COL11A1 (NM_080629) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CO11A1; COLL6; DFNA37; STL2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309482 representing NM_080629. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCGTGGTCCTCTAGGTGGAAAACGAAACGGTGGCTCTGGGATTTCACCGTAACAACCCTCGCA TTGACCTTCCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATGTACTAAAAGCACTAGAT TTTCACAATTCTCCAGAGGGAATATCAAAAACAACGGGATTTTGCACAAACAGAAAGAATTCTAAAGGC TCAGATACTGCTTACAGAGTTTCAAAGCAAGCACAACTCAGTGCCCCAACAAAACAGTTATTTCCAGGT GGAACTTTCCCAGAAGACTTTTCAATACTATTTACAGTAAAACCAAAAAAAGGAATTCAGTCTTTCCTT TTATCTATATATAATGAGCATGGTATTCAGCAAATTGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTG TTTGAAGACCACACTGGAAAACCTGCCCCAGAAGACTATCCCCTCTTCAGAACTGTTAACATCGCTGAC GGGAAGTGGCATCGGGTAGCAATCAGCGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAG AAAACCACGAAACCACTTGATAGAAGTGAGAGAGCAATTGTTGATACCAATGGAATCACGGTTTTTGGA ACAAGGATTTTGGATGAAGAAGTTTTTGAGGGGGACATTCAGCAGTTTTTGATCACAGGTGATCCCAAG GCAGCATATGACTACTGTGAGCATTATAGTCCAGACTGTGACTCTTCAGCACCCAAGGCTGCTCAAGCT CAGGAACCTCAGATAGATGAGAAAAAGAAATCCAATTTCAAAAAGAAGATGAGGACAGTGGCTACTAAA TCAAAGGAAAAATCCAAAAAGTTTACACCCCCCAAATCTGAAAAATTTTCATCCAAGAAGAAGAAAAGT TATCAAGCATCAGCAAAAGCCAAACTAGGGGTAAAGGCAAACATCGTTGATGATTTTCAAGAATACAAC TATGGAACAATGGAAAGTTACCAGACAGAAGCTCCTAGGCATGTTTCTGGGACAAATGAGCCAAATCCA GTTGAAGAAATATTTACTGAAGAATATCTAACGGGAGAGGATTATGATTCCCAGAGGAAAAATTCTGAG GATACACTATATGAAAACAAAGAAATAGACGGCAGGGATTCTGATCTTCTGGTAGATGGAGATTTAGGC GAATATGATTTTTATGAATATAAAGAATATGAAGATAAACCAACAAGCCCCCCTAATGAAGAATTTGGT CCAGGTGTACCAGCAGAAACTGATATTACAGAAACAAGCATAAATGGCCATGGTGCATATGGAGAGAAA GGACAGAAAGGAGAACCAGCAGTGGTTGAGCCTGGTATGCTTGTCGAAGGACCACCAGGACCAGCAGGA CCTGCAGGTATTATGGGTCCTCCAGGTCTACAAGGCCCCACTGGACCCCCTGGTGACCCTGGCGATAGG GGCCCCCCAGGACGTCCTGGCTTACCAGGGGCTGATGGTCTACCTGGTCCTCCTGGTACTATGTTGATG TTACCGTTCCGTTATGGTGGTGATGGTTCCAAAGGACCAACCATCTCTGCTCAGGAAGCTCAGGCTCAA GCTATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGCCCACCTGGCCCAATGGGTCTAACTGGAAGACCA GGTCCTGTGGGGGGGCCTGGTTCATCTGGGGCCAAAGGTGAGAGTGGTGATCCAGGTCCTCAGGGCCCT CGAGGCGTCCAGGGTCCCCCTGGTCCAACGGGAAAACCTGGAAAAAGGGGTCGTCCAGGTGCAGATGGA GGAAGAGGAATGCCAGGAGAACCTGGGGCAAAGGGAGATCGAGGGTTTGATGGACTTCCGGGTCTGCCA