Cullin 1 (CUL1) (AF062536) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF062536, the custom clone sequence may differ by one or more nucleotides ATGTCGTCAACCCGGAGCCAGAACCCCCACGGCCTGAAGCAGATTGGCCTGGACCAGATC TGGGACGACCTCAGAGCCGGCATCCAGCAGGTGTACACACGGCAGAGCATGGCCAAGTCC AGATATATGGAGCTCTACACTCATGTTTATAACTACTGTACTAGTGTTCACCAGTCAAAC CAAGCACGAGGAGCTGGAGTTCCTCCTTCTAAGTCGAAAAAGGGGCAGACACCTGGAGGA GCTCAGTTTGTTGGCCTGGAATTATATAAACGACTTAAGGAATTTTTGAAGAATTACTTG ACAAATCTTCTTAAGGATGGAGAAGATTTGATGGATGAGAGTGTACTGAAATTCTACACT CAACAATGGGAAGATTATCGATTTTCAAGCAAAGTGCTGAATGGAATTTGTGCCTACCTC AATAGACATTGGGTTCGCCGTGAATGTGACGAAGGACGAAAAGGAATATATGAAATCTAT TCGCTTGCATTGGTGACTTGGAGAGACTGTCTGTTCAGGCCACTGAATAAACAGGTAACA AATGCTGTTTTAAAGCTGATTGAAAAGGAAAGGAATGGTGAAACCATCAATACAAGATTG ATTAGTGGAGTTGTACAGTCTTACGTGGAATTGGGGCTGAATGAAGATGATGCATTTGCA AAGGGCCCTACGTTAACAGTGTATAAAGAATCCTTTGAATCTCAATTTTTGGCTGACACA GAGAGATTTTATACCAGAGAGAGTACTGAATTCTTGCAGCAGAACCCAGTTACTGAATAT ATGAAAAAGGCAGAGGCTCGTCTGCTTGAGGAACAACGAAGAGTTCAGGTTTACCTTCAT GAAAGCACACAAGATGAATTAGCAAGGAAATGTGAACAAGTCCTCATTGAAAAACACTTG GAAATTTTCCACACAGAATTTCAGAATTTATTGGATGCTGACAAAAATGAAGATTTGGGA CGCATGTATAATCTTGTATCTAGAATCCAGGATGGCCTAGGAGAATTGAAAAAACTGTTG GAGACACACATTCATAATCAGGGTCTTGCAGCCATTGAAAAGTGTGGAGAAGCTGCTTTA AATGACCCCAAAATGTATGTACAGACAGTGCTTGATGTTCATAAAAAATACAATGCCCTG GTAATGTCTGCATTCAACAATGACGCTGGCTTTGTGGCTGCTCTTGATAAGGCTTGTGGT CGCTTCATAAACAACAACGCGGTTACCAAGATGGCCCAATCATCCAGTAAATCCCCTGAG TTGCTGGCTCGATACTGTGACTCCTTGTTGAAGAAAAGTTCCAAGAACCCAGAGGAGGCA GAACTAGAAGACACACTCAATCAAGTGATGGTTGTCTTCAAGTACATAGAAGACAAAGAC GTATTTCAGAAGTTCTATGCGAAGATGCTCGCCAAGAGGCTCGTCCACCAGAACAGTGCA AGTGACGATGCCGAAGCCAGCATGATCTCCAAGTTAAAGCAAGCTTGCGGGTTCGAGTAC ACCTCTAAACTTCAGCGCATGTTTCAAGACATTGGCGTGAGCAAAGATCTGAACGAGCAA TTCAAAAAGCACTTGACAAACTCAGAACCCCTAGACTTGGATTTCAGCATTCAAGTGCTG AGCTCCGGGTCCTGGCCCTTCCAGCAGTCTTGTACATTTGCCTTGCCGTCAGAGTTGGAA CGTAGTTATCAGCGATTCACAGCTTTCTACGCCAGCCGCCACAGTGGCCGAAAATTGACG TGGTTATATCAGTTGTCTAAAGGAGAATTGGTAACTAACTGCTTCAAAAACAGATATACT TTGCAGGCGTCGACATTCCAGATGGCTATCCTGCTTCAGTACAACACGGAAGATGCCTAC ACTGTGCAGCAGCTGACCGACAGCACTCAAATTAAAATGGACATTTTGGCGCAAGTTTTA CAGATTTTATTAAAGTCGAAGCTATTGGTCTTGGAAGATGAAAATGCAAATGTTGATGAG GTGGAATTGAAGCCAGATACCTTAATAAAATTATATCTTGGTTATAAAAATAAGAAATTA AGGGTTAACATCAATGTGCCAATGAAAACCGAACAGAAGCAGGAACAAGAAACCACACAC AAAAACATCGAGGAAGACCGCAAACTACTGATTCAGGCGGCCATCGTGAGAATCATGAAG ATGAGGAAGGTTCTGAAACACCAGCAGTTACTTGGCGAGGTCCTCACTCAGCTGTCCTCC AGGTTCAAACCTCGAGTCCCTGTGATCAAGAAATGCATTGACATTCTAATTGAGAAAGAA TATTTGGAGCGAGTGGATGGTGAAAAGGACACCTACAGTTACTTGGCT
Restriction Sites	Please inquire
ACCN	AF062536
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF062536.1, AAC36681.1
RefSeq Size	2583 bp
RefSeq ORF	2583 bp
Locus ID	8454
Domains	CULLIN
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
Gene Summary	Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed:25704143, PubMed:25503564). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.