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MMACHC (NM_015506) Human Untagged Clone
CAT＃: SC316303
MMACHC (untagged)-Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC)
CNY 6,270.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cblC |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_015506 edited
ATGGAGCCGAAAGTCGCAGAGCTGAAGCAGAAGATCGAGGACACGCTATGTCCTTTTGGC TTCGAGGTTTACCCCTTCCAGGTGGCATGGTACAATGAACTCTTGCCTCCAGCCTTCCAC CTACCGCTGCCAGGACCTACCCTGGCCTTCCTGGTACTCAGCACGCCTGCCATGTTTGAC CGGGCCCTCAAGCCCTTCTTGCAGAGCTGCCACCTCCGAATGCTGACTGACCCAGTGGAC CAGTGTGTGGCCTACCATCTGGGCCGTGTTAGAGAGAGCCTCCCAGAGCTGCAGATAGAA ATCATTGCTGACTACGAGGTGCACCCCAACCGACGCCCCAAGATCCTGGCCCAGACAGCA GCCCATGTAGCTGGGGCTGCTTACTACTACCAACGACAAGATGTGGAGGCTGACCCATGG GGGAACCAGCGCATATCAGGTGTGTGCATACACCCCCGATTTGGGGGCTGGTTTGCCATC CGAGGGGTAGTGCTGCTGCCAGGGATAGAGGTGCCAGATCTGCCACCCAGAAAACCTCAT GACTGTGTACCTACAAGAGCTGACCGTATCGCCCTACTCGAAGGCTTCAATTTCCACTGG CGTGATTGGACTTACCGGGATGCTGTGACACCCCAGGAGCGCTACTCAGAAGAGCAGAAG GCCTACTTCTCCACTCCACCTGCCCAACGATTGGCCCTATTGGGCTTGGCTCAGCCCTCA GAGAAGCCTAGTTCTCCCTCCCCGGACCTTCCCTTTACCACACCCGCCCCCAAGAAGCCT GGGAATCCCAGCAGAGCCCGGAGCTGGCTCAGCCCCAGGGTCTCACCACCTGCATCCCCT GGCCCTTGATTTTCTCCCATGTGGACCCTGATTTATGGTGGTACTTGCTAGGACTTAATT GGCTTTGGCAAAGCAAAAGGTTTTGAGTACAAGATTACTATTTTTGATAATATAGTAGAG ATCTTCCATGAAGATAACAAGACTATCTCTAATCAAGGCTAGAACCAAGGGAAGGCTAAG AATTGCCCAGTACTGTGCAACTACGAAAGCCCTACCCAAGGCCACCAGCCTTGTCTTCCT CTTTCCTCTGTCAGTTCAAAAAGAACAGAAACCTCCAGCTCTTTTACATAGCAGGTACCA GGCATTTATCAGAAGAGGCCAAGCTTCTGGTTCCCATGCAGCCCTTTGAATAGTGTGTCT AAACAAAAATAGGTGTCCAAGTAGTCACACTGAGACTTTAACTGGTAACCCAGCCTGTGG CGTCAGTCGCAGTGCTCTGGCCAACACTATAGCAGGGCTTATTCTTCTCCCTCATGTGTA GTGAAACAAAATGTAACACCTTGGGTTCATTCAGTTCCATTCCCTATATCTACCTGTGTC AATATAATTCCCTGATTTGGAGGCAGCTCTCCTCATTTTCCCCAAAACAGGGAAAGCAAG GAGTAAATTCCTCTTAAAATCAAAAGCTAATAATATGCTTCCTAAAATAAAGACTCATCA AGGTCTCAGTTCAAGTTTAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_015506 unedited
GTGCACTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGATGGAGCCGAA AGTCGCAGAGCTGAAGCAGAAGATCGAGGACACGCTATGTCCTTTTGGCTTCGAGGTTTA CCCCTTCCAGGTGGCATGGTACAATGAACTCTTGCCTCCAGCCTTCCACCTACCGCTGCC AGGACCTACCCTGGCCTTCCTGGTACTCAGCACGCCTGCCATGTTTGACCGGGCCCTCAA GCCCTTCTTGCAGAGCTGCCACCTCCGAATGCTGACTGACCCAGTGGACCAGTGTGTGGC CTACCATCTGGGCCGTGTTAGAGAGAGCCTCCCAGAGCTGCAGATAGAAATCATTGCTGA CTACGAGGTGCACCCCAACCGACGCCCCAAGATCCTGGCCCAGACAGCAGCCCATGTAGC TGGGGCTGCTTACTACTACCAACGACAAGATGTGGAGGCTGACCCATGGGGGAACCAGCG