IFT172 (NM_015662) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BBS20; NPHP17; osm-1; RP71; SLB; SRTD10; wim
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309828 representing NM_015662. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCAAAGGTGACCTGCATGGCT TGGTCCCAGAACAATGCCAAATTTGCTGTCTGCACAGTGGACCGAGTGGTCTTGCTGTATGATGAACAT GGAGAACGGAGAGATAAATTCTCCACCAAACCAGCTGACATGAAGTATGGCAGGAAGAGCTATATGGTG AAGGGCATGGCTTTTTCTCCTGATTCCACTAAAATTGCCATAGGACAGACTGACAACATCATCTATGTC TACAAGATTGGAGAAGATTGGGGTGACAAGAAAGTCATCTGCAACAAGTTCATCCAGACGAGTGCTGTC ACTTGTCTGCAATGGCCGGCAGAATACATCATTGTCTTTGGACTGGCTGAAGGGAAGGTTCGTTTAGCA AACACCAAAACTAATAAATCATCTACCATCTATGGGACAGAGTCTTACGTGGTGTCCCTGACAACAAAT TGCTCTGGGAAAGGAATTCTCTCTGGTCATGCAGATGGTACCATCGTTAGGTATTTCTTTGATGATGAA GGCTCTGGAGAGTCACAGGGGAAGTTGGTTAACCACCCGTGTCCACCCTATGCCTTGGCATGGGCAACC AATAGCATCGTGGCTGCAGGCTGTGATCGGAAAATTGTAGCCTATGGAAAAGAAGGTCACATGCTACAA ACTTTTGATTATAGCCGTGACCCTCAGGAGCGGGAGTTCACCACAGCTGTATCAAGTCCTGGGGGCCAG TCTGTTGTGCTAGGAAGTTATGACAGGCTTCGGGTGTTCAACTGGATCCCTCGAAGAAGCATCTGGGAA GAGGCAAAGCCCAAGGAGATTACCAATTTATACACCATCACTGCCTTGGCCTGGAAGCGGGATGGCTCA CGGCTCTGTGTGGGCACACTATGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGTATTTAC AAGAACAAGTTTGAGTTGACGTATGTGGGACCTAGCCAGGTGATTGTGAAGAACCTGTCATCAGGAACC CGAGTGGTGCTCAAGTCACACTATGGCTATGAGGTGGAAGAGGTGAAAATCCTAGGAAAGGAACGTTAC TTGGTGGCTCACACATCAGAAACACTGCTGCTGGGGGACCTGAACACTAATCGGCTTAGTGAGATAGCC TGGCAAGGATCTGGTGGCAATGAGAAGTATTTCTTTGAAAATGAGAATGTATGCATGATCTTCAATGCC GGAGAGCTAACCCTGGTGGAATATGGGAATAATGACACCCTGGGTTCTGTACGCACTGAATTCATGAAC CCCCACCTCATCAGTGTTCGTATTAATGAGAGGTGTCAGCGAGGAACAGAAGATAATAAGAAATTGGCT TATCTTATTGATATTAAGACTATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGCACCGTCAGC CATGAGAGCCGTGTGGATTGGCTGGAACTTAATGAGACTGGACACAAGCTCCTCTTCAGGGACCGGAAA CTTCGTTTGCATCTGTATGATATTGAAAGCTGCTCTAAGACAATGATCCTCAACTTCTGCTCCTATATG CAGTGGGTCCCAGGAAGTGACGTGCTGGTAGCTCAGAACCGAAACAGTCTGTGTGTATGGTACAACATT GAGGCACCTGAGAGAGTCACCATGTTCACTATTAGGGGTGATGTTATAGGTCTGGAGCGGGGCGGGGGA AAGACCGAGGTGATGGTGATGGAAGGTGTGACTACTGTTGCCTACACATTGGATGAGGGCCTCATCGAG