GGTGACAAAGGTCACAGGGGTGAACGAGGTCCTCAAGGTCCTCCAGGTCCTCCTGGTGATGATGGAATG AGGGGAGAAGATGGAGAAATTGGACCAAGAGGTCTTCCAGGTGAAGCTGGCCCACGAGGTTTGCTGGGT CCAAGGGGAACTCCAGGAGCTCCAGGGCAGCCTGGTATGGCAGGTGTAGATGGCCCCCCAGGACCAAAA GGGAACATGGGTCCCCAAGGGGAGCCTGGGCCTCCAGGTCAACAAGGGAATCCAGGACCTCAGGGTCTT CCTGGTCCACAAGGTCCAATTGGTCCTCCTGGTGAAAAAGGACCACAAGGAAAACCAGGACTTGCTGGA CTTCCTGGTGCTGATGGGCCTCCTGGTCATCCTGGGAAAGAAGGCCAGTCTGGAGAAAAGGGGGCTCTG GGTCCCCCTGGTCCACAAGGTCCTATTGGATACCCGGGCCCCCGGGGAGTAAAGGGAGCAGATGGTGTC AGAGGTCTCAAGGGATCTAAAGGTGAAAAGGGTGAAGATGGTTTTCCAGGATTCAAAGGTGACATGGGT CTAAAAGGTGACAGAGGAGAAGTTGGTCAAATTGGCCCAAGAGGGGAAGATGGCCCTGAAGGACCCAAA GGTCGAGCAGGCCCAACTGGAGACCCAGGTCCTTCAGGTCAAGCAGGAGAAAAGGGAAAACTTGGAGTT CCAGGATTACCAGGATATCCAGGAAGACAAGGTCCAAAGGGTTCCACTGGATTCCCTGGGTTTCCAGGT GCCAATGGAGAGAAAGGTGCACGGGGAGTAGCTGGCAAACCAGGCCCTCGGGGTCAGCGTGGTCCAACG GGTCCTCGAGGTTCAAGAGGTGCAAGAGGTCCCACTGGGAAACCTGGGCCAAAGGGCACTTCAGGTGGC GATGGCCCTCCTGGCCCTCCAGGTGAAAGAGGTCCTCAAGGACCTCAGGGTCCAGTTGGATTCCCTGGA CCAAAAGGCCCTCCTGGACCACCTGGGAAGGATGGGCTGCCAGGACACCCTGGGCAACGTGGGGAGACT GGATTTCAAGGCAAGACCGGCCCTCCTGGGCCAGGGGGAGTGGTTGGACCACAGGGACCAACCGGTGAG ACTGGTCCAATAGGGGAACGTGGGCATCCTGGCCCTCCTGGCCCTCCTGGTGAGCAAGGTCTTCCTGGT GCTGCAGGAAAAGAAGGTGCAAAGGGTGATCCAGGTCCTCAAGGTATCTCAGGGAAAGATGGACCAGCA GGATTACGTGGTTTCCCAGGGGAAAGAGGTCTTCCTGGAGCTCAGGGTGCACCTGGACTGAAAGGAGGG GAAGGTCCCCAGGGCCCACCAGGTCCAGTTGGCTCACCAGGAGAACGTGGGTCAGCAGGTACAGCTGGC CCAATTGGTTTACCAGGGCGCCCGGGACCTCAGGGTCCTCCTGGTCCAGCTGGAGAGAAAGGTGCTCCT GGAGAAAAAGGTCCCCAAGGGCCTGCAGGGAGAGATGGAGTTCAAGGTCCTGTTGGTCTCCCAGGGCCA GCTGGTCCTGCCGGCTCCCCTGGGGAAGACGGAGACAAGGGTGAAATTGGTGAGCCGGGACAAAAAGGC AGCAAGGGTGACAAGGGAGAAAATGGCCCTCCCGGTCCCCCAGGTCTTCAAGGACCAGTTGGTGCCCCT GGAATTGCTGGAGGTGATGGTGAACCAGGTCCTAGAGGACAGCAGGGGATGTTTGGGCAAAAAGGTGAT GAGGGTGCCAGAGGCTTCCCTGGACCTCCTGGTCCAATAGGTCTTCAGGGTCTGCCAGGCCCACCTGGT GAAAAAGGTGAAAATGGGGATGTTGGTCCCATGGGGCCACCTGGTCCTCCAGGCCCAAGAGGCCCTCAA GGTCCCAATGGAGCTGATGGACCACAAGGACCCCCAGGGTCTGTTGGTTCAGTTGGTGGTGTTGGAGAA AAGGGTGAACCTGGAGAAGCAGGGAACCCAGGGCCTCCTGGGGAAGCAGGTGTAGGCGGTCCCAAAGGA GAAAGAGGAGAGAAAGGGGAAGCTGGTCCACCTGGAGCTGCTGGACCTCCAGGTGCCAAGGGGCCACCA GGTGATGATGGCCCTAAGGGTAACCCGGGTCCTGTTGGTTTTCCTGGAGATCCTGGTCCTCCTGGGGAA CCTGGCCCTGCAGGTCAAGATGGTGTTGGTGGTGACAAGGGTGAAGATGGAGATCCTGGTCAACCGGGT