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PAPP A2 (PAPPA2) (NM_020318) Human Untagged Clone
CAT＃: SC318671
PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 1
CNY 18,064.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PAPP-A2; PAPP-E; PAPPE; PLAC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318671 representing NM_020318.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGTGCTTAAAGATCCTAAGAATAAGCCTGGCGATTTTGGCTGGGTGGGCACTCTGTTCTGCCAAC TCTGAGCTGGGCTGGACACGCAAGAAATCCTTGGTTGAGAGGGAACACCTGAATCAGGTGCTGTTGGAA GGAGAACGTTGTTGGCTGGGGGCCAAGGTTCGAAGACCCAGAGCTTCTCCACAGCATCACCTCTTTGGA GTCTACCCCAGCAGGGCTGGGAACTACCTAAGGCCCTACCCCGTGGGGGAGCAAGAAATCCATCATACA GGACGCAGCAAACCAGACACTGAAGGAAATGCTGTGAGCCTTGTTCCCCCAGACCTGACTGAAAATCCA GCAGGACTGAGGGGTGCAGTTGAAGAGCCGGCTGCCCCATGGGTAGGGGATAGTCCTATTGGGCAATCT GAGCTGCTGGGAGATGATGACGCTTATCTCGGCAATCAAAGATCCAAGGAGTCTCTAGGTGAGGCCGGG ATTCAGAAAGGCTCAGCCATGGCTGCCACTACTACCACCGCCATTTTCACAACCCTGAACGAACCCAAA CCAGAGACCCAAAGGAGGGGCTGGGCCAAGTCCAGGCAGCGTCGCCAAGTGTGGAAGAGGCGGGCGGAA GATGGGCAGGGAGACTCCGGTATCTCTTCACATTTCCAACCTTGGCCCAAGCATTCCCTTAAACACAGG GTCAAAAAGAGTCCACCGGAGGAAAGCAACCAAAATGGTGGAGAGGGCTCCTACCGAGAAGCAGAGACC TTTAACTCCCAAGTAGGACTGCCCATCTTATACTTCTCTGGGAGGCGGGAGCGGCTGCTGCTGCGTCCA GAAGTGCTGGCTGAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTTAAACCGGAGGGAGGACAG AACAACCCAGCCATCATCGCAGGTGTGTTTGATAACTGCTCCCACACTGTCAGTGACAAAGGCTGGGCC CTGGGGATCCGCTCAGGGAAGGACAAGGGAAAGCGGGATGCTCGCTTCTTCTTCTCCCTCTGCACCGAC CGCGTGAAGAAAGCCACCATCTTGATTAGCCACAGTCGCTACCAACCAGGCACATGGACCCATGTGGCA GCCACTTACGATGGACGGCACATGGCCCTGTATGTGGATGGCACTCAGGTGGCTAGCAGTCTAGACCAG TCTGGTCCCCTGAACAGCCCCTTCATGGCATCTTGCCGCTCTTTGCTCCTGGGGGGAGACAGCTCTGAG GATGGGCACTATTTCCGTGGACACCTGGGCACACTGGTTTTCTGGTCGACCGCCCTGCCACAAAGCCAT TTTCAGCACAGTTCTCAGCATTCAAGTGGGGAGGAGGAAGCGACTGACTTGGTCCTGACAGCGAGCTTT GAGCCTGTGAACACAGAGTGGGTTCCCTTTAGAGATGAGAAGTACCCACGACTTGAGGTTCTCCAGGGC TTTGAGCCAGAGCCTGAGATTCTGTCGCCTTTGCAGCCCCCACTCTGTGGGCAAACAGTCTGTGACAAT GTGGAATTGATCTCCCAGTACAATGGATACTGGCCCCTTCGGGGAGAGAAGGTGATACGCTACCAGGTG GTGAACATCTGTGATGATGAGGGCCTAAACCCCATTGTGAGTGAGGAGCAGATTCGTCTGCAGCACGAG GCACTGAATGAGGCCTTCAGCCGCTACAACATCAGCTGGCAGCTGAGCGTCCACCAGGTCCACAATTCC ACCCTGCGACACCGGGTTGTGCTTGTGAACTGTGAGCCCAGCAAGATTGGCAATGACCATTGTGACCCC