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Amphiphysin (AMPH) (NM_139316) Human Untagged Clone
CAT＃: SC310194
AMPH (untagged)-Human amphiphysin (AMPH), transcript variant 2
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMPH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310194 representing NM_139316.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGACATCAAGACGGGCATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAG GTCCTCCAAAAGCTGGGGAAAGCTGATGAGACAAAAGACGAACAGTTCGAAGAATATGTCCAGAACTTC AAACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAATCAAAGGC ATGCAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCCTGACTGGTATGGGCGG GAAGATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGAAGACTTCCATCAAAAACTCGTGGAT GGGTCCTTGCTAACACTGGATACCTACCTGGGGCAATTTCCTGACATAAAGAATCGCATCGCCAAGCGC AGCAGGAAGCTAGTGGACTATGACAGTGCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAG GATGAGAGTCGAATCTCTAAGGCAGAAGAAGAATTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTTAAC GTTGACTTACAAGAAGAGTTACCATCATTATGGTCAAGACGAGTTGGATTTTATGTTAATACTTTCAAA AACGTCTCCAGCCTTGAAGCCAAGTTTCATAAGGAAATTGCGGTGCTTTGCCACAAACTGTATGAAGTG ATGACAAAACTGGGTGACCAGCACGCCGACAAGGCCTTCACCATCCAAGGAGCGCCCAGTGATTCGGGT CCTCTCCGCATTGCAAAGACACCATCACCGCCTGAGGAGCCTTCACCCCTCCCGAGCCCGACAGCAAGT CCAAATCATACATTAGCACCTGCGTCTCCCGCACCAGCACGGCCTCGGTCACCTTCACAGACAAGGAAA GGGCCTCCTGTCCCACCTCTACCTAAAGTCACCCCGACAAAGGAACTGCAGCAGGAGAACATCATCAGT TTCTTTGAGGACAACTTTGTTCCAGAAATCAGTGTGACAACACCTTCCCAGAATGAAGTCCCTGAGGTG AAGAAAGAGGAGACTTTGCTGGATCTGGACTTTGATCCTTTCAAGCCCGAGGTGACACCTGCAGGTTCT GCTGGAGTGACCCACTCACCCATGTCTCAGACATTGCCCTGGGACCTATGGACGACAAGCACTGATTTG GTACAGCCGGCTTCTGGTGGTTCATTTAATGGATTCACACAGCCCCAGGATACTTCATTATTCACAATG CAGACAGACCAGAGTATGATCTGCAACTTGATCATACCTGGAGCTGATGCTGATGCAGCTGTTGGAACC TTGGTGTCAGCAGCTGAGGGGGCCCCAGGAGAGGAAGCAGAGGCGGAGAAGGCCACTGTCCCTGCCGGG GAAGGAGTAAGTTTAGAGGAGGCCAAAATTGGAACTGAAACCACTGAGGGTGCAGAGAGTGCCCAACCT GAAGCAGAGGAGCTCGAAGCAACAGTGCCTCAGGAGAAGGTCATTCCTTCGGTGGTCATAGAGCCTGCC TCCAACCATGAAGAGGAAGGAGAAAACGAAATAACTATAGGTGCAGAGCCCAAGGAGACCACCGAGGAC GCGGCTCCTCCGGGCCCCACCAGCGAGACACCGGAGCTGGCTACGGAGCAGAAGCCTATCCAGGACCCT CAGCCCACGCCTTCTGCACCAGCCATGGGGGCTGCTGACCAGCTAGCATCTGCAAGGGAGGCCTCTCAG GAATTGCCTCCTGGCTTTCTCTACAAGGTGGAAACACTGCATGATTTTGAGGCAGCAAATTCTGATGAA CTTACCTTACAAAGGGGTGATGTGGTGCTGGTGGTCCCCTCAGATTCAGAAGCTGATCAGGATGCAGGC TGGCTGGTGGGAGTGAAGGAATCAGACTGGCTTCAGTACAGAGACCTTGCCACCTACAAAGGCCTCTTT CCAGAGAACTTCACCCGACGCTTAGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_139316 |
| Insert Size | 1962 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139316.2 |
| RefSeq Size | 3300 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 273 |
| UniProt ID | P49418 |
| Domains | SH3, BAR |
| Protein Families | Secreted Protein |
| Protein Pathways | Fc gamma R-mediated phagocytosis |
| MW | 71.9 kDa |
| Gene Summary | This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010] Transcript Variant: This variant (2) lacks an in-frame exon within the coding region compared to variant 1, resulting in a shorter protein (isoform 2). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220048 | AMPH (Myc-DDK-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 4,560.00 |
|
| RC220048L3 | Lenti-ORF clone of AMPH (Myc-DDK-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 6,460.00 |
|
| RC220048L4 | Lenti-ORF clone of AMPH (mGFP-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 6,460.00 |
|
| RG220048 | AMPH (tGFP-tagged) - Human amphiphysin (AMPH), transcript variant 2 |
CNY 5,040.00 |
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