ZNF274 (NM_016324) Human Untagged Clone
CAT#: SC320278
ZNF274 (untagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b
CNY 4,088.00
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HFB101; ZF2; ZKSCAN19; ZSCAN51 |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_016324.2
AGAGACACATTGAGAAGGAGGAAACTATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTG
AACCAGTGACCTTTGAAGATGTAACACTGGGTTTTACCCCGGAAGAGTGGGGACTGCTGG ACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAACTACAGGAACCTGGTCT CAGTGGAACATCAGCTTTCCAAACCAGATGTGGTATCTCAGTTAGAGGAGGCAGAAGATT TCTGGCCAGTGGAGAGAGGAATTCCTCAAGACACCATTCCAGAGTATCCTGAGCTCCAGC TGGACCCTAAATTGGATCCTCTTCCTGCTGAGAGTCCCCTAATGAACATTGAGGTTGTTG AGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCCAGGCCCTAT ATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGATCCAACAGCAGGGCA AGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACA TGACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGC CTAAGGCACGCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTG CACTGCCTGTGAAGCTCCGGACATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGG TGGTGGCCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAGGAAGTACTTCCTGCAGGAC AACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGGAGAAGCAGG AGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATG TGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGT ACCGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACAC TGGAAAATAAAGAGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCC TGAAAGTACAAGAATCCTCAAGGGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGC AGGGTGGGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAGATGGAGTCTGCTCAGGAAA AAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCCAGGCAAACAGTGGTGCTC TTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGCAAACAGTG GCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCA AACGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGA GCTGTGAAAGGCAAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTA CTAAACAGATTACGTTTATAAGAATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAAT GTGGTAAAATATTCCGGAACCCAAGATACTTTTCTGTGCATAAGAAAATCCATACCGGAG AGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTTCCCTCACAC AGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGGGAGGACCT TCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACA AGTGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGA CTCACACCGGGGAGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCT CACACCTTATTGGGCACCAGAGAACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTA CCTCATAGCTCTCAAGCCAGTTGAAGAAACCTTGCCTTTTCAGCTTGACCCTGCAATATA ACATGCACAGGCCTGCTTGTGAATCAGGACTGAATGTGAAAGGGAAGTATTGAGTGAGGA CATTCCCAAAACCAAAGGACAACTGAGGAGACTGCCCAGCACATAATGAATAAATAAGAA AATGAGTGAGGAGTTATTAACATCATTTGGAAAAAAGATTTCCCATTCACTTGATATTGT TTGTTCACTCATTTAGTCATTAAAAGTGAGATTAATAAAATCTGAAAATGTTATATAATA ACTTTAAAAAGCCAGGTAATTAATAATCTGCACTGATATTACATCCACAGTACCACAGTA TTTATGTGTATGAATTAAGGATTAAAAGATAATGTGGATAAATAAACTATTGATCTATGA AAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_016324 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016324.2, NP_057408.2 |
RefSeq Size | 2538 bp |
RefSeq ORF | 1647 bp |
Locus ID | 10782 |
UniProt ID | Q96GC6 |
Domains | KRAB, LER, zf-C2H2 |
Protein Families | Transcription Factors |
Protein Pathways | Neurotrophin signaling pathway |
Gene Summary | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (ZNF274b) lacks three consecutive exons in the 5' region, which results in a downstream translation start codon, compared to variant ZNF274c. The resulting isoform (b) has a shorter N-terminus and contains only one KRAB A domain, compared to isoform c. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200209 | ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 5,510.00 |
|
RC200209L3 | Lenti-ORF clone of ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 7,410.00 |
|
RC200209L4 | Lenti-ORF clone of ZNF274 (mGFP-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 7,410.00 |
|
RG200209 | ZNF274 (tGFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 6,080.00 |