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CrkRS (CDK12) (NM_015083) Human Untagged Clone
CAT＃: SC317169
CDK12 (untagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRK7; CRKR; CRKRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317169 representing NM_015083.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGAGGAGCTTCTGGAACTTTGCAGCCG TCATCGGGAGGCGGCAGCTCTAACAGCAGAGAGCGTCACCGCTTGGTATCGAAGCACAAGCGGCATAAG TCCAAACACTCCAAAGACATGGGGTTGGTGACCCCCGAAGCAGCATCCCTGGGCACAGTTATCAAACCT TTGGTGGAGTATGATGATATCAGCTCTGATTCCGACACCTTCTCCGATGACATGGCCTTCAAACTAGAC CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCACAAACATCGTCACCACCAG CACAGGCGTTCCCGGGACTTACTAAAAGCTAAACAGACCGAAAAAGAAAAAAGCCAAGAAGTCTCCAGC AAGTCGGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGGAGACTGATGACTAT GGGAAGGCGCAGGTAGCCAAAAGCAGCAGCAAGGAATCCAGGTCATCCAAGCTCCACAAGGAGAAGACC AGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGTAAAAGTCATCGAAAAAGGGAAACACCC AAAAGTTACAAAACAGTGGACAGCCCAAAACGGAGATCCAGGAGCCCCCACAGGAAGTGGTCTGACAGC TCCAAACAAGATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTATGACCTTAGTCCCTCACGATCT CATACCTCGAGCAATTATGACTCCTACAAGAAAAGTCCTGGAAGTACCTCGAGAAGGCAGTCGGTCAGT CCCCCTTACAAGGAGCCTTCGGCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAG AGATCTGTCAGTCCCTATAGCAGGAGACGGTCGTCCAGCTACGAAAGAAGTGGCTCTTACAGCGGGCGA TCGCCCAGTCCCTATGGTCGAAGGCGGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGT CCACTCCCCAGTAGGAAATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTTCT CATAGTAAAAAGAAGAGATCCAGTTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCACTT AATTCCAGTCTGGGAGCTGAACTCAGTAGGAAAAAGAAGGAAAGAGCAGCTGCTGCTGCTGCAGCAAAG ATGGATGGAAAGGAGTCCAAGGGTTCACCTGTATTTTTGCCTAGAAAAGAGAACAGTTCAGTAGAGGCT AAGGATTCAGGTTTGGAGTCTAAAAAGTTACCCAGAAGTGTAAAATTGGAAAAATCTGCCCCAGATACT GAACTGGTGAATGTAACACATCTAAACACAGAGGTAAAAAATTCTTCAGATACAGGGAAAGTAAAGTTG GATGAGAACTCCGAGAAGCATCTTGTTAAAGATTTGAAAGCACAGGGAACAAGAGACTCTAAACCCATA GCACTGAAAGAGGAGATTGTTACTCCAAAGGAGACAGAAACATCAGAAAAGGAGACCCCTCCACCTCTT CCCACAATTGCTTCTCCCCCACCCCCTCTACCAACTACTACCCCTCCACCTCAGACACCCCCTTTGCCA CCTTTGCCTCCAATACCAGCTCTTCCACAGCAACCACCTCTGCCTCCTTCTCAGCCAGCATTTAGTCAG GTTCCTGCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGCTGTGTCCTCTCAG GCAAATTCTCAGCCCCCTGTACAGGTTTCTGTGAAGACTCAAGTATCTGTAACAGCTGCTATTCCACAC CTGAAAACTTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATGACATGGATAGT CCAAAAGAAACTCTTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTACTCACA GACCTTCCTCTCCCTCCAGAGCTCCCTGGTGGAGATCTGTCTCCCCCAGACTCTCCAGAACCAAAGGCA ATCACACCACCTCAGCAACCATATAAAAAGAGACCAAAAATTTGTTGTCCTCGTTATGGAGAAAGAAGA CAAACAGAAAGCGACTGGGGGAAACGCTGTGTGGACAAGTTTGACATTATTGGGATTATTGGAGAAGGA ACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGAACTAGTGGCTCTGAAGAAGGTGAGA CTAGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATC CACCGAAGTGTTGTTAACATGAAGGAAATTGTCACAGATAAACAAGATGCACTGGATTTCAAGAAGGAC AAAGGTGCCTTTTACCTTGTATTTGAGTATATGGACCATGACTTAATGGGACTGCTAGAATCTGGTTTG GTGCACTTTTCTGAGGACCATATCAAGTCGTTCATGAAACAGCTAATGGAAGGATTGGAATACTGTCAC