HuC (ELAVL3) (NM_032281) Human Untagged Clone

CAT#: SC317571

ELAVL3 (untagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 2



  "NM_032281" in other vectors (5)

CNY 5,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
ELAVL3 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HUC; HUCL; PLE21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC317571 representing NM_032281.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGCCCGGCCGGCCCGGCCCTGCCC
AACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGACAGCAAGACCAACCTCATCGTCAACTACCTG
CCCCAGAACATGACCCAGGATGAGTTCAAGAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAG
TTGGTTCGGGACAAGATCACAGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGAT
GCAGACAAAGCCATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCC
AGACCCAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCATGAGCCAG
AAAGAGATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCATCCTGGTGGACCAGGTC
ACAGGTGTCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAGGATTGAGGCCGAAGAGGCTATCAAA
GGACTGAATGGGCAGAAGCCGCTGGGCGCAGCTGAGCCCATCACAGTCAAGTTCGCGAACAACCCAAGT
CAGAAGACGGGGCAGGCGCTGCTCACCCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTA
CACCATCAGACCCAGCGTTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGGTTCTCG
CCGATCGCCATCGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGCGGCGGGCGCCGGC
TGGTGCATCTTCGTGTACAACCTGTCACCGGAGGCAGACGAGAGCGTGCTGTGGCAGCTGTTCGGGCCT
TTTGGGGCAGTCACCAACGTCAAGGTCATCCGTGATTTCACCACCAACAAGTGCAAGGGTTTCGGCTTC
GTGACCATGACCAACTATGACGAGGCGGCCATGGCCATCGCCAGCCTGAACGGCTATCGCCTGGGCGAG
CGCGTGCTGCAGGTCTCCTTCAAGACCAGCAAACAGCACAAGGCGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_032281
Insert Size 1083 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032281.2
RefSeq Size 4661 bp
RefSeq ORF 1083 bp
Locus ID 1995
UniProt ID Q14576
MW 38.9 kDa
Gene Summary A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, as compared to variant 1. It encodes isoform 2 which lacks an internal segment, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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