SEC16A (NM_014866) Human Untagged Clone

CAT#: SC315977

SEC16A (untagged)-Human SEC16 homolog A (S. cerevisiae) (SEC16A)



  "NM_014866" in other vectors (3)

CNY 45,410.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal anti-SEC16A antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms KIAA0310; p250; SEC16L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC315977 representing NM_014866.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGCCACCGCCCCAGACGGTCCCGTCTGGCATGGCTGGGCCACCTCCAGCCGGGAATCCTCGGAGC
GTGTTCTGGGCTAGCAGCCCTTACAGGAGACGGGCTAATAATAATGCAGCAGTGGCTCCGACAACTTGC
CCGTTGCAGCCGGTCACGGATCCATTTGCTTTTAGTAGACAGGCGCTCCAAAGTACACCACTGGGCAGT
TCGTCCAAAAGCAGTCCACCTGTCTTGCAAGGCCCAGCCCCCGCAGGGTTTTCTCAGCACCCCGGTTTG
CTTGTTCCTCACACACATGCCAGAGATAGCTCTCAGGGACCCTGTGAGCCCCTGCCTGGACCTCTGACA
CAGCCCAGAGCACATGCCAGTCCGTTTTCTGGTGCATTGACACCTTCAGCACCTCCTGGGCCTGAGATG
AACAGGAGTGCAGAGGTCGGTCCCAGTTCAGAGCCTGAAGTTCAGACTCTGCCATATCTTCCTCACTAC
ATTCCAGGAGTGGATCCTGAAACGTCTCATGGGGGCCACCCTCATGGGAACATGCCTGGGCTCGACCGA
CCCCTGAGCAGGCAAAACCCACATGACGGTGTGGTCACCCCAGCAGCATCCCCTTCCCTCCCTCAGCCT
GGTCTGCAGATGCCAGGACAGTGGGGGCCAGTGCAGGGAGGCCCACAGCCCTCGGGGCAACATCGTTCA
CCCTGCCCTGAAGGACCTGTTCCCAGCGGGGTGCCCTGTGCCACCAGCGTTCCTCATTTCCCCACCCCG
TCCATCCTACATCAGGGCCCTGGTCATGAGCAACACAGCCCTCTGGTGGCTCCCCCAGCAGCCTTGCCC
AGTGACGGAAGAGACGAGGTGAGCCACTTGCAAAGTGGAAGCCACCTGGCCAATAACTCTGATCCTGAA
AGTACATTCAGGCAAAATCCCAGAATTGTGAATCACTGGGCAAGCCCAGAGCTCAGGCAGAATCCAGGA
GTGAAGAATGAGCACCGGCCCGCCTCTGCTCTTGTGAACCCCCTCGCCCGGGGAGATAGCCCAGAAAAC
CGTACGCACCACCCACTGGGGGCTGGGGCCGGGTCTGGCTGTGCCCCGCTAGAAGCAGACTCAGGAGCT
TCAGGAGCTCTGGCGATGTTTTTCCAAGGGGGAGAGACAGAAAATGAGGAGAATCTCTCATCTGAAAAA
GCAGGCTTATCTGGTCAAGCGGACTTTGACGATTTCTGCTCCAGCCCTGGGCTAGGCCGTCCGCCCGCA
CCTACACACGTGGGGGCAGGCAGCCTCTGCCAGGCCCTTCTCCCAGGCCCCAGCAATGAGGCTGCTGGT
GATGTGTGGGGTGACACAGCGAGCACAGGGGTGCCGGATGCCAGCGGCTCGCAGTATGAGAATGTTGAG
AACTTAGAATTTGTTCAGAATCAAGAAGTTCTGCCAAGTGAGCCCCTCAATTTGGACCCTTCCTCCCCG
AGTGACCAGTTCAGATATGGGCCCCTTCCTGGGCCAGCTGTGCCCAGGCATGGTGCTGTGTGCCACACC
GGAGCCCCTGATGCCACACTGCATACAGTGCACCCTGACAGCGTGTCATCCAGCTATAGCAGCAGAAGC
