Ninein (NIN) (NM_020921) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SCKL7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318708 representing NM_020921. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGAGGTGGAGCAGGACCAGCATGAGGCCCGACTCAAGGAGCTGTTTGACAGTTTTGACACGACG GGCACAGGGTCCCTGGGGCAGGAGGAACTCACCGACCTTTGCCACATGTTGAGCTTGGAGGAGGTGGCC CCAGTGCTGCAGCAGACATTACTTCAGGACAACCTCTTGGGCAGGGTACATTTTGACCAATTTAAAGAA GCATTAATACTCATCTTGTCCAGAACTCTGTCAAATGAAGAACACTTTCAAGAACCAGACTGCTCACTA GAAGCTCAGCCCAAATATGTTAGAGGTGGGAAGCGTTACGGACGAAGGTCCTTGCCCGAGTTCCAAGAG TCCGTGGAGGAGTTTCCTGAAGTGACGGTGATTGAGCCACTGGATGAAGAAGCGCGGCCTTCACACATC CCAGCCGGTGACTGCAGTGAGCACTGGAAGACGCAACGCAGTGAGGAGTATGAAGCGGAAGGCCAGTTA AGGTTTTGGAACCCAGATGACTTGAATGCTTCACAGAGTGGATCTTCCCCTCCCCAAGACTGGATAGAA GAGAAACTGCAAGAAGTTTGTGAAGATTTGGGGATCACCCGTGATGGTCACCTGAACCGGAAGAAGCTG GTCTCCATCTGTGAGCAGTATGGTTTACAGAATGTGGATGGAGAGATGCTCGAGGAAGTATTCCATAAT CTTGATCCTGACGGTACAATGAGTGTAGAAGATTTTTTCTATGGTTTGTTTAAAAATGGAAAATCTCTT ACACCATCAGCATCTACTCCATATAGACAACTAAAAAGGCACCTTTCCATGCAGTCTTTCGATGAGAGT GGACGACGTACCACAACCTCATCAGCAATGACAAGTACCATTGGCTTTCGGGTCTTCTCCTGCCTGGAT GATGGGATGGGCCATGCATCTGTGGAGAGAATACTGGACACCTGGCAGGAAGAGGGCATTGAGAACAGC CAGGAGATCCTGAAGGCCTTGGATTTCAGCCTCGATGGAAACATCAATTTGACAGAATTAACACTGGCC CTTGAAAATGAACTTTTGGTTACCAAGAACAGCATTCACCAGGCGGCTCTGGCCAGCTTTAAGGCTGAA ATCCGGCATTTGTTGGAACGAGTTGATCAGGTGGTCAGAGAAAAAGAGAAGCTACGGTCAGATCTGGAC AAGGCCGAGAAGCTCAAGTCTTTAATGGCCTCGGAGGTGGATGATCACCATGCGGCCATAGAGCGGCGG AATGAGTACAACCTCAGGAAACTGGATGAAGAGTACAAGGAGCGAATAGCAGCCTTAAAAAATGAACTC CGAAAAGAGAGAGAGCAGATCCTGCAGCAGGCAGGCAAGCAGCGTTTAGAACTTGAACAGGAAATTGAA AAGGCAAAAACAGAAGAGAACTATATCCGGGACCGCCTTGCCCTCTCTTTAAAGGAAAACAGTCGTCTG GAAAATGAGCTTCTAGAAAATGCAGAGAAGTTGGCAGAATATGAGAATCTGACAAACAAACTTCAGAGA AATTTGGAAAATGTGTTAGCAGAAAAGTTTGGTGACCTCGATCCTAGCAGTGCTGAGTTCTTCCTGCAA GAAGAGAGACTGACACAGATGAGAAATGAATATGAGCGGCAGTGCAGGGTACTACAAGACCAAGTAGAT GAACTCCAGTCTGAGCTGGAAGAATATCGTGCACAAGGCAGAGTGCTCAGGCTTCCGTTGAAGAACTCA CCGTCAGAAGAAGTTGAGGCTAACAGCGGTGGCATTGAGCCCGAACACGGGCTCGGTTCTGAAGAATGC AATCCATTGAATATGAGCATTGAGGCAGAGCTGGTCATTGAACAGATGAAAGAACAACATCACAGGGAC ATATGTTGCCTCAGACTGGAGCTCGAAGATAAAGTGCGCCATTATGAAAAGCAGCTGGACGAAACCGTG GTCAGCTGCAAGAAGGCACAGGAGAACATGAAGCAAAGGCATGAGAACGAAACGCACACCTTAGAAAAA CAAATAAGTGACCTTAAAAATGAAATTGCTGAACTTCAGGGGCAAGCAGCAGTGCTCAAGGAGGCACAT CATGAGGCCACTTGCAGGCATGAGGAGGAGAAAAAACAACTGCAAGTGAAGCTTGAGGAGGAAAAGACT