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MRPL42 (NM_172178) Human Untagged Clone
CAT＃: SC312098
MRPL42 (untagged)-Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 3
CNY 3,990.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPC204; MRP-L31; MRPL31; MRPS32; PTD007; RPML31 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC312098 representing NM_172178.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTGATGTCAAAGAGAACTATCTTGAAACATTTATTTCCAGTCCAAAATTCGGGCGTACATGTGCA GATGGAGCTTTATATTGTGTTTGTCATAAATCTACGTATTCTCCTCTACCAGATGACTATAATTGCAAC GTAGAGCTTGCTCTGACTTCTGATGGCAGGACAATAGTATGCTACCACCCTTCTGTGGACATTCCATAT GAACACACAAAACCTATCCCTCGGCCAGATCCTGTGCATAATAATGAAGAAACACATGATCAAGTGCTG AAAACCAGATTGGAAGAAAAAGTTGAACACCTTGAGGAAGGACCTATGATAGAACAACTTAGCAAAATG TTCTTTACTACTAAGCACCGTTGGTATCCTCATGGACGGTATCACAGATGTCGTAAGAATCTGAATCCT CCAAAAGACAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172178 |
| Insert Size | 429 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172178.2 |
| RefSeq Size | 2042 bp |
| RefSeq ORF | 429 bp |
| Locus ID | 28977 |
| MW | 16.6 kDa |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011] Transcript Variant: This variant (3) differs in the 5' UTR and internal coding region compared to variant 1. It encodes isoform b, which is the same length as isoform a, but it has a different N-terminus. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219347 | MRPL42 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 1,200.00 |
|
| RC219347L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219347L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG219347 | MRPL42 (tGFP-tagged) - Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 4,370.00 |