CACGGAAGGCTCTCAGGCTCAGCCAGGCCCCAGGAGCTGGTTGGCACATTCATTCAGCAAGAAGTTGGA
AAACCCGAGGATGAAGCTTCAGGTAGTTTTTTTAAGCAAATCGATTCTTCTCCCGTAGGAGGTGAAACA
GACGAGACCACTGTGAGCCAGAATTACCGTGGCAGCGTGTCCCAGCCCTCAACCCCGAGCCCCCCGAAA
CCTACAGGAATATTTCAGACAAGTGCAAATAGTTCTTTCGAACCGGTAAAATCTCACTTAGTTGGGGTA
AAACCATTTGAGGCAGATCGCGCCAACGTGGTTGGTGAAGTAAGGGAGACCTGTGTCCGCCAGAAGCAG
TGCAGACCAGCTGCCGCCCTGCCCGATGCTTCCCCTGGCAACCTGGAGCAGCCACCAGACAACATGGAG
ACCCTCTGTGCACCCCAGGTCTGTCCCCTGCCTCTTAACTCCACCACGGAAGCTGTGCACATGCTTCCG
CACGCAGGGGCACCGCCCTTGGATACTGTGTATCCAGCACCCGAGAAGAGGCCTTCAGCCAGGACCCAG
GGGCCCGTGAAGTGTGAGAGCCCAGCAACGACTCTGTGGGCGCAAAGTGAGCTGCCAGATTTTGGAGGC
AACGTCCTTCTGGCCCCTGCAGCCCCGGCGCTTTATGTGTGTGCAAAACCTCAGCCACCTGTTGTTCAG
CCTCCAGAAGAGGCGATGTCCGGGCAGCAGTCACGGAACCCAAGCTCGGCGGCCCCGGTGCAGAGCCGA
GGTGGCATTGGTGCTTCTGAGAACCTTGAGAATCCTCCCAAAATGGGAGAGGAGGAGGCCCTTCAGTCC
CAGGCGAGTTCTGGTTATGCAAGTTTATTATCCTCACCGCCCACTGAGTCTCTGCAGAATCCTCCAGTC
TTGATTGCTCAGCCTGATCACAGCTATAATCTGGCTCAGCCCATTAACTTTTCTGTGTCCTTATCGAAC
TCTCATGAGAAGAATCAGTCCTGGAGAGAGGCTTTGGTGGGGGATAGACCTGCAGTCAGCAGTTGGGCT
CTCGGTGGTGATTCTGGGGAGAACACTTCTTTGTCTGGGATTCCAACCAGCTCTGTCCTTAGCTTGTCT
CTGCCTAGCAGTGTTGCCCAAAGTAATTTTCCACAAGGTTCTGGTGCTTCCGAAATGGTTTCTAATCAG
CCTGCTAATTTGCTGGTTCAACCACCATCCCAGCCAGTTCCAGAGAACTTGGTTCCAGAAAGTCAAAAG
GATCGTAAGGCAGGAAGTGCTCTTCCCGGATTTGCTAATAGCCCTGCTGGAAGCACAAGTGTGGTGTTA
GTTCCACCTGCACACGGCACCCTGGTGCCTGATGGTAATAAGGCAAACCATTCCAGTCATCAGGAAGAC
ACTTACGGAGCCCTAGACTTTACCTTAAGCAGGACTTTGGAAAATCCTGTAAACGTGTACAACCCGTCC
CATTCTGACAGCCTCGCTTCTCAGCAAAGTGTTGCCAGTCATCCCAGACAATCTGGGCCTGGGGCGCCT
AACCTTGACCGTTTTTATCAGCAGGTCACGAAAGATGCCCAGGGCCAGCCTGGCCTCGAAAGAGCCCAG
CAGGAGCTGGTGCCACCCCAGCAACAGGCTTCTCCCCCACAACTACCCAAAGCCATGTTTTCGGAGCTG
TCAAATCCAGAAAGTCTGCCCGCACAGGGACAGGCCCAGAACTCAGCACAGTCACCAGCAAGTCTGGTT
CTGGTCGACGCGGGTCAGCAGCTGCCCCCTCGGCCTCCTCAGTCCTCTAGCGTGTCTCTGGTGTCCAGT
GGCTCCGGCCAGGCAGCTGTGCCGTCAGAGCAGCCGTGGCCACAGCCAGTGCCTGCACTTGCCCCCGGC
CCACCGCCTCAGGACCTGGCCGCCTACTACTACTACCGGCCTTTGTACGATGCCTACCAGCCTCAGTAC
TCTTTGCCGTACCCACCGGAGCCTGGCGCAGCCTCCCTCTATTACCAGGATGTCTACAGCCTCTATGAG
