MYH15 (NM_014981) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC316613 representing NM_014981. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTTGAATCTTGCCTACTCACGTTTAGGGCCTTTTTTTGGTGGATTGCTTTAATAAAGATGGATCTG TCAGACCTTGGAGAAGCCGCAGCCTTCCTCAGAAGAAGTGAAGCTGAGCTGCTTCTACTACAGGCCACA GCCTTGGATGGGAAGAAGAAATGCTGGATTCCTGATGGTGAGAATGCTTATATCGAGGCTGAGGTAAAA GGGAGTGAAGATGATGGAACAGTAATTGTTGAGACAGCAGATGGAGAGAGTCTGAGCATAAAGGAGGAC AAAATCCAGCAGATGAATCCTCCAGAGTTTGAAATGATTGAAGACATGGCAATGCTGACTCACCTCAAT GAGGCATCCGTGCTGCATACCCTGAAGCGGCGCTATGGCCAGTGGATGATCTATACATATTCAGGTCTC TTCTGTGTGACCATAAACCCTTACAAATGGCTTCCCGTGTATCAGAAAGAAGTCATGGCCGCCTACAAA GGGAAGAGGCGATCAGAGGCTCCCCCTCACATCTTTGCTGTTGCCAATAACGCCTTTCAGGACATGCTT CACAATCGAGAAAATCAGTCTATACTCTTCACAGGAGAATCTGGTGCTGGAAAGACTGTGAACAGCAAA CATATTATCCAGTATTTTGCCACCATAGCAGCCATGATTGAATCCAGGAAAAAGCAGGGGGCGTTAGAA GATCAAATCATGCAAGCGAATACTATCTTGGAAGCATTTGGAAATGCTAAAACCCTGAGAAATGACAAC TCCTCTCGTTTTGGCAAATTCATCAGGATGCACTTTGGTGCCAGAGGCATGCTGTCATCTGTGGACATT GATATCTATTTGCTTGAAAAGTCCAGGGTGATTTTCCAGCAGGCTGGAGAGAGGAACTACCACATATTC TATCAAATTCTATCTGGACAAAAAGAGCTTCATGACCTGCTCCTGGTATCTGCAAATCCCTCAGACTTC CACTTTTGCTCCTGTGGAGCAGTTACTGTGGAGAGCTTGGATGATGCTGAAGAATTGCTGGCCACAGAA CAAGCCATGGACATCTTGGGCTTTCTTCCTGATGAGAAGTATGGATGCTATAAACTCACTGGAGCCATC ATGCACTTTGGAAATATGAAATTTAAACAGAAACCTAGAGAAGAGCAACTGGAAGCAGATGGCACAGAA AATGCTGACAAAGCTGCTTTCCTCATGGGCATTAACTCCTCTGAGTTGGTAAAGTGCTTGATCCATCCT AGAATCAAAGTTGGTAACGAATATGTTACCAGAGGTCAAACTATAGAACAGGTAACCTGTGCTGTCGGT GCCCTGTCCAAGTCAATGTATGAAAGGATGTTTAAGTGGCTAGTGGCACGGATCAACAGGGCCCTGGAT GCCAAGCTGTCAAGGCAGTTCTTCATTGGCATTCTTGACATCACTGGTTTTGAAATCCTTGAGTATAAT AGCCTTGAGCAACTTTGCATTAATTTTACCAATGAAAAATTACAACAATTCTTCAATTGGCACATGTTT GTTCTGGAGCAAGAGGAATATAAGAAAGAAAGCATTGAATGGGTGTCTATTGGCTTTGGTCTGGATTTG CAAGCTTGCATAGATCTCATTGAGAAGCCAATGGGCATCCTTTCCATCCTTGAAGAAGAGTGTATGTTT CCTAAGGCTACAGACCTGACTTTCAAGACCAAACTCTTTGACAACCATTTTGGAAAGTCGGTTCATCTC CAGAAGCCCAAGCCTGATAAGAAGAAATTTGAAGCTCATTTTGAACTTGTCCATTATGCAGGAGTGGTA CCTTATAATATCAGTGGTTGGCTGGAAAAGAACAAAGACCTCCTTAATGAAACAGTGGTAGCTGTATTT CAGAAGTCTTCCAACAGACTCCTGGCGAGCCTTTTTGAAAATTACATGAGTACTGACAGTGCTATACCA