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ORP150 (HYOU1) (NM_006389) Human Untagged Clone
CAT＃: SC315056
HYOU1 (untagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 1
CNY 19,190.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315056 representing NM_006389.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGACAAAGTTAGGAGGCAGAGGCCGAGGAGGCGAGTCTGTTGGGCCTTGGTGGCTGTGCTCTTG GCAGACCTGTTGGCACTGAGTGATACACTGGCAGTGATGTCTGTGGACCTGGGCAGTGAGTCCATGAAG GTGGCCATTGTCAAACCTGGAGTGCCCATGGAAATTGTCTTGAATAAGGAATCTCGGAGGAAAACACCG GTGATCGTGACCCTGAAAGAAAATGAAAGATTCTTTGGAGACAGTGCAGCAAGCATGGCGATTAAGAAT CCAAAGGCTACGCTACGTTACTTCCAGCACCTCCTGGGGAAGCAGGCAGATAACCCCCATGTAGCTCTT TACCAGGCCCGCTTCCCGGAGCACGAGCTGACTTTCGACCCACAGAGGCAGACTGTGCACTTTCAGATC AGCTCGCAGCTGCAGTTCTCACCTGAGGAAGTGTTGGGCATGGTTCTCAATTATTCTCGTTCTCTAGCT GAAGATTTTGCAGAGCAGCCCATCAAGGATGCAGTGATCACCGTGCCAGTCTTCTTCAACCAGGCCGAG CGCCGAGCTGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAAGTGCTGCAGCTCATCAATGACAACACC GCCACTGCCCTCAGCTATGGTGTCTTCCGCCGGAAAGATATTAACACCACTGCCCAGAATATCATGTTC TATGACATGGGCTCAGGCAGCACCGTATGCACCATTGTGACCTACCAGATGGTGAAGACTAAGGAAGCT GGGATGCAGCCACAGCTGCAGATCCGGGGAGTAGGATTTGACCGTACCCTGGGGGGCCTGGAGATGGAG CTCCGGCTTCGAGAACGCCTGGCTGGGCTTTTCAATGAGCAGCGCAAGGGTCAGAGAGCAAAGGATGTG CGGGAGAACCCGCGTGCCATGGCCAAGCTGCTGCGTGAGGCTAATCGGCTCAAAACCGTCCTCAGTGCC AACGCTGACCACATGGCACAGATTGAAGGCCTGATGGATGATGTGGACTTCAAGGCAAAAGTGACTCGT GTGGAATTTGAGGAGTTGTGTGCAGACTTGTTTGAGCGGGTGCCTGGGCCTGTACAGCAGGCCCTCCAG AGTGCCGAAATGAGTCTGGATGAGATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGGGTCCCCAGA GTTCAGGAGGTGCTGCTGAAGGCCGTGGGCAAGGAGGAGCTGGGGAAGAACATCAATGCAGATGAAGCA GCCGCCATGGGGGCAGTGTACCAGGCAGCTGCGCTCAGCAAAGCCTTTAAAGTGAAGCCATTTGTCGTC CGAGATGCAGTGGTCTACCCCATCCTGGTGGAGTTCACGAGGGAGGTGGAGGAGGAGCCTGGGATTCAC AGCCTGAAGCACAATAAACGGGTACTCTTCTCTCGGATGGGGCCCTACCCTCAACGCAAAGTCATCACC TTTAACCGCTACAGCCATGATTTCAACTTCCACATCAACTACGGCGACCTGGGCTTCCTGGGGCCTGAA GATCTTCGGGTATTTGGCTCCCAGAATCTGACCACAGTGAAGCTAAAAGGGGTGGGTGACAGCTTCAAG AAGTATCCTGACTACGAGTCCAAGGGCATCAAGGCTCACTTCAACCTGGATGAGAGTGGCGTGCTCAGT CTAGACAGGGTGGAGTCTGTATTTGAGACACTGGTAGAGGACAGCGCAGAAGAGGAATCTACTCTCACC