CCTCGATACAGGCCCTATGATGGTGCTGCGTCTGCTTACGCCCAGAACTACCGCTATCCCGAGCCCGAG
CGGCCCAGCTCCCGAGCCAGCCACTCCTCGGAACGGCCACCTCCCAGGCAAGGATATCCTGAAGGATAC
TATAGTTCCAAAAGTGGATGGAGCAGTCAGAGCGATTACTATGCAAGCTATTACTCCAGCCAGTACGAT
TATGGAGATCCAGGTCACTGGGATCGTTACCACTACAGTGCTAGAGTCAGGGACCCCCGCACCTATGAC
CGGAGGTATTGGTGTGATGCAGAGTATGACGCATACAGGAGAGAGCACTCTGCCTTCGGGGACAGGCCC
GAGAAACGTGACAACAACTGGAGGTACGATCCTCGCTTCACGGGGAGTTTTGACGATGACCCCGATCCG
CACAGAGACCCTTATGGGGAAGAGGTGGACCGGCGCAGCGTCCACAGCGAGCACTCGGCACGGAGCCTG
CACAGCGCACACAGCCTGGCCAGCCGCCGCAGCAGCCTCAGCTCCCACTCGCACCAGAGTCAGATTTAC
AGAAGCCACAATGTGGCTGCCGGTTCCTACGAGGCCCCGCTTCCTCCAGGCTCCTTTCACGGCGATTTT
GCCTACGGCACCTACCGCAGCAATTTCAGCAGTGGCCCCGGCTTCCCAGAGTATGGCTACCCTGCCGAC
ACCGTCTGGCCTGCCATGGAGCAAGTTTCATCAAGACCAACTTCTCCTGAAAAATTTTCAGTGCCTCAT
GTCTGTGCCAGGTTTGGCCCTGGCGGTCAGCTTATCAAAGTGATTCCCAATCTGCCTTCAGAAGGACAG
CCGGCCTTGGTGGAGGTCCACAGCATGGAGGCCTTGCTGCAGCACACGTCTGAGCAGGAGGAGATGCGG
GCGTTCCCGGGACCCCTGGCCAAAGACGACACCCATAAGGTGGATGTCATTAATTTTGCACAGAACAAA
GCTATGAAATGTTTGCAGAATGAAAACTTAATTGACAAAGAGTCTGCAAGTCTTCTTTGGAATTTTATT
GTTCTCTTATGCAGACAAAATGGGACCGTGGTAGGGACCGACATTGCGGAGCTTCTGTTACGAGACCAC
AGAACAGTGTGGCTTCCTGGGAAGTCGCCCAATGAAGCAAACCTGATTGATTTCACGAATGAGGCAGTG
GAGCAGGTGGAAGAGGAGGAGTCTGGTGAGGCCCAGCTCTCTTTCCTCACTGGTGGTCCGGCGGCTGCC
GCCAGCTCGCTCGAGAGAGAGACCGAGAGGTTCAGGGAGCTGTTGCTGTATGGCCGTAAGAAGGATGCT
TTGGAGTCTGCAATGAAGAATGGCCTGTGGGGTCACGCTCTGCTACTTGCAAGTAAGATGGACAGCCGG
ACACACGCCCGAGTCATGACCAGGTTTGCTAACAGCCTCCCAATCAACGACCCTCTGCAGACAGTCTAC
CAGCTCATGTCCGGACGGATGCCTGCCGCGTCCACGTGCTGTGGAGACGAGAAATGGGGAGATTGGAGG
CCGCACCTCGCCATGGTCTTGTCCAACTTGAACAACAACATGGACGTCGAGTCCAGGACGATGGCTACC
ATGGGCGACACTCTGGCTTCAAGGGGCCTCTTGGATGCGGCCCACTTCTGCTACCTCATGGCCCAGGCG
GGATTTGGTGTTTACACGAAGAAAACTACAAAGCTTGTCTTAATCGGATCCAATCACAGTTTGCCATTC
TTAAAGTTCGCAACCAACGAAGCAATCCAGAGGACGGAAGCCTATGAGTACGCCCAGTCCCTGGGTGCC
GAGACCTGCCCCCTGCCTAGTTTCCAGGTGTTTAAGTTCATCTACTCCTGCCGCCTGGCGGAAATGGGG
CTGGCCACGCAAGCCTTCCACTACTGTGAGGCCATCGCGAAGAGCATCCTGACGCAGCCGCACCTGTAT
TCCCCGGTGTTGATCAGCCAGCTTGTGCAGATGGCTTCCCAGTTACGACTCTTCGATCCCCAGCTGAAA
GAGAAGCCAGAAGAGGAGTCCTTGGCCGCACCCACGTGGCTGGTTCACCTGCAGCAGGTGGAGCGGCAG
