BPNT1 (NM_006085) Human Untagged Clone
CAT#: SC317461
BPNT1 (untagged)-Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1)
CNY 6,270.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HEL20; PIP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC317461 representing NM_006085.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTTCCAGTAACACTGTGTTGATGCGGTTGGTAGCCTCCGCATATTCTATTGCTCAAAAGGCAGGA ATGATAGTCAGACGTGTTATTGCTGAAGGAGACCTGGGTATTGTGGAGAAGACCTGTGCAACAGACCTG CAGACCAAAGCTGACCGATTGGCACAGATGAGCATATGTTCTTCATTGGCCCGGAAATTCCCCAAACTC ACAATTATAGGGGAAGAGGATCTGCCTTCTGAGGAAGTGGATCAAGAGCTGATTGAAGACAGTCAGTGG GAAGAAATACTGAAGCAACCATGCCCATCGCAGTACAGTGCTATTAAAGAAGAAGATCTCGTGGTCTGG GTTGATCCTCTGGATGGAACCAAGGAATATACCGAAGGTCTTCTTGACAATGTAACAGTTCTTATTGGA ATTGCTTATGAAGGAAAAGCCATAGCAGGAGTTATTAACCAGCCATATTACAACTATGAGGCAGGACCA GATGCTGTGTTGGGGAGGACAATCTGGGGAGTTTTAGGTTTAGGCGCCTTTGGGTTTCAGCTGAAAGAA GTCCCTGCTGGGAAACACATTATCACAACTACTCGATCCCATAGCAACAAGTTGGTTACTGACTGTGTT GCTGCTATGAACCCCGATGCTGTGCTGCGAGTAGGAGGAGCAGGAAATAAGATTATTCAGCTGATTGAA GGCAAAGCCTCTGCTTATGTATTTGCAAGTCCTGGTTGTAAGAAGTGGGATACTTGTGCTCCAGAAGTT ATTTTACATGCTGTGGGAGGCAAGTTAACCGATATCCATGGGAATGTTCTTCAGTACCACAAGGATGTG AAGCATATGAACTCTGCAGGAGTCCTGGCCACACTGAGGAATTATGACTACTATGCAAGCCGAGTTCCA GAATCTATTAAAAATGCACTTGTTCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_006085 |
Insert Size | 927 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006085.5 |
RefSeq Size | 2465 bp |
RefSeq ORF | 927 bp |
Locus ID | 10380 |
UniProt ID | O95861 |
Domains | inositol_P |
Protein Pathways | Sulfur metabolism |
MW | 33.4 kDa |
Gene Summary | BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216622 | BPNT1 (Myc-DDK-tagged)-Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1) |
CNY 2,400.00 |
|
RC216622L1 | Lenti ORF clone of Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC216622L2 | Lenti ORF clone of Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1), mGFP tagged |
CNY 5,890.00 |
|
RC216622L3 | Lenti ORF clone of Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216622L4 | Lenti ORF clone of Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1), mGFP tagged |
CNY 5,890.00 |
|
RG216622 | BPNT1 (tGFP-tagged) - Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1) |
CNY 4,000.00 |
|
SC116312 | BPNT1 (untagged)-Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1) |
CNY 2,400.00 |