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GRF2 (RAPGEF1) (NM_005312) Human Untagged Clone
CAT＃: SC315151
RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1
CNY 20,710.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C3G; GRF2 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_005312, the custom clone sequence may differ by one or more nucleotides
ATGGACACAGACTCTCAGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAA TTCCACTCACCCAAAATCAAGAGAACGCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCC GTAAAGATTCCAGAGAAGCCTGTGAACAAAGAGGCAACAGACAGATTTCTACCAGAGGGC TACCCTCTCCCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGTCCACCAGTGCTGTG GCTTCCAGGTCTCAAAGGCAGAAGAACCTGAGCTGGCTGGAGGAGAAAGAGAAGGAAGTT GTCAGTGCCCTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGATAAGAAGGTA CTGGAGATGCTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAG AACGATCCTCGAATTCAGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAA AGCCTCGCCAACCTCATTCGCTGGTCTGACCAAGTGATGCTGGAAGGCGTGAACTCAGAA GACAAGGAGATGGTGACGACTGTGAAGGGGGTCATCAAGGCTGTGCTGGATGGAGTGAAG GAGCTGGTCAGGCTCACCATCGAGAAGCAGGGACGTCCGTCTCCGACGAGCCCCGTGAAG CCCAGTTCCCCTGCCAGCAAGCCTGATGGCCCAGCAGAGCTCCCCCTGACAGACCGCGAG GTAGAGATCCTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCTCCCAGATGCC ACGGATGAAGAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAAT AGTCCTCCACCAGCATTGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTG GCTGTGGTGGCCCCCATGAGCCGAGCCACCAGTGGCTCCAGTTTGCCTGTTGGAATCAAT AGGCAGGATTTTGATGTTGACTGTTACGCACAGAGGCGACTGTCAGGAGGCAGCCACTCA TATGGTGGAGAGTCGCCCCGCCTCTCCCCCTGCAGCAGCATAGGCAAGCTCAGCAAGTCA GACGAGCAGCTGTCCTCTCTGGACAGGGACAGTGGGCAGTGCTCCCGGAACACAAGCTGT GAAACACTAGACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCTCTCTAACGCA GACCAGATACCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAG TCTGGGTCTCCATTTCTTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCA GACGGACCTCTGGCCCCAGGGCAGCAGACAGATACGCCACCTGCTCTCCCCGAGAAGAAG CGCAGGAGCGCAGCCTCCCAGACGGCGGACGGCTCTGGCTGCAGGGTGTCCTACGAGCGG CATCCCTCGCAGTATGACAACATCTCTGGGGAGGACCTGCAGAGCACAGCCCCGATCCCA TCCGTCCCCTACGCGCCCTTTGCTGCTATTCTGCCCTTTCAGCATGGAGGTTCCTCAGCC CCTGTCGAATTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCCAGAAAAACCA CCTCCTCTACCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGAC TACTCGGAGCCGCAGCCCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAG CAGAAGAACAAGCTCCTCATGGAGGTATACGGCTTCAGCGACTCCTTCAGTGGGGTGGAC TCCGTGCAGGAGCTGGCCCCGCCGCCCGCCCTACCCCCCAAGCAGCGGCAGCTGGAGCCA CCGGCTGGGAAAGACGGACATCCCAGAGATCCCTCAGCGGTCAGCGGCGTCCCTGGGAAG GACAGCAGAGACGGCAGTGAGAGGGCCCCAAAGTCACCAGATGCTCTGGAGTCGGCTCAG TCGGAGGAGGAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTATGTCCAGGCTG ACGCTCAAGCAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTA CTGGTCCATGCTACTGAGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTG ACCACCTACAGGACCTTCATCTCCCCAGAGGAGCTCATCAAGAAGCTGCAGTACAGATAT GAGAAATTCTCTCCCTTTGCCGACACATTCAAGAAGCGCGTCAGCAAGAACACGTTCTTC GTGCTGGTACGGGTGGTGGATGAGCTCTGCCTGGTGGAGTTGACAGAAGAGATCCTGAAG CTGCTGATGGAACTGGTCTTCCGCCTGGTGTGCAATGGGGAGCTGAGCCTGGCCCGTGTG CTCCGGAAGAACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTGTGCCACCTCC AGCCAGCCCCTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCAC AGCCATGAGATAGCGGAGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAG ATTCCTGAGGTTTTGCTTTGGGCAAAAGAGCAGAATGAGGAGAAGAGCCCCAACTTGACC CAGTTCACGGAGCACTTCAACAACATGTCCTACTGGGTCCGGTCCATAATCATGTTACAG GAAAAGGCCCAGGACAGGGAACGGCTGCTCTTGAAGTTCATCAAGATCATGAAGCACTTG CGGAAGCTGAATAACTTCAACTCCTACTTGGCCATCCTCTCTGCCCTGGACTCGGCGCCC ATCCGCAGGCTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTACTGCACACTG ATCGACAGCTCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCG TGCATCCCGTACCTGGGGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCA GACTACATCGACGGGAAAGTGAACTTCTCCAAGCGGTGGCAGCAGTTCAACATCCTCGAC AGCATGCGCTGCTTCCAGCAGGCGCACTATGACATGCGGAGGAACGACGACATTATAAAC TTCTTCAATGACTTCAGTGACCACCTGGCTGAGGAGGCCCTATGGGAACTGTCTCTGAAA ATTAAACCCAGGAACATAACAAGGAGAAAAACAGACCGGGAAGAGAAGACC |
| Restriction Sites | Please inquire |
| ACCN | NM_005312 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005312.2, NP_005303.2 |
| RefSeq Size | 6121 bp |
| RefSeq ORF | 3234 bp |
| Locus ID | 2889 |
| UniProt ID | Q13905 |
| Domains | RasGEFN, RasGEF |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma |
| Gene Summary | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) uses an alternate 5'-terminal exon which results in a distinct 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (a) has a shorter and distinct N-terminus, compared to isoform b. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221671 | RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 10,740.00 |
|
| RC221671L3 | Lenti-ORF clone of RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 12,640.00 |
|
| RC221671L4 | Lenti-ORF clone of RAPGEF1 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 12,640.00 |
|
| RG221671 | RAPGEF1 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 11,780.00 |
