TRBP (TARBP2) (NM_134324) Human Untagged Clone

CAT#: SC317539

TARBP2 (untagged)-Human TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 2



  "NM_134324" in other vectors (7)

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TARBP2 mouse monoclonal antibody, clone OTI7C1 (formerly 7C1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms LOQS; TRBP; TRBP1; TRBP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC317539 representing NM_134324.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGGCCGCCAACCCAGGCAAGACCCCGATCAGCCTTCTGCAGGAGTATGGGACCAGAATAGGGAAG
ACGCCTGTGTACGACCTTCTCAAAGCCGAGGGCCAAGCCCACCAGCCTAATTTCACCTTCCGGGTCACC
GTTGGCGACACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTG
GCCCTCAAACACCTCAAAGGGGGGAGCATGCTGGAGCCGGCCCTGGAGGACAGCAGTTCTTTTTCTCCC
CTAGACTCTTCACTGCCTGAGGACATTCCGGTTTTTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCT
GTAGTCCTAACCAGGAGCCCCCCCATGGAACTGCAGCCCCCTGTCTCCCCTCAGCAGTCTGAGTGCAAC
CCCGTTGGTGCTCTGCAGGAGCTGGTGGTGCAGAAAGGCTGGCGGTTGCCGGAGTACACAGTGACCCAG
GAGTCTGGGCCAGCCCACCGCAAAGAATTCACCATGACCTGTCGAGTGGAGCGTTTCATTGAGATTGGG
AGTGGCACTTCCAAAAAATTGGCAAAGCGGAATGCGGCGGCCAAAATGCTGCTTCGAGTGCACACGGTG
CCTCTGGATGCCCGGGATGGCAATGAGGTGGAGCCTGATGATGACCACTTCTCCATTGGTGTGGGCTCC
CGCCTGGATGGTCTTCGAAACCGGGGCCCAGGTTGCACCTGGGATTCTCTACGAAATTCAGTAGGAGAG
AAGATCCTGTCCCTCCGCAGTTGCTCCCTGGGCTCCCTGGGTGCCCTGGGCCCTGCCTGCTGCCGTGTC
CTCAGTGAGCTCTCTGAGGAGCAGGCCTTTCACGTCAGCTACCTGGATATTGAGGAGCTGAGCCTGAGT
GGACTCTGCCAGTGCCTGGTGGAACTGTCCACCCAGCCGGCCACTGTGTGTCATGGCTCTGCAACCACC
AGGGAGGCAGCCCGTGGTGAGGCTGCCCGCCGTGCCCTGCAGTACCTCAAGATCATGGCAGGCAGCAAG
TGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_134324
Insert Size 1038 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_134324.2
RefSeq Size 1488 bp
RefSeq ORF 1038 bp
Locus ID 6895
UniProt ID Q15633
Protein Families Stem cell - Pluripotency
MW 36.9 kDa
Gene Summary HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG codon for translation, as compared to variant 1. The encoded isoform (b) thus has a shorter N-terminus, as compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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