GAGTGTGAGCACCCACTCACAGGCTATGATGGGGGTGACTGCCGCCTGCAGGGCCGCTGCTACTCCTGG AACCGCAGGGATGGGCTCTGTCACGTGGAGTGTAACAACATGCTGAACGACTTTGACGACGGAGACTGC TGCGACCCCCAGGTGGCTGATGTGCGCAAGACCTGCTTTGACCCTGACTCACCCAAGAGGGCATACATG AGTGTGAAGGAGCTGAAGGAGGCCCTGCAGCTGAACAGTACTCACTTCCTCAACATCTACTTTGCCAGC TCAGTGCGGGAAGACCTTGCAGGTGCTGCCACCTGGCCTTGGGACAAGGACGCTGTCACTCACCTGGGT GGCATTGTCCTCAGCCCAGCATATTATGGGATGCCTGGCCACACCGACACCATGATCCATGAAGTGGGA CATGTTCTGGGACTCTACCATGTCTTTAAAGGAGTCAGTGAAAGAGAATCCTGCAATGACCCCTGCAAG GAGACAGTGCCATCCATGGAAACGGGAGACCTCTGTGCCGACACCGCCCCCACTCCCAAGAGTGAGCTG TGCCGGGAACCAGAGCCCACTAGTGACACCTGTGGCTTCACTCGCTTCCCAGGGGCTCCGTTCACCAAC TACATGAGCTACACGGATGATAACTGCACTGACAACTTCACTCCTAACCAAGTGGCCCGAATGCATTGC TATTTGGACCTAGTCTATCAGCAGTGGACTGAAAGCAGAAAGCCCACCCCCATCCCCATTCCACCTATG GTCATCGGACAGACCAACAAGTCCCTCACTATCCACTGGCTGCCTCCTATTAGTGGAGTTGTATATGAC AGGGCCTCAGGCAGCTTGTGTGGCGCTTGCACTGAAGATGGGACCTTTCGTCAGTATGTGCACACAGCT TCCTCCCGGCGGGTGTGTGACTCCTCAGGTTATTGGACCCCAGAGGAGGCTGTGGGGCCTCCTGATGTG GATCAGCCCTGCGAGCCAAGCTTACAGGCCTGGAGCCCTGAGGTCCACCTGTACCACATGAACATGACG GTCCCCTGCCCCACAGAAGGCTGTAGCTTGGAGCTGCTCTTCCAACACCCGGTCCAAGCCGACACCCTC ACCCTGTGGGTCACTTCCTTCTTCATGGAGTCCTCGCAGGTCCTCTTTGACACAGAGATCTTGCTGGAA AACAAGGAGTCAGTGCACCTGGGCCCCTTAGACACTTTCTGTGACATCCCACTCACCATCAAACTGCAC GTGGATGGGAAGGTGTCGGGGGTGAAAGTCTACACCTTTGATGAGAGGATAGAGATTGATGCAGCACTC CTGACTTCTCAGCCCCACAGTCCCTTGTGCTCTGGCTGCAGGCCTGTGAGGTACCAGGTTCTCCGCGAT CCCCCATTTGCCAGTGGTTTGCCCGTGGTGGTGACACATTCTCACAGGAAGTTCACGGACGTGGAGGTC ACACCTGGACAGATGTATCAGTACCAAGTTCTAGCTGAAGCTGGAGGAGAACTGGGAGAAGCTTCGCCT CCTCTGAACCACATTCATGGAGCTCCTTATTGTGGAGATGGGAAGGTGTCAGAGAGACTGGGAGAAGAG TGTGATGATGGAGACCTTGTGAGCGGAGATGGCTGCTCCAAGGTGTGTGAGCTGGAGGAAGGTTTCAAC TGTGTAGGAGAGCCAAGCCTTTGCTACATGTATGAGGGAGATGGCATATGTGAACCTTTTGAGAGAAAA ACCAGCATTGTAGACTGTGGCATCTACACTCCCAAAGGATACTTGGATCAATGGGCTACCCGGGCTTAC TCCTCTCATGAAGACAAGAAGAAGTGTCCTGTTTCCTTGGTAACTGGAGAACCTCATTCCCTAATTTGC ACATCATACCATCCAGATTTACCCAACCACCGTCCCCTAACTGGCTGGTTTCCCTGTGTTGCCAGTGAA AATGAAACTCAGGATGACAGGAGTGAACAGCCAGAAGGTAGCCTGAAGAAAGAGGATGAGGTTTGGCTC AAAGTGTGTTTCAATAGACCAGGAGAGGCCAGAGCAATTTTTATTTTTTTGACAACTGATGGCCTAGTT CCCGGAGAGCATCAGCAGCCGACAGTGACTCTCTACCTGACCGATGTCCGTGGAAGCAACCACTCTCTT GGAACCTATGGACTGTCATGCCAGCATAATCCACTGATTATCAATGTGACCCATCACCAGAATGTCCTT TTCCACCATACCACCTCAGTGCTGCTGAATTTCTCATCCCCACGGGTCGGCATCTCAGCTGTGGCTCTA