CACCTGCAGGAGAAGCTGAGGCTGCAACATGAGATGGAGCTCAAGGCTAGACTGACACAGGCTCAAGCA AGCTTTGAGCGGGAGAGGGAAGGCCTTCAGAGTAGCGCCTGGACAGAAGAGAAGGTGAGAGGCTTGACT CAGGAACTAGAGCAGTTTCACCAGGAGCAGCTGACAAGCCTGGTGGAGAAACACACTCTTGAGAAAGAG GAGTTAAGAAAAGAGCTCTTGGAAAAGCACCAAAGGGAGCTTCAGGAGGGAAGGGAAAAAATGGAAACA GAGTGTAATAGAAGAACCTCTCAAATAGAAGCCCAGTTTCAGTCTGATTGTCAGAAAGTCACTGAGAGG TGTGAAAGCGCTCTGCAAAGCCTGGAGGGGCGCTACCGCCAAGAGCTGAAGGACCTCCAGGAACAGCAG CGTGAGGAGAAATCCCAGTGGGAATTTGAGAAGGACGAGCTCACCCAGGAGTGTGCGGAAGCCCAGGAG CTGCTGAAAGAGACTCTTAAGAGAGAGAAAACAACTTCTCTGGTCCTGACCCAGGAGAGAGAGATGCTG GAGAAAACATACAAAGAACATTTGAACAGCATGGTCGTCGAGAGACAGCAGCTACTCCAAGACCTGGAA GACCTAAGAAATGTATCTGAAACCCAGCAAAGCCTGCTGTCTGACCAGATACTTGAGCTGAAGAGCAGT CACAAAAGGGAACTGAGGGAGCGTGAGGAGGTCCTGTGCCAGGCAGGGGCTTCGGAGCAGCTGGCCAGC CAGCGGCTGGAAAGACTAGAAATGGAACATGACCAGGAAAGGCAGGAAATGATGTCCAAGCTTCTAGCC ATGGAGAACATTCACAAAGCGACCTGTGAGACAGCAGATCGAGAAAGAGCCGAGATGAGCACAGAAATC TCCAGACTTCAGAGTAAAATAAAGGAAATGCAGCAGGCAACATCTCCTCTCTCAATGCTTCAGAGTGGT TGCCAGGTGATAGGAGAGGAGGAGGTGGAAGGAGATGGAGCCCTGTCCCTGCTTCAGCAAGGGGAGCAG CTGTTGGAAGAAAATGGGGACGTCCTCTTAAGCCTGCAGAGAGCTCATGAACAGGCAGTGAAGGAAAAT GTGAAAATGGCTACTGAAATTTCTAGATTGCAACAGAGGCTACAAAAGTTAGAGCCAGGGTTAGTAATG TCTTCTTGTTTGGATGAGCCAGCTACTGAGTTTTTTGGAAATACTGCGGAACAAACAGAGCCGTTTTTA CAGCAAAACCGAACGAAGCAAGTAGAAGGTGTGACCAGGCGGCATGTCCTAAGTGACCTGGAAGATGAT GAGGTCCGGGACCTGGGAAGTACAGGGACGAGCTCTGTTCAGAGACAGGAAGTCAAAATAGAGGAGTCT GAAGCTTCAGTAGAGGGTTTTTCTGAGCTTGAAAACAGTGAAGAGACCAGGACTGAATCCTGGGAGCTG AAGAATCAGATTAGTCAGCTTCAGGAACAGCTAATGATGTTATGTGCGGACTGTGATCGAGCTTCTGAA AAGAAACAGGACCTACTTTTTGATGTTTCTGTGCTAAAAAAGAAACTGAAGATGCTTGAGAGAATCCCT GAGGCTTCTCCCAAATATAAGCTGTTGTATGAAGATGTGAGCCGAGAAAATGACTGCCTTCAGGAAGAG CTGAGAATGATGGAGACACGCTACGATGAGGCACTAGAAAATAACAAAGAACTCACTGCAGAGGTTTTC AGGTTGCAGGATGAGCTGAAGAAAATGGAGGAAGTCACTGAAACATTCCTCAGCCTGGAAAAGAGTTAC GATGAGGTCAAAATAGAAAATGAGGAGCTGAATGTTCTGGTTTTGAGACTTCAAGGCAAGATTGAGAAG CTTCAGGAAAGCGTGGTCCAGCGGTGTGACTGCTGCTTATGGGAAGCCAGTTTAGAGAACCTGGAAATC GAACCTGATGGAAATATACTCCAGCTCAATCAGACACTGGAAGAGTGTGTGCCCAGGGTTAGGAGTGTA CATCATGTCATAGAGGAATGTAAGCAAGAAAACCAGTACCTTGAGGGGAACACACAGCTCTTGGAAAAA GTAAAAGCACATGAAATTGCCTGGTTACATGGAACAATTCAGACACATCAAGAAAGGCCAAGAGTACAG AATCAAGTTATACTGGAGGAAAACACTACTCTCCTAGGCTTTCAAGACAAACATTTTCAGCATCAGGCC ACCATAGCAGAGTTAGAACTGGAGAAAACAAAGTTACAGGAGCTGACTAGGAAGTTGAAGGAGAGAGTC ACTATTTTAGTTAAGCAAAAAGATGTACTTTCTCACGGAGAAAAGGAGGAAGAGCTGAAGGCAATGATG