HAT1 (NM_003642) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KAT1
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_003642, the custom clone sequence may differ by one or more nucleotides ATGGCGGGATTTGGTGCTATGGAGAAATTTTTGGTAGAATATAAGAGTGCAGTGGAGAAG AAACTGGCAGAGTACAAATGTAACACCAACACAGCAATTGAACTAAAATTAGTTCGTTTT CCTGAAGATCTTGAAAATGACATTAGAACTTTCTTTCCTGAGTATACCCATCAACTCTTT GGGGATGATGAAACTGCTTTTGGTTACAAGGGTCTAAAGATCCTGTTATACTATATTGCT GGTAGCCTGTCAACAATGTTCCGTGTTGAATATGCATCTAAAGTTGATGAGAACTTTGAC TGTGTAGAGGCAGATGATGTTGAGGGCAAAATTAGACAAATCATTCCACCTGGATTTTGC ACAAACACGAATGATTTCCTTTCTTTACTGGAAAAGGAAGTTGATTTCAAGCCATTCGGA ACCTTACTTCATACCTACTCAGTTCTCAGTCCAACAGGAGGAGAAAACTTTACCTTTCAG ATATATAAGGCTGACATGACATGTAGAGGCTTTCGAGAATATCATGAAAGGCTTCAGACC TTTTTGATGTGGTTTATTGAAACTGCTAGCTTTATTGACGTGGATGATGAAAGATGGCAC TACTTTCTAGTATTTGAGAAGTATAATAAGGATGGAGCTACGCTCTTTGCGACCGTAGGC TACATGACAGTCTATAATTACTATGTGTACCCAGACAAAACCCGGCCACGTGTAAGTCAG ATGCTGATTTTGACTCCATTTCAAGGTCAAGGCCATGGTGCTCAACTTCTTGAAACAGTT CATAGATACTACACTGAATTTCCTACAGTTCTTGATATTACAGCGGAAGATCCATCCAAA AGCTATGTGAAATTACGAGACTTTGTGCTTGTGAAGCTTTGTCAAGATTTGCCCTGTTTT TCCCGGGAAAAATTAATGCAAGGATTCAATGAAGATATGGCGATAGAGGCACAACAGAAG TTCAAAATAAATAAGCAACACGCTAGAAGGGTTTATGAAATTCTTCGACTACTGGTAACT GACATGAGTGATGCCGAACAATACAGAAGCTACAGACTGGATATTAAAAGAAGACTAATT AGCCCATATAAGAAAAAGCAGAGAGATCTTGCTAAGATGAGAAAATGTCTCAGACCAGAA GAACTGACAAACCAGATGAACCAAATAGAAATAAGCATGCAACATGAACAGCTGGAAGAG AGTTTTCAGGAACTAGTGGAAGATTACCGGCGTGTTATTGAACGACTTGCTCAAGAGTAA
Restriction Sites	Please inquire
ACCN	NM_003642
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003642.2, NP_003633.1
RefSeq Size	1672 bp
RefSeq ORF	1260 bp
Locus ID	8520
UniProt ID	O14929
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.

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