TAF4 (NM_003185) Human Untagged Clone

CAT#: SC318488

TAF4 (untagged)-Human TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa (TAF4)



  "NM_003185" in other vectors (2)

CNY 20,810.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-TAF4 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TAF(II)130; TAF(II)135; TAF2C; TAF2C1; TAF4A; TAFII-130; TAFII-135; TAFII130; TAFII135
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_003185, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGGCTCGGATCTGCTGGACGAGGTCTTCTTCAACAGCGAGGTGGACGAGAAA
GTGGTGAGCGACCTGGTGGGCTCGCTGGAGTCGCAGCTGGCGGCCAGCGCGGCCCACCAC
CACCACCTCGCGCCGCGCACGCCCGAGGTGCGGGCCGCGGCCGCCGGCGCGCTCGGGAAC
CATGTTGTGAGCGGCAGCCCGGCCGGAGCCGCGGGCGCAGGGCCGGCCGCCCCCGCCGAG
GGCGCGCCCGGAGCGGCGCCGGAGCCGCCCCCCGCAGGTAGAGCGCGGCCGGGGGGCGGG
GGGCCGCAGCGCCCGGGCCCCCCCTCACCGCGCCGCCCCCTTGTCCCCGCAGGGCCCGCG
CCGCCCGCCGCGAAGCTGAGGCCGCCGCCCGAGGGCAGCGCGGGGTCCTGCGCCCCGGTG
CCCGCCGCCGCCGCCGTCGCCGCGGGGCCCGAGCCCGCCCCCGCCGGCCCCGCCAAGCCC
GCCGGCCCCGCCGCGCTGGCCGCCCGCGCCGGCCCCGGCCCCGGGCCCGGCCCCGGCCCC
GGCCCCGGCCCTGGCCCTGGCAAGCCCGCCGGCCCCGGCGCCGCGCAAACTTTGAATGGG
AGCGCCGCGCTGCTGAACTCGCACCACGCCGCCGCACCTGCTGTCAGCCTGGTCAACAAC
GGGCCCGCCGCGCTGCTGCCGCTGCCCAAGCCCGCCGCCCCCGGCACTGTCATCCAGACG
CCCCCCTTCGTGGGCGCCGCCGCGCCCCCCGCGCCCGCCGCGCCCTCGCCCCCCGCCGCC
CCCGCGCCCGCCGCCCCCGCCGCCGCCCCGCCCCCGCCACCCCCCGCGCCCGCCACTCTG
GCCCGGCCGCCCGGCCACCCCGCCGGACCCCCGACCGCCGCGCCCGCCGTGCCGCCCCCC
GCCGCCGCCCAGAACGGGGGCAGCGCCGGGGCAGCCCCCGCCCCCGCCCCGGCCGCCGGG
GGCCCCGCGGGGGTCAGCGGCCAACCCGGGCCCGGCGCGGCGGCTGCGGCGCCGGCGCCG
GGGGTCAAGGCCGAGTCGCCCAAGAGGGTGGTGCAGGCGGCGCCCCCGGCGGCGCAGACC
CTGGCGGCCAGCGGCCCGGCCAGCACGGCGGCCAGCATGGTCATCGGGCCAACTATGCAA
GGGGCGCTGCCCAGCCCGGCCGCCGTCCCGCCGCCCGCCCCCGGGACCCCCACCGGGCTG
CCCAAAGGCGCGGCCGGCGCAGTGACCCAGAGCCTGTCCCGGACGCCCACGGCCACCACC
AGCGGGATTCGGGCCACCCTGACGCCCACCGTGCTGGCCCCCCGCTTGCCGCAGCCGCCT
CAGAACCCGACCAACATCCAGAACTTCCAGCTGCCCCCAGGAATGGTCCTCGTCCGAAGT
GAGAATGGGCAGTTGTTAATGATTCCTCAGCAGGCCTTGGCCCAGATGCAGGCGCAGGCC
CATGCCCAGCCTCAGACCACCATGGCGCCTCGCCCTGCCACCCCCACAAGTGCCCCTCCC
GTCCAGATCTCCACCGTACAGGCACCTGGAACACCTATCATTGCACGGCAGGTGACCCCA
