PKC zeta (PRKCZ) (NM_002744) Human Untagged Clone

CAT#: SC319225

PRKCZ (untagged)-Human protein kinase C, zeta (PRKCZ), transcript variant 1



  "NM_002744" in other vectors (7)

CNY 6,624.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
Rabbit polyclonal PKC zeta (Thr410) antibody(Phospho-specific)
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PKC-ZETA; PKC2
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_002744.3 GCCCCGCGCGCCGCCGGAGTTCCGCGGAGTTGACCGGGTCGGCGCCGTCGGTCCTGAGCG
CTGCCTTCCGCGTTCCGCCGCGGCCCCACCTGGAGCCCCCGCCCCGCGCCATGGCCGGAG
CTCCCGGGGCGCAGCGCTGACGGCGGCGGGGGGAGCGCGCCATGCCCAGCAGGACCGGCC
CCAAGATGGAAGGGAGCGGCGGCCGCGTCCGCCTCAAGGCGCATTACGGGGGGGACATCT
TCATCACCAGCGTGGACGCCGCCACGACCTTCGAGGAGCTCTGTGAGGAAGTGAGAGACA
TGTGTCGTCTGCACCAGCAGCACCCGCTCACCCTCAAGTGGGTGGACAGCGAAGGTGACC
CTTGCACGGTGTCCTCCCAGATGGAGCTGGAAGAGGCTTTCCGCCTGGCCCGTCAGTGCA
GGGATGAAGGCCTCATCATTCATGTTTTCCCGAGCACCCCTGAGCAGCCTGGCCTGCCAT
GTCCGGGAGAAGACAAATCTATCTACCGCCGGGGAGCCAGAAGATGGAGGAAGCTGTACC
GTGCCAACGGCCACCTCTTCCAAGCCAAGCGCTTTAACAGGAGAGCGTACTGCGGTCAGT
GCAGCGAGAGGATATGGGGCCTCGCGAGGCAAGGCTACAGGTGCATCAACTGCAAACTGC
TGGTCCATAAGCGCTGCCACGGCCTCGTCCCGCTGACCTGCAGGAAGCATATGGATTCTG
TCATGCCTTCCCAAGAGCCTCCAGTAGACGACAAGAACGAGGACGCCGACCTTCCTTCCG
AGGAGACAGATGGAATTGCTTACATTTCCTCATCCCGGAAGCATGACAGCATTAAAGACG
ACTCGGAGGACCTTAAGCCAGTTATCGATGGGATGGATGGAATCAAAATCTCTCAGGGGC
TTGGGCTGCAGGACTTTGACCTAATCAGAGTCATCGGGCGCGGGAGCTACGCCAAGGTTC
TCCTGGTGCGGTTGAAGAAGAATGACCAAATTTACGCCATGAAAGTGGTGAAGAAAGAGC
TGGTGCATGATGACGAGGATATTGACTGGGTACAGACAGAGAAGCACGTGTTTGAGCAGG
CATCCAGCAACCCCTTCCTGGTCGGATTACACTCCTGCTTCCAGACGACAAGTCGGTTGT
TCCTGGTCATTGAGTACGTCAACGGCGGGGACCTGATGTTCCACATGCAGAGGCAGAGGA
AGCTCCCTGAGGAGCACGCCAGGTTCTACGCGGCCGAGATCTGCATCGCCCTCAACTTCC
TGCACGAGAGGGGGATCATCTACAGGGACCTGAAGCTGGACAACGTCCTCCTGGATGCGG
ACGGGCACATCAAGCTCACAGACTACGGCATGTGCAAGGAAGGCCTGGGCCCTGGTGACA
CAACGAGCACTTTCTGCGGAACCCCGAATTACATCGCCCCCGAAATCCTGCGGGGAGAGG
AGTACGGGTTCAGCGTGGACTGGTGGGCGCTGGGAGTCCTCATGTTTGAGATGATGGCCG
GGCGCTCCCCGTTCGACATCATCACCGACAACCCGGACATGAACACAGAGGACTACCTTT
TCCAAGTGATCCTGGAGAAGCCCATCCGGATCCCCCGGTTCCTGTCCGTCAAAGCCTCCC
ATGTTTTAAAAGGATTTTTAAATAAGGACCCCAAAGAGAGGCTCGGCTGCCGGCCACAGA
CTGGATTTTCTGACATCAAGTCCCACGCGTTCTTCCGCAGCATAGACTGGGACTTGCTGG
AGAAGAAGCAGGCGCTCCCTCCATTCCAGCCACAGATCACAGACGACTACGGTCTGGACA
ACTTTGACACACAGTTCACCAGCGAGCCCGTGCAGCTGACCCCAGACGATGAGGATGCCA
TAAAGAGGATCGACCAGTCAGAGTTCGAAGGCTTTGAGTATATCAACCCATTATTGCTGT
CCACCGAGGAGTCGGTGTGAGGCCGCGTGCGTCTCTGTCGTGGACACGCGTGATTGACCC
TTTAACTGTATCCTTAACCACCGCATATGCATGCCAGGCTGGGCACGGCTCCGAGGGCGG
CCAGGGACAGACGCTTGCGCCGAGACCGCAGAGGGAAGCGTCAGCGGGCGCTGCTGGGAG
CAGAACAGTCCCTCACACCTGGGCCCGGGCAGGCCAGCTTCGTGCTGGAGGAACTTGCTG
CTGTTCCTGCGTCGCGGCGGATCCGCGGGGACCCTGCCGAGGGGGCTGTCATGCGGTTTC
CAAGGTGCACATTTTCCACGGAAACAGAACTCGATGCACTGACCTGCTCCGCCAGGAAAG
TGAGCGTGTAGCGTCCTGAGGAATAAAATGTTCCGATGAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_002744
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002744.3, NP_002735.3
RefSeq Size 2326 bp
RefSeq ORF 1779 bp
Locus ID 5590
UniProt ID Q05513
Domains PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Endocytosis, Insulin signaling pathway, Tight junction, Type II diabetes mellitus
Gene Summary Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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