CATATCAGGTGTGTGCATACACCCCCGATTTGGGGGCTGGTTTGCCATCCGAGGGGTAGT GCTGCTGCCAGGGATAGAGGTGCCAGATCTGCCACCCAGAAAACCTCATGACTGTGTACC TACAAGAGCTGACCGTATCGCCCTACTCGAAGGCTTCAATTTCCACTGGCGTGATTGGAC TTACCGGGATGCTGTGACACCCCAGGAGCGCTACTCAGAAGAGCAGAACGCCTACTTCTC CACTCCACCTGCCCAACGATTGGCCCTATTGGGCTTGGCTCAGCCCTCAGAGAAGCCTAG TTCTCCCTCCCCGACCTTCCCTTTACCACACCCGCCCCCAGAAGCCTGGGAATCCCAGCA GAGCCCGGAGCTGGCTCAGCCCCAGGGTCTCACCACCTGCATCCCCTGGCCCTTGATTTT TCTCCCATGTG >Forward primer walk for NM_015506 unedited
CCCCGTACAGGGGGCCGGTTTGCCTCCGGGGGTAGCGCTTGCTTGCCAGGGATAGAGGTG CCAGACTCTGCCACCCAGAAAACCTCATGACTGTGTACCTACAAGAGCTGACCGTATCGC CCTACTCGAAGGCTTCAATTTCCACTGGCGTGATTGGACTTACCGGGATGCTGTGACACC CCAGGAGCGCTACTCAGAAGAGCAGAAGGCCTACTTCTCCACTCCACCTGCCCAACGATT GGCCCTATTGGGCTTGGCTCAGCCCTCAGAGAAGCCTAGTTCTCCCTCCCCGGACCTTCC CTTTACCACACCCGCCCCCAAGAAGCCTGGGAATCCCAGCAGAGCCCGGAGCTGGCTCAG CCCCAGGGTCTCACCACCTGCATCCCCTGGCCCTTGATTTTCTCCCATGTGGACCCTGAT TTATGGTGGTACTTGCTAGGACTTAATTGGCTTTGGCAAAGCAAAAGGTTTTGAGTACAA GATTACTATTTTTGATAATATAGTAGAGATCTTCCATGAAGATAACAAGACTATCTCTAA TCAAGGCTAGAACCAAGGGAAGGCTAAGAATTGCCCAGTACTGTGCAACTACGAAAGCCC TACCCAAGGCCACCAGCCTTGTCTTCCTCTTTCCTCTGTCAGTTCAAAAAGAACAGAAAC CTCCAGCTCTTTTACATAGCAGTACCAGGCATTTATCAGAAGAGGCCAAGCTTCTGGTTC CCATGCAGCCCTTTGAATAGTGTGTCTAAACAAAAATAGGTGTCCAAGTAGTCACACTGA GACTTTAACTGGTAACCCAGCCTGTGGCGTCAGTCGCAGTGCTCTGGCCAACACTATAGC AGGCTTATTCTTCTCCCTCATGTGTAGTGAAACAAAAT |
| Restriction Sites | Please inquire |
| ACCN | NM_015506 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015506.1, NP_056321.1 |
| RefSeq Size | 2247 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 25974 |
| UniProt ID | Q9Y4U1 |
| Gene Summary | The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mechanism of vitamin B12-responsiveness in cblC methylmalonic aciduria with homocystinuria
,D.S. Froesea, J. Zhanga, S. Healya and R.A. Grave,
doi:10.1016/j.ymgme.2009.07.014
[MMACHC]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223846 | MMACHC (Myc-DDK-tagged)-Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC) |
CNY 2,400.00 |
|
| RC223846L1 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223846L2 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC), mGFP tagged |
CNY 5,890.00 |
|
| RC223846L3 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223846L4 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC), mGFP tagged |
CNY 4,800.00 |
|
| RG223846 | MMACHC (tGFP-tagged) - Human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC) |
CNY 4,000.00 |