ACTACCATAATTAAGCAAGTGTCTCAGGCCCAGACAACGGTGCAGCCCAGTGCAACCCTG
CAGCGCTCGCCCGGCGTCCAGCCTCAGCTCGTTCTGGGTGGCGCTGCCCAGACGGCTTCA
CTTGGGACGGCGACGGCTGTTCAGACGGGGACTCCTCAGCGCACGGTACCAGGGGCGACC
ACCACTTCCTCAGCTGCCACGGAAACTATGGAAAACGTGAAGAAATGTAAAAATTTCCTA
TCTACGTTAATAAAACTGGCTTCATCTGGCAAGCAGTCTACAGAGACAGCAGCTAATGTG
AAAGAGCTCGTGCAGAATTTACTGGATGGAAAAATAGAAGCAGAAGATTTCACAAGCAGG
TTATACCGAGAACTTAATTCTTCACCTCAACCTTACCTTGTGCCTTTCCTGAAGAGGAGC
TTACCCGCCTTGAGACAGCTGACCCCCGACTCCGCGGCCTTCATCCAGCAGAGCCAGCAG
CAGCCGCCACCGCCCACCTCGCAGGCCACCACTGCGCTCACGGCCGTGGTGCTGAGTAGC
TCGGTCCAGCGCACGGCCGGGAAGACGGCGGCCACCGTGACCAGTGCCCTCCAGCCCCCT
GTGCTCAGCCTCACGCAGCCCACGCAGGTCGGCGTCGGCAAGCAGGGGCAACCCACACCG
CTGGTCATCCAGCAGCCTCCGAAGCCAGGAGCCCTGATCCGGCCCCCGCAGGTGACGTTG
ACGCAGACACCCATGGTCGCCCTGCGGCAGCCTCACAACCGGATCATGCTCACCACGCCT
CAGCAGATCCAGCTGAACCCACTGCAGCCAGTCCCTGTGGTGAAACCCGCCGTGTTACCT
GGAACCAAAGCCCTTTCTGCTGTCTCGGCACAAGCAGCTGCTGCACAGAAAAATAAACTC
AAGGAGCCTGGGGGAGGTTCGTTTCGGGACGATGATGACATTAATGATGTTGCATCGATG
GCTGGAGTAAACTTGTCAGAAGAAAGTGCAAGAATATTAGCCACGAACTCTGAATTGGTG
GGCACGCTAACGCGGTCCTGTAAAGATGAAACCTTCCTCCTCCAAGCGCCTTTGCAGAGA
AGAATATTAGAAATAGGTAAAAAACATGGTATAACGGAATTACATCCAGATGTAGTAAGT
TATGTATCACATGCCACGCAACAAAGGCTACAGAATCTTGTAGAGAAAATATCAGAAACA
GCTCAGCAGAAGAACTTTTCTTACAAGGATGACGACAGATATGAGCAGGCGAGTGACGTC
CGGGCACAGCTCAAGTTTTTTGAACAGCTTGATCAAATCGAAAAGCAGAGGAAGGATGAG
CAGGAGCGGGAGATCCTGATGAGGGCAGCAAAGTCTCGGTCAAGACAAGAAGATCCAGAA
CAGTTAAGGCTGAAACAGAAGGCAAAGGAGATGCAGCAACAGGAACTGGCACAAATGAGA
CAGCGGGACGCCAACCTCACAGCACTAGCAGCGATCGGGCCCAGGAAAAAGAGGAAAGTG
GACTGTCCGGGGCCGGGCTCAGGAGCAGAGGGGTCGGGCCCCGGCTCAGTGGTCCCAGGC
AGCTCGGGTGTCGGAACCCCCAGACAGTTCACGCGACAAAGAATCACGCGGGTCAACCTC
AGGGACCTCATATTTTGTTTAGAAAATGAACGTGAGACAAGCCATTCACTGCTGCTCTAC
AAAGCATTCCTTAAG
Restriction Sites Please inquire     
ACCN NM_003185
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003185.3, NP_003176.2
RefSeq Size 4647 bp
RefSeq ORF 3258 bp
Locus ID 6874
UniProt ID O00268
Protein Pathways Basal transcription factors, Huntington's disease
Gene Summary Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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