SART2 (DSE) (NM_001080976) Human Untagged Clone

CAT#: SC315973

DSE (untagged)-Human dermatan sulfate epimerase (DSE), transcript variant 2



  "NM_001080976" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
DSE Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DS-epi1; DSEP; DSEPI; EDSMC2; SART-2; SART2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC315973 representing NM_001080976.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGGACTCACACACGGGGGGCTCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTGTCAGCCTAC
ATCACCGACGAGAACCCAGAAGTTATGATTCCCTTCACCAATGCCAACTACGACAGCCATCCCATGCTG
TACTTCTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCTGCCAGCTCGCACGAGCACATTGCAGCC
CGCCTCACGGAGGCTGTGCACACGATGCTGTCCAGCCCCTTGGAATACCTCCCTCCCTGGGATCCCAAG
GACTACAGTGCCCGCTGGAATGAAATTTTTGGAAACAACTTGGGTGCCTTGGCAATGTTCTGTGTGCTG
TATCCTGAGAACATTGAAGCCCGAGACATGGCCAAAGACTACATGGAGAGGATGGCAGCGCAGCCTAGT
TGGTTGGTGAAAGATGCTCCTTGGGATGAGGTCCCGCTTGCTCACTCCCTGGTTGGTTTTGCCACTGCT
TATGACTTCTTGTACAACTACCTGAGCAAGACACAACAGGAGAAGTTTCTTGAAGTGATTGCCAATGCC
TCAGGGTATATGTATGAAACTTCATACAGGAGAGGATGGGGATTTCAATACCTGCACAATCATCAGCCC
ACCAACTGTATGGCTTTGCTCACGGGAAGCCTAGTCCTGATGAATCAAGGATATCTTCAAGAAGCCTAC
TTATGGACCAAACAAGTTCTGACCATCATGGAGAAATCTCTGGTCTTGCTCAGGGAGGTGACGGATGGC
TCCCTCTATGAAGGAGTTGCGTATGGCAGCTACACCACTAGATCACTCTTCCAATACATGTTTCTCGTC
CAGAGGCACTTCAACATCAACCACTTTGGCCATCCGTGGCTTAAACAACACTTTGCATTTATGTATAGA
ACCATCCTGCCAGGGTTTCAAAGGACTGTGGCTATTGCGGACTCAAATTACAACTGGTTTTATGGTCCA
GAAAGCCAATTAGTGTTCCTTGATAAATTTGTCATGCGTAATGGCAGTGGTAACTGGCTAGCTGACCAA
ATCAGAAGGAACCGTGTGGTGGAAGGTCCAGGAACACCATCCAAAGGGCAGCGCTGGTGCACTCTGCAC
ACAGAATTTCTCTGGTATGATGGCAGCTTGAAATCGGTTCCTCCTCCAGACTTTGGCACCCCTACACTG
CATTATTTTGAAGACTGGGGTGTCGTGACTTATGGAAGTGCACTACCTGCAGAAATCAATAGATCTTTC
CTTTCCTTCAAGTCTGGAAAACTGGGGGGACGTGCAATATATGACATTGTCCACAGAAACAAATACAAA
GATTGGATCAAAGGATGGAGAAATTTTAATGCAGGGCATGAACATCCTGATCAAAACTCATTTACTTTT
GCTCCCAATGGTGTGCCTTTCATTACTGAGGCTCTGTACGGGCCAAAGTACACCTTCTTCAACAATGTT
TTGATGTTTTCCCCAGCTGTGTCAAAGAGCTGCTTTTCTCCCTGGGTGGGTCAGGTCACAGAAGACTGC
TCATCAAAATGGTCTAAATACAAGCATGACCTGGCAGCTAGTTGTCAGGGGAGGGTGGTTGCAGCAGAG
GAGAAAAATGGGGTGGTTTTCATCCGAGGAGAAGGTGTGGGAGCTTATAACCCCCAGCTCAACCTGAAG
AATGTTCAGAGGAATCTCATCCTCCTACATCCACAGCTGCTTCTCCTTGTAGACCAAATACACCTGGGA
GAGGAGAGTCCCTTGGAGACAGCAGCGAGCTTCTTCCATAATGTGGATGTTCCTTTTGAGGAGACTGTG
GTAGATGGTGTCCATGGGGCTTTCATCAGGCAGAGAGATGGTCTCTATAAAATGTACTGGATGGACGAT
ACTGGCTACAGCGAGAAAGCAACCTTTGCCTCAGTGACATATCCTCGGGGCTATCCCTACAACGGGACA
AACTATGTGAATGTCACCATGCACCTCCGAAGTCCCATCACCAGGGCAGCTTACCTCTTCATAGGGCCA
TCTATAGATGTTCAGAGCTTCACTGTCCACGGAGACTCTCAGCAACTGGATGTGTTCATAGCCACCAGC
AAACATGCCTACGCCACATACCTGTGGACAGGTGAGGCCACAGGACAGTCTGCCTTTGCACAGGTCATT
GCTGATCGTCACAAAATTCTGTTTGACCGGAATTCAGCCATCAAGAGCAGCATTGTCCCTGAGGTGAAG
GACTATGCTGCTATTGTGGAACAGAACTTGCAGCATTTTAAACCAGTGTTTCAGCTGCTGGAGAAGCAG
ATACTGTCCCGAGTCCGGAACACAGCTAGCTTTAGGAAGACTGCTGAACGCCTGCTGAGATTTTCAGAT
AAGAGACAGACTGAGGAGGCCATTGACAGGATTTTTGCCATATCACAGCAACAGCAGCAGCAAAGCAAG
TCAAAGAAAAACCGAAGGGCAGGCAAACGCTATAAATTTGTGGATGCTGTCCCTGATATTTTTGCACAG
ATTGAAGTCAATGAGAAAAAGATTAGACAGAAAGCTCAGATTTTGGCACAGAAAGAACTACCCATAGAT
GAAGATGAAGAAATGAAAGACCTTTTAGATTTTGCAGATGTAACATACGAGAAACATAAAAATGGGGGC
TTGATTAAAGGCCGGTTTGGACAGGCACGGATGGTGACAACTACACACAGCAGGGCCCCATCACTGTCT
GCTTCCTATACCAGGTTGTTCCTGATTCTGAACATTGCTATTTTCTTTGTCATGTTGGCAATGCAACTG
ACTTATTTCCAGAGGGCCCAGAGCCTACATGGCCAAAGATGTCTTTATGCAGTTCTTCTCATAGATAGC
TGTATTTTATTATGGTTGTACTCTTCTTGTTCCCAATCACAGTGTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001080976
Insert Size 2877 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001080976.2
RefSeq Size 4092 bp
RefSeq ORF 2877 bp
Locus ID 29940
UniProt ID Q9UL01
Protein Families Transmembrane
Protein Pathways Chondroitin sulfate biosynthesis
MW 109.8 kDa
Gene Summary The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 all encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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