TTTGGGGAGAAGAAACGAAAGAAAGGAGCTTCATTCCAAACGGTTGCATCTCTGCATAAAGAAAACCTG AATAAATTGATGACTAATCTGAAATCAACAGCACCTCATTTTGTGAGATGCATAAATCCCAATGTGAAC AAAATACCAGGTATACTGGACCCTTACTTGGTTCTACAGCAGTTGCGCTGTAATGGTGTCTTGGAAGGG ACTAGGATATGCCGTGAAGGTTTTCCAAACCGACTGCAGTATGCTGATTTTAAACAAAGGTACTGCATT CTGAATCCAAGGACCTTTCCAAAGAGCAAGTTTGTGAGCAGCAGAAAAGCAGCTGAAGAATTACTTGGC TCCTTGGAGATAGACCATACCCAGTACCGATTTGGAATCACTAAGGTGTTTTTTAAAGCTGGGTTTCTG GGCCAACTGGAAGCAATAAGAGATGAGAGACTATCTAAAGTCTTCACATTGTTCCAAGCCAGAGCACAG GGCAAACTGATGCGAATCAAATTCCAGAAGATTCTGGAAGAAAGGGATGCACTTATTTTGATCCAATGG AACATAAGAGCTTTCATGGCTGTGAAGAACTGGCCCTGGATGAGGCTCTTCTTCAAGATCAAGCCTCTT GTTAAATCTTCAGAAGTAGGAGAAGAAGTAGCTGGACTGAAGGAAGAGTGTGCACAATTACAGAAAGCC TTGGAGAAATCAGAGTTTCAGAGGGAGGAACTGAAAGCAAAGCAAGTATCCCTCACTCAGGAAAAAAAT GACCTGATTCTTCAGCTTCAGGCTGAGCAAGAGACACTGGCAAATGTTGAAGAGCAGTGCGAGTGGCTG ATTAAATCCAAGATCCAGCTGGAGGCCAGAGTAAAGGAGCTGTCGGAGAGGGTGGAGGAAGAAGAGGAG ATAAATTCTGAGCTGACTGCCAGGGGGCGGAAACTCGAAGATGAATGTTTTGAGTTGAAGAAAGAAATC GATGACCTGGAAACAATGTTGGTGAAGTCAGAGAAGGAGAAGCGTACTACAGAGCACAAGGTCAAGAAC TTGACTGAGGAAGTAGAGTTTCTAAATGAGGATATCAGCAAACTTAACAGAGCAGCCAAGGTTGTGCAG GAGGCCCATCAGCAGACCCTGGATGACCTGCACATGGAGGAGGAGAAGCTCAGCAGCCTGAGCAAAGCA AATCTGAAGCTGGAACAGCAAGTTGATGAGCTTGAGGGTGCCCTTGAGCAGGAGAGAAAAGCGAGAATG AACTGTGAAAGGGAACTGCACAAACTGGAGGGCAATTTAAAGCTGAATCGGGAAAGTATGGAGAACCTG GAAAGCAGCCAGCGACACCTGGCAGAAGAGCTGAGGAAAAAAGAATTAGAATTGAGTCAGATGAATTCA AAAGTGGAGAATGAGAAAGGCCTGGTAGCTCAGCTTCAGAAGACGGTTAAAGAGCTTCAGACTCAAATA AAGGATTTGAAAGAGAAACTAGAAGCTGAAAGGACCACTCGAGCCAAGATGGAAAGGGAGAGAGCTGAC CTCACCCAAGACCTGGCTGACTTGAATGAGAGGCTGGAGGAGGTAGGAGGATCCAGTTTGGCTCAGCTG GAAATAACTAAGAAACAGGAAACCAAATTCCAGAAGCTGCACCGAGACATGGAAGAGGCCACTCTGCAC TTTGAGACAACTTCTGCATCTTTGAAGAAGAGACATGCAGACAGCCTGGCTGAGCTCGAGGGCCAGGTA GAAAATCTACAGCAGGTCAAGCAGAAACTGGAAAAAGACAAGAGTGACTTGCAGCTAGAAGTAGATGAC CTCCTGACCCGTGTTGAGCAGATGACAAGAGCTAAGGCAAATGCTGAGAAACTCTGTACTCTATATGAA GAGCGCTTGCATGAAGCAACTGCAAAGCTAGATAAGGTGACTCAGTTGGCAAATGACCTGGCAGCACAA AAGACAAAGCTGTGGAGTGAGAGTGGCGAGTTCCTACGGAGGCTTGAAGAGAAGGAGGCTCTGATAAAC CAACTTTCCAGGGAAAAGAGCAACTTCACTCGGCAGATTGAAGACCTGAGAGGGCAGCTGGAAAAGGAG ACCAAATCCCAGAGTGCCCTGGCCCATGCCCTGCAGAAGGCTCAGCGTGACTGTGACCTTCTACGAGAG