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PCB (PC) (NM_001040716) Human Untagged Clone
CAT＃: SC311133
PC (untagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3
CNY 9,500.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PCB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC311133 representing NM_001040716.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCC GCTGCCTCCCCAAATGTCCGGCGCCTGGAGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGT GAGATTGCCATCCGTGTGTTCCGGGCCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAG CAGGACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCC GTGCAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCAC CCTGGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTT ATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTGGAGGCCCGGGCCATCGCCATTGCT GCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCCCTGCATGAGGCCCACGAGTTCTCC AACACCTACGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTG CACAGCTACGAGGAGCTGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAAT GGGGCGCTGTTTGTGGAGAAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACCAG TATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGAAGGTGGTCGAG ATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTGAAACTCGCT AAACAGGTGGGCTACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTC ATCGAGGTCAACTCCCGCCTGCAGGTGGAGCACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTC CATGCTCAGATCCACGTGGCTGAGGGCAGGAGCCTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGC ATCAACGGGTGTGCCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACC GGCCGCATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAA GGAGCCGTCATCTCGCCCCACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCC ACGGCCGCCACCAAGATGAGCAGGGCCCTTGCGGAGTTCCGCGTCCGAGGTGTGAAGACCAACATCGCC TTCCTGCAGAATGTGCTCAACAACCAGCAGTTCCTGGCAGGCACTGTGGACACCCAGTTCATCGACGAG AACCCAGAGCTGTTCCAGCTGCGGCCTGCACAGAACCGGGCCCAAAAGCTGTTGCACTACCTCGGCCAT GTCATGGTAAACGGTCCAACCACCCCGATTCCCGTCAAGGCCAGCCCCAGCCCCACGGACCCCGTTGTC CCTGCAGTGCCCATAGGCCCGCCCCCGGCTGGTTTCAGAGACATCCTGCTGCGAGAGGGGCCTGAGGGC TTTGCTCGAGCTGTGCGGAACCACCCGGGGCTGCTGCTGATGGACACGACCTTCAGGGACGCCCACCAG TCACTGCTGGCCACTCGTGTGCGCACCCACGATCTCAAAAAGATCGCCCCCTATGTTGCCCACAACTTC AGCAAGCTCTTCAGCATGGAGAACTGGGGAGGAGCCACGTTTGACGTCGCCATGCGCTTCCTGTATGAG TGCCCCTGGCGGCGGCTGCAGGAGCTCCGGGAGCTCATCCCCAACATCCCTTTCCAGATGCTGCTGCGG GGGGCCAATGCTGTGGGCTACACCAACTACCCAGACAACGTGGTCTTCAAGTTCTGTGAAGTGGCCAAA GAGAATGGCATGGATGTCTTCCGTGTGTTTGACTCCCTCAACTACTTGCCCAACATGCTGCTGGGCATG GAGGCGGCAGGAAGTGCCGGAGGCGTGGTGGAGGCTGCCATCTCATACACGGGCGACGTGGCCGACCCC AGCCGCACCAAGTACTCACTGCAGTACTACATGGGCTTGGCCGAAGAGCTGGTGCGAGCTGGCACCCAC ATCCTGTGCATCAAGGACATGGCCGGGCTGCTGAAGCCCACGGCCTGCACCATGCTGGTCAGCTCCCTC CGGGACCGCTTCCCCGACCTCCCACTGCACATCCACACCCACGACACGTCAGGGGCAGGCGTGGCAGCC ATGCTGGCCTGTGCCCAGGCTGGAGCTGATGTGGTGGATGTGGCAGCTGATTCCATGTCTGGGATGACT TCACAGCCCAGCATGGGGGCCCTGGTGGCCTGTACCAGAGGGACTCCCCTGGACACAGAGGTGCCCATG GAGCGCGTGTTTGACTACAGTGAGTACTGGGAGGGGGCTCGGGGACTGTACGCGGCCTTCGACTGCACG GCCACCATGAAGTCTGGCAACTCGGACGTGTATGAAAATGAGATCCCAGGGGGCCAGTACACCAACCTG CACTTCCAGGCCCACAGCATGGGGCTTGGCTCCAAGTTCAAGGAGGTCAAGAAGGCCTATGTGGAGGCC AACCAGATGCTGGGCGATCTCATCAAGGTGACGCCCTCCTCCAAGATCGTGGGGGACCTGGCCCAGTTT ATGGTGCAGAATGGATTGAGCCGGGCAGAGGCCGAAGCTCAGGCGGAAGAGCTGTCCTTTCCCCGCTCC GTGGTGGAGTTCCTGCAGGGCTACATCGGTGTCCCCCATGGGGGGTTCCCCGAACCCTTTCGCTCTAAG GTACTGAAGGACCTGCCAAGGGTGGAGGGGCGGCCTGGAGCCTCCCTCCCTCCCCTGGATCTGCAGGCA CTGGAGAAGGAGCTGGTAGACCGGCATGGGGAGGAGGTGACGCCGGAAGATGTGCTCTCAGCAGCTATG TACCCCGATGTGTTTGCCCACTTCAAGGACTTCACTGCCACCTTTGGCCCCCTGGATAGCCTGAATACT CGCCTCTTCCTGCAGGGACCCAAGATCGCAGAGGAGTTTGAGGTGGAGCTGGAGCGGGGCAAGACGCTG CACATCAAAGCCCTGGCCGTGAGCGACCTGAACCGGGCCGGCCAGAGGCAGGTCTTCTTTGAGCTCAAT GGGCAGCTGCGGTCCATCTTGGTCAAGGACACCCAGGCCATGAAGGAGATGCACTTCCACCCCAAGGCC CTAAAGGACGTGAAGGGCCAGATCGGGGCGCCCATGCCTGGGAAGGTGATAGACATCAAAGTGGTGGCA GGGGCCAAGGTGGCCAAGGGCCAGCCCCTGTGTGTGCTCAGTGCCATGAAGATGGAGACTGTGGTGACC TCACCCATGGAGGGTACTGTCCGCAAGGTTCATGTGACCAAGGACATGACACTGGAAGGTGACGACCTC ATCCTGGAGATCGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040716 |
| Insert Size | 3537 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040716.1 |
| RefSeq Size | 4192 bp |
| RefSeq ORF | 3537 bp |
| Locus ID | 5091 |
| UniProt ID | P11498 |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism |
| MW | 129.6 kDa |
| Gene Summary | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) also results from the use of a more distal promoter, however, it contains an additional 5' non-coding exon, hence has a longer 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221952 | PC (Myc-DDK-tagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 8,200.00 |
|
| RC221952L1 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC221952L2 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 11,500.00 |
|
| RC221952L3 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC221952L4 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 11,500.00 |
|
| RG221952 | PC (tGFP-tagged) - Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 10,550.00 |
