ZNF397 (NM_032347) Human Untagged Clone

CAT#: SC319130

ZNF397 (untagged)-Human zinc finger protein 397 (ZNF397), transcript variant 2



  "NM_032347" in other vectors (4)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ZNF397 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ZNF47; ZSCAN15
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_032347.1 GTGGCTTGCAGCTCGGGGTGGGTGGCTCATTTCCTGGCCGCTCCTGGGCTTCGCGGAAAG
AAGAGATTACTCACACTCCTTCGCAAGCACAGAACCAGTTGTACTGAGCTTTTTGCTAAG
CTGTTTCAGCCAAGAATGGCTGTGGAATCTGGAGTGATTTCAACCCTGATACCTCAGGAT
CCTCCGGAACAAGAACTAATACTAGTGAAAGTAGAAGATAACTTTTCCTGGGATGAGAAA
TTTAAGCAGAATGGGAGTACTCAATCCTGCCAAGAATTGTTTCGTCAGCAATTCAGAAAA
TTTTGCTACCAGGAGACACCTGGGCCCCGGGAGGCTCTGAGCCGACTCCAGGAACTTTGC
TATCAGTGGCTAATGCCAGAGTTGCACACAAAGGAGCAGATCTTAGAACTGCTGGTACTG
GAGCAGTTCCTGAGCATTCTGCCTGAGGAGCTGCAGATCTGGGTTCAGCAACATAATCCA
GAAAGCGGCGAGGAAGCTGTGACCCTGTTGGAGGATTTAGAGAGGGAGTTTGATGACCCA
GGGCAGCAGGTCCCAGCTAGTCCACAGGGACCAGCAGTGCCATGGAAGGATTTAACATGT
CTCAGAGCATCCCAAGAGTCAACAGACATCCACCTCCAGCCCTTAAAGACACAGCTGAAA
TCCTGGAAACCATGCCTTTCCCCTAAAAGTGATTGTGAGAACAGTGAAACAGCAACAAAA
GAGGGCATCTCAGAAGAAAAATCACAGGGACTCCCTCAGGAACCTTCATTTCGAGGAATT
AAGTTGTCCAGACCTCCCAAAGCTTCTTCAGCTATCCGTTGGGAATGTGTTTCTCCAGGA
AGTTTTCCCGGCGATATCATAGCTGCTGAGGCTACACATTCAACAATTTCTTGCTTTGCC
ATCAACACTTTGCCAGCCACCATCCTGCCATCTAAAAATGTGAATAGAAAGTATTTTTCC
TGAAGAACACATCGTCAATAAATCACCTGCACCCAAATCTCCATCTCAGGCGCTACTTCT
GGCAAGGCATATCATGAGGCTAGTGAAGCACCTACAAGAAACTTATAACTCAAGGTTGTA
AAAATGGCATGGTGACTCTTACAATCATATCACAGTGGGCCTCTCCAACAATTTAAACCT
CATGACCTAGGTGAAAGCTGTGAAAGGATCCACATTAATTCTTCAGAGGCAGGGCTAAGA
CTTTCAAGACTGAAAAGAGACTAGCATAAGGAGACTTAACAGGAACCCAGTCAAGGCCAT
TCATGGCCATGGGACCACGCATAAATGGAGAACGAGTAAATTTTGCTAAGTCCAAGGACA
TTGGAGAGATAGAGTCACTATTACTGATATGGTGACTGAGTCAACAACATACTGCCACTA
TGAAGTAATAAGAAAATAAAAAGTTAAAAAATGTAACAGAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_032347
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032347.1, NP_115723.1
RefSeq Size 1439 bp
RefSeq ORF 828 bp
Locus ID 84307
UniProt ID Q8NF99
Domains LER
Protein Families Transcription Factors
Gene Summary This gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc finger repeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, and different isoforms can repress or activate transcription in transfection studies. Multiple transcript variants encoding different isoforms have been found for this gene. Additional variants have been described, but their biological validity has not been determined. [provided by RefSeq, Oct 2009]
Transcript Variant: This variant (2) has an alternate 3' CDS and 3' UTR, as compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus that lacks the zinc finger domains, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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