CAGTATGAGGAAGAACAAGAGGTCAAGGCTGAGCTGCACCGGACCTTATCCAAAGTCAATGCTGAAATG GTGCAATGGAGAATGAAGTATGAAAACAATGTCATCCAGAGAACAGAAGACTTGGAGGATGCCAAGAAG GAACTGGCAATTAGATTGCAGGAGGCAGCCGAAGCCATGGGGGTGGCCAATGCCAGAAATGCCTCCTTG GAGAGAGCCAGGCACCAGCTGCAGCTGGAGCTCGGGGACGCCCTGTCTGACCTCGGGAAGGTCCGCTCT GCAGCAGCCAGGCTGGACCAGAAGCAGCTGCAGTCTGGCAAGGCCCTTGCCGACTGGAAGCAGAAGCAC GAGGAGTCCCAGGCGTTGCTGGATGCCTCTCAGAAGGAAGTTCAGGCTCTCAGTACAGAGCTCCTCAAG CTCAAGAACACCTATGAGGAGAGCATCGTGGGCCAGGAGACACTCAGGAGGGAGAACAAGAACCTCCAA GAAGAGATTTCTAATCTGACAAACCAGGTTAGAGAAGGGACCAAGAACTTAACTGAAATGGAAAAGGTC AAGAAACTAATTGAAGAAGAGAAGACAGAAGTCCAGGTGACACTGGAAGAAACAGAGGGAGCCCTGGAA CGTAATGAAAGCAAGATTCTTCATTTCCAGCTTGAACTCTTGGAAGCTAAAGCAGAACTTGAAAGAAAG CTTTCAGAGAAAGATGAAGAAATAGAAAATTTTAGGAGGAAGCAGCAGTGTACCATTGACTCCCTGCAG TCTAGTCTGGATTCTGAAGCTAAGAGCAGAATTGAGGTTACCCGGCTGAAGAAGAAGATGGAAGAGGAC CTCAATGAGATGGAACTCCAGCTTAGCTGTGCCAACCGGCAGGTGTCAGAAGCAACCAAATCCCTGGGC CAGCTTCAGATTCAAATCAAGGACCTTCAAATGCAGCTGGATGACAGCACACAACTGAACAGTGATCTG AAGGAGCAGGTGGCTGTGGCTGAGCGGCGCAACTCTCTTCTTCAGTCTGAACTAGAGGATCTAAGGTCC CTGCAAGAGCAGACAGAGCGTGGCCGCAGGCTGTCAGAAGAAGAGCTCCTGGAAGCAACAGAAAGAATC AATCTTTTCTATACCCAGAACACAAGCCTCCTCAGCCAGAAGAAGAAACTGGAGGCTGATGTTGCCCGG ATGCAGAAAGAAGCTGAAGAGGTGGTGCAGGAGTGTCAAAATGCAGAAGAGAAGGCCAAGAAGGCAGCC ATTGAGGCAGCAAACTTGTCAGAAGAACTGAAGAAGAAGCAAGACACCATTGCCCACTTGGAAAGGACA AGAGAAAATATGGAGCAGACAATTACAGACTTACAGAAAAGGCTGGCTGAAGCTGAACAGATGGCCCTG ATGGGGAGTAGAAAGCAAATCCAGAAACTAGAATCCAGGGTTCGTGAACTGGAAGGTGAACTGGAGGGT GAAATCCGTCGCAGTGCAGAGGCCCAGAGGGGAGCCCGCAGACTTGAGCGATGCATCAAAGAGCTGACC TATCAGGCAGAGGAAGACAAGAAGAATCTGAGCAGGATGCAAACTCAGATGGATAAACTTCAGCTAAAA GTGCAAAATTACAAGCAGCAAGTCGAGGTGGCGGAAACACAAGCCAATCAATACCTTTCCAAGTATAAG AAACAGCAACATGAGTTGAATGAAGTGAAGGAAAGGGCAGAGGTGGCAGAATCTCAAGTCAATAAACTC AAAATTAAAGCAAGAGAGTTTGGGAAAAAGGTTCAAGAAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_014981
Insert Size	5841 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014981.1
RefSeq Size	7074 bp
RefSeq ORF	5841 bp
Locus ID	22989
UniProt ID	Q9Y2K3
Protein Pathways	Tight junction, Viral myocarditis
MW	224.6 kDa
Gene Summary	Muscle contraction.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.