TTTGGAACAGCCATTGATGATGGCAACTACATCCGGGCAACAGCCTTCTTAGAGACTCTGGAAATGACC CCAGAAACAGAGGCAATGTGGAAAACCTTGAGTAAACTGGCACTAGAGGCAAGGCAACTACACATTGCG GAGAGGTGCTTTTCTGCTTTGGGCCAAGTAGCAAAAGCTCGATTCCTGCATGAGACCAATGAGATTGCA GATCAAGTATCCCGGGAATATGGCGGAGAAGGAACAGACTTTTATCAGGTCCGAGCACGTCTAGCCATG CTGGAAAAGAACTACAAACTGGCTGAAATGATCTTTTTGGAACAGAATGCTGTGGAGGAGGCCATGGGC ATGTACCAGGAGCTACACCGTTGGGATGAGTGTATCGCTGTGGCTGAAGCCAAGGGGCACCCAGCCCTG GAGAAGCTACGTCGTAGTTACTACCAGTGGCTGATGGACACACAGCAAGAGGAGCGAGCAGGTGAACTA CAGGAGAGCCAAGGGGATGGGCTAGCAGCCATCAGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCT GCTCGGCTGGTGCTGACCCGAGAGGAACTGCTAGCCAACACAGAGCTGGTAGAACACATCACTGCAGCC CTTATCAAGGGGGAACTCTACGAAAGGGCAGGTGATCTCTTTGAGAAGATTCACAATCCACAGAAGGCC CTGGAGTGCTACCGTAAAGGCAACGCATTCATGAAAGCGGTAGAGCTGGCTCGATTGGCCTTCCCAGTG GAGGTGGTGAAACTAGAGGAGGCATGGGGGGACCACCTGGTGCAGCAGAAGCAGCTTGATGCAGCCATT AATCACTACATCGAAGCCAGGTGCTCCATTAAGGCAATTGAGGCCGCCCTGGGTGCCCGCCAGTGGAAG AAGGCAATTTATATATTAGATCTACAGGACCGGAACACTGCATCCAAATACTATCCTCTCGTGGCCCAA CACTATGCATCCCTGCAGGAGTATGAGATTGCTGAGGAGCTCTATACTAAGGGAGATCGGACAAAAGAT GCCATAGACATGTACACCCAGGCTGGTCGTTGGGAACAAGCCCACAAGCTGGCGATGAAATGCATGAGA CCAGAAGATGTGTCAGTGCTATACATCACTCAGGCCCAGGAAATGGAGAAGCAGGGCAAGTACCGTGAG GCTGAAAGGCTATATGTGACAGTACAAGAGCCTGATCTTGCCATCACCATGTACAAAAAGCACAAGTTG TATGATGACATGATCCGCCTGGTAGGGAAGCACCATCCAGATCTCCTCAGTGATACACACCTACATCTG GGCAAGGAGCTGGAGGCTGAAGGCCGACTACAGGAGGCTGAGTACCACTACCTCGAGGCCCAGGAATGG AAGGCAACAGTGAACATGTACCGGGCCAGTGGGCTTTGGGAAGAGGCCTACAGGGTGGCCAGAACTCAA GGAGGGGCTAATGCCCACAAACACGTGGCCTATCTGTGGGCAAAGAGCCTGGGAGGAGAGGCTGCAGTT AGACTGCTTAATAAGCTGGGACTCCTGGAAGCTGCTGTTGACCACGCTGCAGACAATTGCTCCTTTGAA TTTGCGTTTGAACTCTCTCGGCTGGCCCTCAAGCACAAAACCCCCGAGGTTCATCTCAAATATGCTATG TTCCTGGAGGATGAGGGTAAATTCGAAGAGGCTGAAGCTGAATTCATCAGAGCTGGTAAACCCAAGGAG GCAGTCCTCATGTTTGTCCATAACCAGGATTGGGAGGCAGCTCAGCGTGTGGCTGAGGCTCACGACCCT GACAGTGTCGCCGAGGTGCTTGTGGGACAGGCCCGGGGGGCCTTGGAGGAGAAGGACTTTCAGAAAGCA GAAGGGCTGCTGCTCCGGGCCCAGAGACCAGGCCTGGCCCTCAATTATTATAAGGAGGCTGGATTATGG AGTGACGCTCTGCGCATCTGCAAGGACTATGTGCCCAGCCAGCTGGAGGCTCTGCAGGAAGAATATGAG CGGGAAGCTACTAAGAAGGGGGCCAGGGGTGTGGAGGGATTTGTGGAACAAGCTCGACACTGGGAGCAG