CCTCCTGGCCCATCTGGTGAGGCTGGCCCACCAGGTCCTCCTGGAAAACGAGGTCCTCCTGGAGCTGCA GGTGCAGAGGGAAGACAAGGTGAAAAAGGTGCTAAGGGGGAAGCAGGTGCAGAAGGTCCTCCTGGAAAA ACCGGCCCAGTCGGTCCTCAGGGACCTGCAGGAAAGCCTGGTCCAGAAGGTCTTCGGGGCATCCCTGGT CCTGTGGGAGAACAAGGTCTCCCTGGAGCTGCAGGCCAAGATGGACCACCTGGTCCTATGGGACCTCCT GGCTTACCTGGTCTCAAAGGTGACCCTGGCTCCAAGGGTGAAAAGGGACATCCTGGTTTAATTGGCCTG ATTGGTCCTCCAGGAGAACAAGGGGAAAAAGGTGACCGAGGGCTCCCTGGAACTCAAGGATCTCCAGGA GCAAAAGGGGATGGGGGAATTCCTGGTCCTGCTGGTCCCTTAGGTCCACCTGGTCCTCCAGGTTTACCA GGTCCTCAAGGCCCAAAGGGTAACAAAGGCTCTACTGGACCCGCTGGCCAGAAAGGTGACAGTGGTCTT CCAGGGCCTCCTGGGTCTCCAGGTCCACCTGGTGAAGTCATTCAGCCTTTACCAATCTTGTCCTCCAAA AAAACGAGAAGACATACTGAAGGCATGCAAGCAGATGCAGATGATAATATTCTTGATTACTCGGATGGA ATGGAAGAAATATTTGGTTCCCTCAATTCCCTGAAACAAGACATTGAGCATATGAAATTTCCAATGGGT ACTCAGACCAATCCAGCCCGAACTTGTAAAGACCTGCAACTCAGCCATCCTGACTTCCCAGATGGTGAA TATTGGATTGATCCTAACCAAGGTTGCTCAGGAGATTCCTTCAAAGTTTACTGTAATTTCACATCTGGT GGTGAGACTTGCATTTATCCAGACAAAAAATCTGAGGGAGTAAGAATTTCATCATGGCCAAAGGAGAAA CCAGGAAGTTGGTTTAGTGAATTTAAGAGGGGAAAACTGCTTTCATACTTAGATGTTGAAGGAAATTCC ATCAATATGGTGCAAATGACATTCCTGAAACTTCTGACTGCCTCTGCTCGGCAAAATTTCACCTACCAC TGTCATCAGTCAGCAGCCTGGTATGATGTGTCATCAGGAAGTTATGACAAAGCACTTCGCTTCCTGGGA TCAAATGATGAGGAGATGTCCTATGACAATAATCCTTTTATCAAAACACTGTATGATGGTTGTGCGTCC AGAAAAGGCTATGAAAAGACTGTCATTGAAATCAATACACCAAAAATTGATCAAGTACCTATTGTTGAT GTCATGATCAATGACTTTGGTGATCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTCTT GGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_080629
Insert Size	5457 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080629.2
RefSeq Size	7327 bp
RefSeq ORF	5457 bp
Locus ID	1301
UniProt ID	P12107
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	182.4 kDa
Gene Summary	This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009] Transcript Variant: This variant (B) utilizes alternate exon 6, designated exon 6B, and encodes the longest isoform (B). There are no publicly available transcripts representing this variant; it is supported by mRNA annotation on DNA accession AF101112.1, protein AAF04726.1, submitted by the authors of PMID:10486316. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

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