AAAAAGAATTTCCTGCATCGGGATATTAAGTGTTCTAACATTTTGCTGAATAACAGTGGGCAAATCAAA CTAGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCCTTACACAAACAAAGTCATT ACTTTGTGGTACCGACCTCCAGAACTACTGCTAGGAGAGGAACGTTACACACCAGCCATAGATGTTTGG AGCTGTGGATGTATTCTTGGGGAACTATTCACAAAGAAGCCTATTTTTCAAGCCAATCTGGAACTGGCT CAGCTAGAACTGATCAGCCGACTTTGTGGTAGCCCTTGTCCAGCTGTGTGGCCTGATGTTATCAAACTG CCCTACTTCAACACCATGAAACCGAAGAAGCAATATCGAAGGCGTCTACGAGAAGAATTCTCTTTCATT CCTTCTGCAGCACTTGATTTATTGGACCACATGCTGACACTAGATCCTAGTAAGCGGTGCACAGCTGAA CAGACCCTACAGAGCGACTTCCTTAAAGATGTCGAACTCAGCAAAATGGCTCCTCCAGACCTCCCCCAC TGGCAGGATTGCCATGAGTTGTGGAGTAAGAAACGGCGACGTCAGCGACAAAGTGGTGTTGTAGTCGAA GAGCCACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTGAGCCTGTGAAGAAC AGCAGCCCAGCACCACCTCAGCCTGCTCCTGGCAAGGTGGAGTCTGGGGCTGGGGATGCAATAGGCCTT GCTGACATCACACAACAGCTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTGCAGAGCCAAACC GACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACATCCACTCCAACCCAGAGATGCAGCAGCAGCTG GAAGCCCTGAACCAATCCATCAGTGCCCTGACGGAAGCTACTTCCCAGCAGCAGGACTCAGAGACCATG GCCCCAGAGGAGTCTTTGAAGGAAGCACCCTCTGCCCCAGTGATCCTGCCTTCAGCAGAACAGACGACC CTTGAAGCTTCAAGCACACCAGCTGACATGCAGAATATATTGGCAGTTCTCTTGAGTCAGCTGATGAAA ACCCAAGAGCCAGCAGGCAGTCTGGAGGAAAACAACAGTGACAAGAACAGTGGGCCACAGGGGCCCCGA AGAACTCCCACAATGCCACAGGAGGAGGCAGCAGAGAAGAGGCCCCCTGAGCCCCCCGGACCTCCACCG CCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCAGCGCCCCCCAGGAGTTGAACCCAGCCGTG ACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCACCTGCCACATGAGCAC CAGGCCTTGAGACCAATGGAGTACTCCACCCGACCCCGTCCAAACAGGACTTATGGAAACACTGATGGG CCTGAAACAGGGTTCAGTGCCATTGACACTGATGAACGAAACTCTGGTCCAGCCTTGACAGAATCCTTG GTCCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTCTGAGCCACCTTGGGGAGTCCAGCAGTTAC CAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACCTCCGTTTTGCCAGGGTCCCCTTAGCGTTA CACCCGGTGGTCGGGCAACCATTCCTGAAGGCTGAGGGAAGCAGCAATTCTGTGGTACATGCAGAGACC AAATTGCAAAACTATGGGGAGCTGGGGCCAGGAACCACTGGGGCCAGCAGCTCAGGAGCAGGCCTTCAC TGGGGGGGCCCAACTCAGTCTTCTGCTTATGGAAAACTCTATCGGGGGCCTACAAGAGTCCCACCAAGA GGGGGAAGAGGGAGAGGAGTTCCTTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | AscI-MluI |
| ACCN | NM_015083 |
| Insert Size | 4446 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015083.2 |
| RefSeq Size | 8256 bp |
| RefSeq ORF | 4446 bp |
| Locus ID | 51755 |
| UniProt ID | Q9NYV4 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 163.2 kDa |
| Gene Summary | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226399 | CDK12 (Myc-DDK-tagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
CNY 10,136.00 |
|
| RC226399L1 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged |
CNY 12,536.00 |
|
| RC226399L2 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
CNY 16,720.00 |
|
| RC226399L3 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged |
CNY 12,536.00 |
|
| RC226399L4 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
CNY 12,536.00 |
|
| RG226399 | CDK12 (tGFP-tagged) - Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
CNY 16,340.00 |
