TFIIF (GTF2F2) (NM_004128) Human Untagged Clone

CAT#: SC321338

GTF2F2 (untagged)-Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)



  "NM_004128" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
GTF2F2 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BTF4; RAP30; TF2F2; TFIIF
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_004128.1 AAGGAACGCCGGCTCTTCGCCTCTCAGCGCGGCTTGTCCTTTGTTCCGGACGCCCGCTCC
TCAGCCCTGCGGCTCCTGGGGTCGCTGCTGCATCCCGCACGCCTCCACCGGCTGCAGACC
CATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCT
AGTCAAGGTTCCTAAATATTTGTCACAGCAATGGGCTAAAGCTTCTGGAAGAGGTGAAGT
TGGGAAACTGCGGATTGCCAAGACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGA
GGATCTTGCAAATATTCATGATATTGGTGGAAAACCAGCTTCAGTCAGTGCTCCTAGAGA
ACATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAACAGTATTTACTGAGAGCTC
ATCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCGACCAGCTGC
CAGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAG
GCTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCA
ATACAATATCGAATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAA
ACAACATGTTTTAGACATGCTATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAA
GGACTTGGTGGACATCACAAAACAACCTGTGGTGTACCTGAAGGAAATCTTAAAAGAAAT
TGGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGGAGCTGAAGCCAGAGTACAG
ACACTATCAAGGAGAAGAAAAGAGTGACTAAGAAGACTCCTAGCCAGCATGCTAGTGAAA
CGACTAGCAGCGATGCTATGCAAAAGGCGCTGATACTGGAAGGCTTGAACACCGTATGTT
AATAGGGGTTAAGTGACAGTACTTTGATTTCTCTCGGTAAATTTTTTAAACCTGTAATTC
TTGTAAAGTTTCTTAACTGTTTTTTTGAGGAGAAAGAACAGATTTATTTATAGACTTAAC
TTGTATTAAACCAGATTATTCACAGTAGGAATAGGGGTTGGAGGATTAAGAGGGTTTTTC
TTAAATATTAGCTTTTAATGTGTACAATTAGGAAATTTTTTTAAGTGAGGACTCTCTACC
CTTGCCGTATCTAAGGAGCTGAGGTAATACAGATCCAAGGAGAATTGTATAGCAAGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_004128
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004128.1, NP_004119.1
RefSeq Size 1408 bp
RefSeq ORF 750 bp
Locus ID 2963
UniProt ID P13984
Protein Families Transcription Factors
Protein Pathways Basal transcription factors
Gene Summary TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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