AGGACATCCTCCCGCATTGGTCTTTCGGCTCCCAGTAACTGCATCTCAGAGGACGAGGGGCAGAATCAT CAGGGACAGAGCTGTATCCATCGGCCCTGTGGGAAGCAGGACAGCTGTCCGTCATTGCTGCTTGATCAT GCTGATGTGGTGAACTGTACCTCTATAGGCCCAGGTCTCATGAAGTGTGCTATCACTTGTCAAAGGGGA TTTGCCCTTCAGGCCAGCAGTGGGCAGTACATCAGGCCCATGCAGAAGGAAATTCTGCTCACATGTTCT TCTGGGCACTGGGACCAGAATGTGAGCTGCCTTCCCGTGGACTGCGGTGTTCCCGACCCGTCTTTGGTG AACTATGCAAACTTCTCCTGCTCAGAGGGAACCAAATTTCTGAAACGCTGCTCAATCTCTTGTGTCCCA CCAGCCAAGCTGCAAGGACTGAGCCCATGGCTGACATGTCTTGAAGATGGTCTCTGGTCTCTCCCTGAA GTCTACTGCAAGTTGGAGTGTGATGCTCCCCCTATTATTCTGAATGCCAACTTGCTCCTGCCTCACTGC CTCCAGGACAACCACGACGTGGGCACCATCTGCAAATATGAATGCAAACCAGGGTACTATGTGGCAGAA AGTGCAGAGGGTAAAGTCAGGAACAAGCTCCTGAAGATACAATGCCTGGAAGGTGGAATCTGGGAGCAA GGCAGCTGCATTCCTGTGGTGTGTGAGCCACCCCCTCCTGTGTTTGAAGGCATGTATGAATGTACCAAT GGCTTCAGCCTGGACAGCCAGTGTGTGCTCAACTGTAACCAGGAACGTGAAAAGCTTCCCATCCTCTGC ACTAAAGAGGGCCTGTGGACCCAGGAGTTTAAGTTGTGTGAGAATCTGCAAGGAGAATGCCCACCACCC CCCTCAGAGCTGAATTCTGTGGAGTACAAATGTGAACAAGGATATGGGATTGGTGCAGTGTGTTCCCCA TTGTGTGTAATCCCCCCCAGTGACCCCGTGATGCTACCTGAGAATATCACTGCTGACACTCTGGAGCAC TGGATGGAACCTGTCAAAGTCCAGAGCATTGTGTGCACTGGCCGGCGTCAATGGCACCCAGACCCCGTC TTAGTCCACTGCATCCAGTCATGTGAGCCCTTCCAAGCAGATGGTTGGTGTGACACTATCAACAACCGA GCCTACTGCCACTATGACGGGGGAGACTGCTGCTCTTCCACACTCTCCTCCAAGAAGGTCATTCCATTT GCTGCTGACTGTGACCTGGATGAGTGCACCTGCCGGGACCCCAAGGCAGAAGAAAATCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020318 |
| Insert Size | 5376 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020318.2 |
| RefSeq Size | 6940 bp |
| RefSeq ORF | 5376 bp |
| Locus ID | 60676 |
| UniProt ID | Q9BXP8 |
| Protein Families | Protease, Secreted Protein |
| MW | 198.5 kDa |
| Gene Summary | This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215960 | PAPPA2 (Myc-DDK-tagged)-Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 18,056.00 |
|
| RC215960L1 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 1, Myc-DDK-tagged |
CNY 20,456.00 |
|
| RC215960L3 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 1, Myc-DDK-tagged |
CNY 16,060.00 |
|
| RG215960 | PAPPA2 (tGFP-tagged) - Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 15,580.00 |
|
| SC125584 | PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 1 |
CNY 18,056.00 |
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