ATTAAGGAGGGGGCTGGAGTATGGCATCAGGATGGAGCCCTCCCGCAGCAGTGTCCTGGCACTCCGAGT
TCCGAGATGGAGCAGTTGGACAGGCCAGGACTCAGTCAGCCAGGAGCCCTGGGGATCGCCAACCCTCTG
CTGGCGGTGCCTGCACCGAGCCCTGAGCACTCGAGCCCGAGCGTGCGGCTGCTGCCCTCAGCTCCGCAG
ACGCTCCCTGACGGCCCATTGGCCAGTCCTGCCAGAGTGCCGATGTTCCCAGTGCCACTGCCCCCGGGG
CCCCTGGAGCCGGGTCCTGGCTGTGTGACCCCAGGGCCTGCACTTGGCTTCCTGGAGCCCTCCGGGCCT
GGCCTCCCACCTGGTGTGCCACCTCTGCAGGAAAGGAGACACTTGCTCCAGGAAGCCAGGAGCCCAGAC
CCAGGGATAGTGCCGCAGGAGGCGCCTGTTGGAAACTCACTTTCCGAGCTAAGCGAAGAAAATTTTGAT
GGAAAATTTGCTAATCTGACCCCCTCGAGGACGGTGCCAGACTCGGAGGCCCCCCCAGGGTGGGATCGT
GCCGACTCGGGTCCCACGCAGCCACCTCTGTCTCTCTCACCCGCTCCCGAAACAAAGAGACCCGGACAG
GCAGCCAAGAAAGAAACGAAGGAACCTAAGAAGGGTGAATCCTGGTTCTTTCGTTGGCTACCTGGAAAG
AAAAAGACAGAAGCTTATTTGCCAGATGACAAGAACAAATCGATTGTTTGGGATGAAAAGAAAAACCAG
TGGGTGAATTTAAATGAGCCAGAAGAGGAGAAGAAAGCCCCGCCCCCACCTCCAACCTCGATGCCCAAG
ACTGTGCAAGCTGCCCCGCCTGCCCTCCCAGGGCCTCCTGGAGCCCCCGTGAACATGTACTCTAGAAGA
GCAGCAGGAACCAGAGCTCGCTACGTTGACGTCCTGAACCCAAGCGGGACCCAGCGGAGCGAGCCGGCT
CTCGCTCCTGCGGACTTTGTCGCTCCACTCGCGCCACTCCCAATTCCTTCTAACTTGTTCGTGCCAACC
CCAGATGCAGAAGAACCACAGCTTCCAGACGGGACTGGCAGGGAAGGGCCTGCAGCAGCTAGGGGCCTG
GCCAATCCAGAGCCTGCCCCAGAGCCCAAGGTTTTAAGCTCTGCAGCGTCACTCCCTGGCTCTGAACTC
CCCTCCTCCAGGCCTGAGGGTTCCCAGGGAGGAGAGCTTTCGCGCTGTAGTTCAATGAGTTCATTATCA
CGTGAAGTGAGCCAGCATTTTAATCAGGCTCCTGGCGACCTCCCTGCTGCAGGGGGCCCTCCCAGCGGG
GCCATGCCCTTCTACAACCCTGCTCAGCTGGCACAGGCCTGCGCCACCTCCGGGAGCTCAAGGCTAGGG
AGGATTGGCCAGAGGAAGCACCTGGTGCTGAACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_014866
Insert Size 7074 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014866.1
RefSeq Size 9059 bp
RefSeq ORF 7074 bp
Locus ID 9919
UniProt ID O15027
MW 251.9 kDa
Gene Summary This gene encodes a protein that forms part of the Sec16 complex. This protein has a role in protein transport from the endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle formation at the transitional ER. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Feb 2013]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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