AAACTTGGCAACACCATTTCCAGCCTGTTTGGAGGCGGTACCACACCAGATGCCAAGGAGAATGGTACT GATACTGTCCAGGAGGAAGAGGAGAGCCCTGCAGAGGGGAGCAAGGACGAGCCTGGGGAGCAGGTGGAG CTCAAGGAGGAAGCTGAGGCCCCAGTGGAGGATGGCTCTCAGCCCCCACCCCCTGAACCTAAGGGAGAT GCAACCCCTGAGGGAGAAAAGGCCACAGAAAAAGAAAATGGGGACAAGTCTGAGGCCCAGAAACCAAGT GAGAAGGCAGAGGCAGGGCCTGAGGGCGTCGCTCCAGCCCCAGAGGGAGAGAAGAAGCAGAAGCCCGCC AGGAAGCGGCGAATGGTAGAGGAGATCGGGGTGGAGCTGGTTGTTCTGGACCTGCCTGACTTGCCAGAG GATAAGCTGGCTCAGTCGGTGCAGAAACTTCAGGACTTGACACTCCGAGACCTGGAGAAGCAGGAACGG GAAAAAGCTGCCAACAGCTTGGAAGCATTCATATTTGAGACCCAGGACAAGCTGTACCAGCCCGAGTAC CAGGAAGTGTCCACAGAGGAGCAGCGTGAGGAGATCTCTGGGAAGCTCAGCGCCGCATCCACCTGGCTG GAGGATGAGGGTGTTGGAGCCACCACAGTGATGTTGAAGGAGAAGCTGGCTGAGCTGAGGAAGCTGTGC CAAGGGCTGTTTTTTCGGGTAGAGGAGCGCAAGAAGTGGCCCGAACGGCTGTCTGCCCTCGATAATCTC CTCAACCATTCCAGCATGTTCCTCAAGGGGGCCCGGCTCATCCCAGAGATGGACCAGATCTTCACTGAG GTGGAGATGACAACGTTAGAGAAAGTCATCAATGAGACCTGGGCCTGGAAGAATGCAACTCTGGCCGAG CAGGCTAAGCTGCCCGCCACAGAGAAGCCTGTGTTGCTCTCAAAAGACATTGAAGCTAAGATGATGGCC CTGGACCGAGAGGTGCAGTATCTGCTCAATAAGGCCAAGTTTACCAAGCCCCGGCCCCGGCCTAAGGAC AAGAATGGGACCCGGGCAGAGCCACCCCTCAATGCCAGTGCCAGTGACCAGGGGGAGAAGGTCATCCCT CCAGCAGGCCAGACTGAAGATGCAGAGCCCATTTCAGAACCTGAGAAAGTAGAGACTGGATCCGAGCCA GGAGACACTGAGCCTTTGGAGTTAGGAGGTCCTGGAGCAGAACCTGAACAGAAAGAACAATCGACAGGA CAGAAGCGGCCTTTGAAGAACGACGAACTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_006389 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006389.4 |
| RefSeq Size | 4662 bp |
| RefSeq ORF | 3000 bp |
| Locus ID | 10525 |
| UniProt ID | Q9Y4L1 |
| Domains | HSP70 |
| Protein Families | Transmembrane |
| MW | 111.3 kDa |
| Gene Summary | The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) represents the longer transcript. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214178 | HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 1 |
CNY 10,976.00 |
|
| RC214178L3 | Lenti-ORF clone of HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 1 |
CNY 9,030.00 |
|
| RC214178L4 | Lenti-ORF clone of HYOU1 (mGFP-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 1 |
CNY 9,030.00 |
|
| RG214178 | HYOU1 (tGFP-tagged) - Human hypoxia up-regulated 1 (HYOU1), transcript variant 1 |
CNY 7,890.00 |