GCTGGAGAGTACAGCCGTGCCGTGGACTGCTACCTCAAAGTGCGAGACTCTGGAAACAGCGGCCTGGCG GAGAAGTGCTGGATGAAGGCAGCTGAACTCTCCATCAAGTTTCTGCCTCCCCAACGTAATATGGAAGTC GTTCTGGCTGTAGGACCCCAGCTGATTGGAATTGGAAAGCACAGTGCAGCTGCAGAGCTCTATCTGAAT CTGGACCTTGTCAAGGAAGCAATCGATGCTTTCATCGAGGGTGAGGAGTGGAACAAGGCGAAGCGTGTA GCTAAGGAGTTAGATCCCAGGTATGAAGACTATGTGGACCAGCATTATAAAGAGTTCCTCAAGAATCAG GGCAAAGTGGACTCGCTGGTGGGTGTGGATGTGATAGCTGCTTTGGACCTGTATGTGGAGCAGGGCCAG TGGGACAAGTGCATTGAAACAGCTACCAAGCAGAACTACAAGATTCTGCACAAGTATGTGGCTTTGTAT GCAACTCACTTGATCCGGGAGGGTAGCTCTGCCCAGGCATTGGCCCTGTATGTACAGCACGGAGCCCCT GCTAACCCACAGAACTTCAATATCTACAAAAGGATCTTCACTGACATGGTGAGCTCTCCTGGAACCAAC TGTGCCGAGGCCTATCATAGCTGGGCTGATCTTCGAGATGTCCTCTTCAACCTGTGTGAAAACCTGGTG AAGTCCAGTGAGGCAAACTCTCCAGCCCATGAGGAGTTCAAGACGATGCTGCTGATCGCTCATTACTAT GCCACGCGCTCTGCAGCCCAGAGTGTCAAACAGCTGGAAACCGTGGCTGCCAGGCTTTCTGTTTCACTC TTGCGTCACACCCAGCTACTACCTGTAGACAAAGCCTTCTATGAAGCAGGCATTGCTGCCAAGGCAGTT GGCTGGGATAACATGGCATTCATCTTCCTCAATCGCTTTTTGGACCTGACCGATGCAATCGAGGAAGGG ACTCTAGATGGCCTTGACCACTCTGATTTTCAGGATACAGACATTCCCTTTGAGGTGCCACTCCCAGCT AAGCAGCATGTACCGGAGGCTGAGAGAGAAGAGGTTCGAGACTGGGTGCTTACAGTCTCCATGGACCAG CGGCTGGAGCAGGTTCTGCCTCGGGATGAGCGTGGCGCCTACGAGGCCTCCCTAGTGGCAGCGAGCACT GGTGTTCGAGCCCTGCCCTGCCTTATTACAGGATACCCCATTCTGAGGAACAAAATTGAATTTAAGCGG CCAGGGAAGGCTGCTAACAAGGACAACTGGAATAAATTCCTTATGGCCATCAAGACCTCCCACAGCCCA GTGTGCCAGGACGTGCTGAAATTCATCAGTCAGTGGTGTGGAGGGCTCCCCAGCACCAGCTTTTCCTTT CAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015662
Insert Size	5250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015662.2
RefSeq Size	5488 bp
RefSeq ORF	5250 bp
Locus ID	26160
UniProt ID	Q9UG01
MW	197.6 kDa
Gene Summary	This gene encodes a subunit of the intraflagellar transport subcomplex IFT-B. Subcomplexes IFT-A and IFT-B are necessary for ciliary assembly and maintenance. Mutations in this gene have been associated with skeletal ciliopathies, with or without polydactyly, such as such short-rib thoracic dysplasias 1, 9 or 10. [provided by RefSeq, Mar 2014]

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