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WASP (WAS) (NM_000377) Human Untagged Clone
CAT＃: SC321241
WAS (untagged)-Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS)
CNY 3,744.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IMD2; SCNX; THC; THC1; WASP; WASPA |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000377.1
GCCAGAGAAGACAAGGGCAGAAAGCACCATGAGTGGGGGCCCAATGGGAGGAAGGCCCGG
GGGCCGAGGAGCACCAGCGGTTCAGCAGAACATACCCTCCACCCTCCTCCAGGACCACGA GAACCAGCGACTCTTTGAGATGCTTGGACGAAAATGCTTGACGCTGGCCACTGCAGTTGT TCAGCTGTACCTGGCGCTGCCCCCTGGAGCTGAGCACTGGACCAAGGAGCATTGTGGGGC TGTGTGCTTCGTGAAGGATAACCCCCAGAAGTCCTACTTCATCCGCCTTTACGGCCTTCA GGCTGGTCGGCTGCTCTGGGAACAGGAGCTGTACTCACAGCTTGTCTACTCCACCCCCAC CCCCTTCTTCCACACCTTCGCTGGAGATGACTGCCAAGCGGGGCTGAACTTTGCAGACGA GGACGAGGCCCAGGCCTTCCGGGCCCTCGTGCAGGAGAAGATACAAAAAAGGAATCAGAG GCAAAGTGGAGACAGACGCCAGCTACCCCCACCACCAACACCAGCCAATGAAGAGAGAAG AGGAGGGCTCCCACCCCTGCCCCTGCATCCAGGTGGAGACCAAGGAGGCCCTCCAGTGGG TCCGCTCTCCCTGGGGCTGGCGACAGTGGACATCCAGAACCCTGACATCACGAGTTCACG ATACCGTGGGCTCCCAGCACCTGGACCTAGCCCAGCTGATAAGAAACGCTCAGGGAAGAA GAAGATCAGCAAAGCTGATATTGGTGCACCCAGTGGATTCAAGCATGTCAGCCACGTGGG GTGGGACCCCCAGAATGGATTTGACGTGAACAACCTCGACCCAGATCTGCGGAGTCTGTT CTCCAGGGCAGGAATCAGCGAGGCCCAGCTCACCGACGCCGAGACCTCTAAACTTATCTA CGACTTCATTGAGGACCAGGGTGGGCTGGAGGCTGTGCGGCAGGAGATGAGGCGCCAGGA GCCACTTCCGCCGCCCCCACCGCCATCTCGAGGAGGGAACCAGCTCCCCCGGCCCCCTAT TGTGGGGGGTAACAAGGGTCGTTCTGGTCCACTGCCCCCTGTACCTTTGGGGATTGCCCC ACCCCCACCAACACCCCGGGGACCCCCACCCCCAGGCCGAGGGGGCCCTCCACCACCACC CCCTCCAGCTACTGGACGTTCTGGACCACTGCCCCCTCCACCCCCTGGAGCTGGTGGGCC ACCCATGCCACCACCACCGCCACCACCGCCACCGCCGCCCAGCTCCGGGAATGGACCAGC CCCTCCCCCACTCCCTCCTGCTCTGGTGCCTGCCGGGGGCCTGGCCCCTGGTGGGGGTCG GGGAGCGCTTTTGGATCAAATCCGGCAGGGAATTCAGCTGAACAAGACCCCTGGGGCCCC AGAGAGCTCAGCGCTGCAGCCACCACCTCAGAGCTCAGAGGGACTGGTGGGGGCCCTGAT GCACGTGATGCAGAAGAGAAGCAGAGCCATCCACTCCTCCGACGAAGGGGAGGACCAGGC TGGCGATGAAGATGAAGATGATGAATGGGATGACTGAGTGGCTGAGTTACTTGCTGCCCT GTGCTCCTCCCCGCAGGACATGGCTCCCCCTCCACCTGCTCTGTGCCCACCCTCCACTCT CCTCTTCCAGGCCCCCAACCCCCCATTTCTTCCCCACCAACCCCTCCAATGCTGTTATCC CTGCCTGGTCCTCACACTCACCCAACAATCCCAAGGCCCTTTTTATACAAAAATTCTCAG TTCTCTTCACTCAAGGATTTTTAAAGAAAAATAAAAGAATTGTCTTTCTGTCTCTCAAAA AAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000377 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000377.1, NP_000368.1 |
| RefSeq Size | 1806 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 7454 |
| UniProt ID | P42768 |
| Domains | PBD, WH1, WH2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton |
| Gene Summary | The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203457 | WAS (Myc-DDK-tagged)-Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS) |
CNY 3,736.00 |
|
| RC203457L1 | Lenti ORF clone of Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS), Myc-DDK-tagged |
CNY 6,136.00 |
|
| RC203457L2 | Lenti ORF clone of Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS), mGFP tagged |
CNY 5,890.00 |
|
| RC203457L3 | Lenti ORF clone of Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS), Myc-DDK-tagged |
CNY 6,136.00 |
|
| RC203457L4 | Lenti ORF clone of Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS), mGFP tagged |
CNY 6,136.00 |
|
| RG203457 | WAS (tGFP-tagged) - Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS) |
CNY 4,370.00 |
