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RASGRF1 (NM_002891) Human Untagged Clone
CAT＃: SC315326
RASGRF1 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1
CNY 24,510.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315326 representing NM_002891.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCCCTGGGACTGCTGGCGCGCAAGGACGGC ACGCGCAAAGGCTACCTGAGCAAGCGGAGTTCGGACAACACAAAATGGCAAACCAAGTGGTTCGCGCTG CTGCAGAACCTGCTCTTCTACTTCGAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAG GGCTGCGTCTGCGACCGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAG CATTACTTCACGGTGAACTTCAGCCATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGAGGACGCAAAA GATTGTGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACAGAGCATGAGGCA TTAATGCAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAGACCGTGGCCAAGCAGCTTCGG CAGCAGATCGAGGATGGGGAGATCGAGATCGAGCGGCTGAAGGCAGAGATCACATCCCTGCTCAAGGAC AATGAGCGCATCCAGTCCACCCAGACTGTCGCCCCCAACGATGAAGACAGCGACATCAAGAAAATTAAG AAGGTGCAGAGCTTCCTGCGGGGCTGGCTGTGCCGGCGGAAGTGGAAGACCATCATCCAGGACTACATC CGGTCACCCCATGCTGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGAGGCT GAGTACGTGCAGCAGCTGCACATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGATGGCCGCCAGCTCC AAGAAGCCTCCCATCACACACGACGACGTCAGCAGCATCTTCCTGAACAGCGAAACCATCATGTTTTTA CATCAGATCTTTTACCAAGGCCTGAAGGCCCGCATCTCCAGCTGGCCCACGCTGGTCCTGGCTGACCTA TTTGACATCCTGCTGCCCATGCTCAACATCTACCAAGAGTTCGTCCGCAACCACCAGTACAGCCTGCAG ATCCTGGCCCACTGCAAGCAGAACCGTGACTTCGACAAGCTGCTGAAGCACTACGAGGCCAAGCCTGAC TGCGAGGAGAGGACGCTGGAGACCTTCCTCACCTACCCCATGTTCCAGATCCCCAGGTACATCCTGACC CTCCATGAGCTCCTGGCCCACACGCCTCATGAGCACGTTGAGCGCAACAGCCTGGACTACGCCAAGTCC AAACTGGAGGAGCTGTCCAGAATAATGCACGATGAAGTAAGTGAGACGGAGAACATCCGGAAAAACCTG GCCATCGAGCGCATGATCATCGAAGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTTGTGAGACAA GGTTCCCTCATTCAGGTGCCCATGTCTGAAAAGGGCAAGATCACCAGGGGGCGCCTGGGGTCTCTCTCC CTAAAGAAAGAGGGCGAGCGACAGTGCTTCCTGTTTTCTAAGCATCTGATTATCTGTACCAGAGGCTCT GGAGGGAAGCTTCACTTGACCAAGAATGGAGTCATATCCCTCATTGACTGCACTTTATTGGAGGAGCCA GAAAGCACGGAGGAGGAAGCCAAAGGATCCGGCCAAGACATAGATCACTTGGATTTTAAAATCGGGGTG GAGCCAAAGGATTCCCCGCCCTTTACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAAGGCAGCGTGG ACCAGTGACATCAGCCAGTGTGTGGATAACATCCGATGCAATGGGCTCATGATGAACGCATTTGAAGAA AATTCCAAGGTCACTGTGCCGCAGATGATCAAGAGGACCAGGGAGGGGACCAGGGAAGCAGAAATGAGC AGGTCCGACGCCTCCTTATATTGTGATGATGTTGACATTCGCTTCAGCAAAACCATGAACTCCTGCAAA GTGCTGCAGATCCGCTACGCCAGTGTGGAGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTTCCTGAGC ATCGACTTCCTCAACACCTTCCTGCACTCCTACCGCGTCTTCACCACCGCCATCGTGGTCCTGGACAAG CTCATTACCATCTACAAGAAGCCTATCAGTGCCATTCCTGCCAGGTGGCTGAGGTCGCTGGAGCTCCTG