PRDM9 (NM_020227) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KMT8B; MEISETZ; MSBP3; PFM6; ZNF899
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_020227 edited ATGAGCCCTGAAAAGTCCCAAGAGGAGAGCCCAGAAGAAGACACAGAGAGAACAGAGCGG AAGCCCATGGTCAAAGATGCCTTCAAAGACATTTCCATATACTTCACCAAGGAAGAATGG GCAGAGATGGGAGACTGGGAGAAAACTCGCTATAGGAATGTGAAAAGGAACTATAATGCA CTGATTACTATAGGTCTCAGAGCCACTCGACCAGCTTTCATGTGTCACCGAAGGCAGGCC ATCAAACTCCAGGTGGATGACACAGAAGATTCTGATGAAGAATGGACCCCTAGGCAGCAA GTCAAACCTCCTTGGATGGCCTTAAGAGTGGAACAGCGTAAACACCAGAAGGGAATGCCC AAGGCGTCATTCAGTAATGAATCTAGTTTGAAAGAATTGTCAAGAACAGCAAATTTACTG AATGCAAGTGGCTCAGAGCAGGCTCAGAAACCAGTGTCCCCTTCTGGAGAAGCAAGTACC TCTGGACAGCACTCAAGACTAAAACTGGAACTCAGGAAGAAGGAGACTGAAAGAAAGATG TATAGCCTGCGAGAAAGAAAGGGTCATGCATACAAAGAGGTCAGCGAGCCGCAGGATGAT GATTACCTCTATTGTGAGATGTGTCAGAACTTCTTCATTGACAGCTGTGCTGCCCATGGG CCCCCTACATTTGTAAAGGACAGTGCAGTGGACAAGGGGCACCCCAACCGTTCAGCCCTC AGTCTGCCCCCAGGGCTGAGAATTGGGCCATCAGGCATCCCTCAGGCTGGGCTTGGAGTA TGGAATGAGGCATCTGATCTGCCGCTGGGTCTGCACTTTGGCCCTTATGAGGGCCGAATT ACAGAAGACGAAGAGGCAGCCAACAATGGATACTCCTGGCTGATCACCAAGGGGAGAAAC TGCTATGAGTATGTGGATGGAAAAGATAAATCCTGGGCCAACTGGATGAGGTATGTGAAC TGTGCCCGGGATGATGAAGAGCAGAACCTGGTGGCCTTCCAGTACCACAGGCAGATCTTC TATAGAACCTGCCGAGTCATTAGGCCAGGCTGTGAACTGCTGGTCTGGTATGGGGATGAA TACGGCCAGGAACTGGGCATCAAGTGGGGCAGCAAGTGGAAGAAAGAGCTCATGGCAGGG AGAGAACCAAAGCCAGAGATCCATCCATGTCCCTCATGCTGTCTGGCCTTTTCAAGTCAG AAATTTCTCAGTCAACATGTAGAACGCAATCACTCCTCTCAGAACTTCCCAGGACCATCT GCAAGAAAACTCCTCCAACCAGAGAATCCCTGCCCAGGGGATCAGAATCAGGAGCAGCAA TATCCAGATCCACACAGCCGTAATGACAAAACCAAAGGTCAAGAGATCAAAGAAAGGTCC AAACTCTTGAATAAAAGGACATGGCAGAGGGAGATTTCAAGGGCCTTTTCTAGCCCACCC AAAGGACAAATGGGGAGCTGTAGAGTGGGAAAAAGAATAATGGAAGAAGAGTCCAGAACA GGCCAGAAAGTGAATCCAGGGAACACAGGCAAATTATTTGTGGGGGTAGGAATCTCAAGA ATTGCAAAAGTCAAGTATGGAGAGTGTGGACAAGGTTTCAGTGTTAAATCAGATGTTATT ACACACCAAAGGACACATACAGGGGAGAAGCTCTACGTCTGCAGGGAGTGTGGGCGGGGC TTTAGCTGGAAGTCACACCTCCTCATTCACCAGAGGATACACACAGGGGAGAAGCCCTAT GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGG ACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCCGGCAG TCAGTCCTCCTCACTCACCAGAGGAGACACACAGGGGAGAAGCCCTATGTCTGCAGGGAG TGTGGGCGGGGCTTTAGCCGGCAGTCAGTCCTCCTCACTCACCAGAGGAGACACACAGGG GAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCTGGCAGTCAGTCCTCCTC ACTCACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC TTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGGACACACACAGGGGAGAAGCCCTAT GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCAATAAGTCACACCTCCTCAGACACCAGAGG ACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTCGCGATAAG TCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAG TGTGGGCGGGGCTTTAGAGATAAGTCAAACCTCCTCAGTCACCAGAGGACACACACAGGG GAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCAATAAGTCACACCTCCTC AGACACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC TTTCGCAATAAGTCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTAC GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCGATAGGTCAAGCCTCTGCTATCACCAGAGG ACACACACAGGGGAGAAGCCCTACGTCTGCAGGGAGGATGAGTAA
Restriction Sites	Please inquire
ACCN	NM_020227
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020227.2, NP_064612.2
RefSeq Size	3691 bp
RefSeq ORF	2685 bp
Locus ID	56979
UniProt ID	Q9NQV7
Gene Summary	The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (PRDM9 B allele, PMID:20044539) matches the reference genome sequence and encodes isoform PRDM9 B.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.