGGGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCA
GGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCA
GCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTTTGATGAACCAG
CCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCAA
GGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACC
CCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCT
GTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTG
CCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCA
CCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGG
GACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACT
GGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTA
GAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCT
CCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCT
GAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCC
TCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCC
CCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAG
GTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCA
GCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAA
GCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAG
AGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTG
GCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTT
GGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAG
CCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCT
GAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATC
CAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAG
GAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATG
CAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACA
CCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACT
CCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGG
CCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGA
CCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTG
AACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGA
GGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATC
CTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCC
ATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCA
GAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTG
CCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGT
CAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAG
ATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCT
CGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAA
CTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCAT
GATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGAC
TTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATTAAA
GAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGG
AGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAG
GAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGC
AGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTG
GATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCA
CGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCT
GGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGAC
GCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAA
CAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGC
CGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGA
GGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGC
AAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCA
GCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTA
CCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAG
GCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAG
GAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTG
GAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCT
GGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAG
GACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATT
CTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGA
CCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATG
GGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTA
CCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGA
GAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTG
GAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAAC
CTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGC
TATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGA
GCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCC
CTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGAC
GCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAG
GACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAG
TGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA
Restriction Sites Please inquire     
ACCN NM_198244
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198244.1, NP_937887.1
RefSeq Size 5225 bp
RefSeq ORF 4539 bp
Locus ID 1981
Protein Pathways Viral myocarditis
Gene Summary The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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