CATGACTTGCAGATCACGTGCAGTGAGATGCAGCAAAAAGTTGAACTTCTGAGATATGAATCTGAAAAG CTTCAACAGGAAAATTCTATTTTGAGAAATGAAATTACTACTTTAAATGAAGAAGATAGCATTTCTAAC CTGAAATTAGGGACATTAAATGGATCTCAGGAAGAAATGTGGCAAAAAACGGAAACTGTAAAACAAGAA AATGCTGCAGTTCAGAAGATGGTTGAAAATTTAAAGAAACAGATTTCAGAATTAAAAATCAAAAACCAA CAATTGGATTTGGAAAATACAGAACTTAGCCAAAAGAACTCTCAAAACCAGGAAAAACTGCAAGAACTT AATCAACGTCTAACAGAAATGCTATGCCAGAAGGAAAAAGAGCCAGGAAACAGTGCATTGGAGGAACGG GAACAAGAGAAGTTTAATCTGAAAGAAGAACTGGAACGTTGTAAAGTGCAGTCCTCCACTTTAGTGTCT TCTCTGGAGGCGGAGCTCTCTGAAGTTAAAATACAGACCCATATTGTGCAACAGGAAAACCACCTTCTC AAAGATGAACTGGAGAAAATGAAACAGCTGCACAGATGTCCCGATCTCTCTGACTTCCAGCAAAAAATC TCTAGTGTTCTAAGCTACAACGAAAAACTGCTGAAAGAAAAGGAAGCTCTGAGTGAGGAATTAAATAGC TGTGTCGATAAGTTGGCAAAATCAAGTCTTTTAGAGCATAGAATTGCGACGATGAAGCAGGAACAGAAA TCCTGGGAACATCAGAGTGCGAGCTTAAAGTCACAGCTGGTGGCTTCTCAGGAAAAGGTTCAGAATTTA GAAGACACCGTGCAGAATGTAAACCTGCAAATGTCCCGGATGAAATCTGACCTACGAGTGACTCAGCAG GAAAAGGAGGCTTTAAAACAAGAAGTGATGTCTTTACATAAGCAACTTCAGAATGCTGGTGGCAAGAGC TGGGCCCCAGAGATAGCTACTCATCCATCAGGGCTCCATAACCAGCAGAAAAGGCTGTCCTGGGACAAG TTGGATCATCTGATGAATGAGGAACAGCAGCTGCTTTGGCAAGAGAATGAGAGGCTCCAGACCATGGTA CAGAACACCAAAGCCGAACTCACGCACTCCCGGGAGAAGGTCCGTCAGTTGGAATCCAATCTTCTTCCC AAGCACCAAAAACATCTAAACCCATCAGGTACCATGAATCCCACAGAGCAAGAAAAATTGAGCTTAAAG AGAGAGTGTGATCAGTTTCAGAAAGAACAATCTCCTGCTAACAGGAAGGTCAGTCAGATGAATTCCCTT GAACAAGAATTAGAAACAATTCATTTGGAAAATGAAGGCCTGAAAAAGAAACAAGTAAAACTGGATGAG CAGCTCATGGAGATGCAGCACCTGAGGTCCACTGCGACGCCTAGCCCGTCCCCTCATGCTTGGGATTTG CAGCTGCTCCAGCAGCAAGCCTGTCCGATGGTGCCCAGGGAGCAGTTTCTGCAGCTTCAACGCCAGCTG CTGCAGGCAGAAAGGATAAACCAGCACCTGCAGGAGGAACTTGAAAACAGGACCTCCGAAACCAACACA CCACAGGGAAACCAGGAACAACTGGTAACTGTCATGGAGGAACGAATGATAGAAGTTGAACAGAAACTG AAACTAGTGAAAAGGCTTCTTCAAGAGAAAGTGAATCAGCTCAAAGAACAACTCTGCAAGAACACTAAG GCAGACGCAATGGTGAAGGACTTGTATGTTGAAAATGCCCAGTTGTTGAAAGCTCTGGAAGTGACTGAA CAGCGACAGAAAACAGCAGAGAAGAAAAATTACCTCCTGGAGGAGAAGATTGCCAGCCTCAGTAATATA GTTAGGAATCTGACACCAGCGCCATTGACTTCTACACCTCCTTTGAGGTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_020921
Insert Size	6402 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020921.3
RefSeq Size	10293 bp
RefSeq ORF	6402 bp
Locus ID	51199
UniProt ID	Q8N4C6
Domains	EFh
MW	248.2 kDa
Gene Summary	This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) is the longest transcript and it encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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