TTTGCCAGTGGCCAGAACAATAAGCTCCTGTACGGTGAACCCCCCAAGTCCCCGCGCGCCACCCGCAAG TTCTCCTCGCCGCCACCTCTGTCCATCACCAAGACATCGTCACCGAGCCGCCGGCGGAAGCTCTCCCTG AACATCCCCATCATCACTGGCGGCAAGGCCCTGGACCTGGCCGCCCTCAGCTGCAACTCCAATGGCTAC ACCAGCATGTACTCGGCCATGTCACCCTTCAGCAAGGCCACGCTGGACACCAGCAAGCTCTATGTGTCC AGCAGCTTCACCAACAAGATTCCAGATGAGGGCGATACGACCCCTGAGAAGCCCGAAGACCCTTCAGCG CTCAGCAAGCAGAGCTCAGAAGTCTCCATGAGAGAGGAGTCAGATATTGATCAAAACCAGAGTGATGAT GGTGATACTGAAACATCACCAACTAAATCTCCAACAACACCCAAATCAGTCAAAAACAAAAATTCTTCA GAGTTCCCACTCTTTTCCTATAACAATGGAGTCGTCATGACCTCCTGTCGTGAACTGGACAATAACCGC AGTGCCTTGTCGGCCGCCTCTGCCTTTGCCATAGCAACCGCCGGGGCCAACGAGGGCACCCCAAACAAG GAGAAGTACCGGAGGATGTCCTTAGCCAGTGCAGGGTTTCCCCCAGACCAGAGGAATGGAGACAAGGAG TTTGTGATCCGCAGAGCAGCCACCAATCGTGTCTTGAACGTGCTCCGCCACTGGGTGTCCAAGCACTCT CAGGACTTTGAGACCAACGATGAGCTCAAATGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGCACGAC CCGGAGCTCCTGACCCAGGAGCGGAAGGCTGCAGCCAACATCATCAGGACTCTGACCCAGGAGGACCCA GGTGACAACCAGATCACGCTGGAGGAGATCACGCAGATGGCTGAAGGCGTGAAGGCTGAGCCCTTTGAA AACCACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCTGCTAGATCACCTCGTCTTCAAGAAGATTCCT TATGAGGAGTTCTTCGGACAAGGATGGATGAAACTGGAAAAGAATGAAAGGACCCCTTATATCATGAAA ACCACTAAGCACTTCAATGACATCAGTAACTTGATTGCTTCAGAAATCATCCGCAATGAGGACATCAAC GCCAGGGTGAGCGCCATCGAGAAGTGGGTGGCCGTAGCTGACATATGCCGCTGCCTCCACAACTACAAT GCCGTACTGGAGATCACCTCGTCCATGAACCGCAGTGCAATCTTCCGGCTCAAAAAGACGTGGCTCAAA GTCTCTAAGCAGACTAAAGCTTTGATTGATAAGCTCCAAAAGCTTGTGTCATCTGAGGGCAGATTTAAG AATCTCAGAGAAGCTCTGAAAAATTGTGACCCACCCTGTGTCCCTTACCTGGGGATGTACCTCACCGAC CTGGCCTTCATCGAGGAGGGGACGCCCAATTACACGGAAGACGGCCTGGTCAACTTCTCCAAGATGAGG ATGATATCCCATATTATCCGAGAGATTCGCCAGTTTCAACAAACTGCCTACAAAATAGAGCACCAAGCA AAGGTAACGCAATATTTACTGGACCAATCTTTTGTAATGGATGAAGAAAGCCTCTACGAGTCTTCTCTC CGAATAGAACCAAAACTCCCCACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002891 |
| Insert Size | 3822 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002891.4 |
| RefSeq Size | 6414 bp |
| RefSeq ORF | 3822 bp |
| Locus ID | 5923 |
| UniProt ID | Q13972 |
| Domains | IQ, RhoGEF, RasGEFN, PH, RasGEF |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, MAPK signaling pathway |
| MW | 145.2 kDa |
| Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219147 | RASGRF1 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1 |
CNY 8,864.00 |
|
| RC219147L3 | Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1, Myc-DDK-tagged |
CNY 14,630.00 |
|
| RC219147L4 | Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1, mGFP tagged |
CNY 14,630.00 |
|
| RG219147 | RASGRF1 (tGFP-tagged) - Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1 |
CNY 13,970.00 |
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