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NANS (NM_018946) Human Untagged Clone
CAT＃: SC321367
NANS (untagged)-Human N-acetylneuraminic acid synthase (NANS)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-100; SAS; SEMDCG; SEMDG |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_018946.2
CACAGAACAGAGTAGAGGCGGCGGCGGCGGCGGCCGGACCCAGACTGGTAGTGAGGCGTT
GGACCCCGAGCCGCTGCAATGCCGCTGGAGCTGGAGCTGTGTCCCGGGCGCTGGGTGGGC GGGCAACACCCGTGCTTCATCATTGCCGAGATCGGCCAGAACCACCAGGGCGACCTGGAT GTAGCCAAGCGCATGATCCGCATGGCCAAGGAGTGTGGGGCTGATTGTGCCAAGTTCCAG AAGAGTGAGCTAGAATTCAAGTTTAATCGGAAAGCCTTGGAGAGGCCATACACCTCGAAG CATTCCTGGGGGAAGACGTACGGGGAGCACAAACGACATCTGGAGTTCAGCCATGACCAG TACAGGGAGCTGCAGAGGTACGCCGAGGAGGTTGGGATCTTCTTCACTGCCTCTGGCATG GATGAGATGGCAGTTGAATTCCTGCATGAACTGAATGTTCCATTTTTCAAAGTTGGATCT GGAGACACTAATAATTTTCCTTATCTGGAAAAGACAGCCAAAAAAGGTCGCCCAATGGTG ATCTCCAGTGGGATGCAGTCAATGGACACCATGAAGCAAGTTTATCAGATCGTGAAGCCC CTCAACCCCAACTTCTGCTTCTTGCAGTGTACCAGCGCATACCCGCTCCAGCCTGAGGAC GTCAACCTGCGGGTCATCTCGGAATATCAGAAGCTCTTTCCTGACATTCCCATAGGGTAT TCTGGGCATGAAACAGGCATAGCGATATCTGTGGCCGCAGTGGCTCTGGGGGCCAAGGTG TTGGAACGTCACATAACTTTGGACAAGACCTGGAAGGGGAGTGACCACTCGGCCTCGCTG GAGCCTGGAGAACTGGCCGAGCTGGTGCGGTCAGTGCGTCTTGTGGAGCGTGCCCTGGGC TCCCCAACCAAGCAGCTGCTGCCCTGTGAGATGGCCTGCAATGAGAAGCTGGGCAAGTCT GTGGTGGCCAAAGTGAAAATTCCGGAAGGCACCATTCTAACAATGGACATGCTCACCGTG AAGGTGGGTGAGCCCAAAGGCTATCCTCCTGAAGACATCTTTAATCTAGTGGGCAAGAAG GTCCTGGTCACTGTTGAAGAGGATGACACCATCATGGAAGAATTGGTAGATAATCATGGC AAAAAAATCAAGTCTTAAAAATAAAGTGCCATTCTCTGAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_018946 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018946.2, NP_061819.2 |
| RefSeq Size | 1170 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 54187 |
| UniProt ID | Q9NR45 |
| Domains | NeuB, Antifreeze |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200123 | NANS (Myc-DDK-tagged)-Human N-acetylneuraminic acid synthase (NANS) |
CNY 3,656.00 |
|
| RC200123L3 | Lenti ORF clone of Human N-acetylneuraminic acid synthase (NANS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200123L4 | Lenti ORF clone of Human N-acetylneuraminic acid synthase (NANS), mGFP tagged |
CNY 5,890.00 |
|
| RG200123 | NANS (tGFP-tagged) - Human N-acetylneuraminic acid synthase (NANS) |
CNY 5,256.00 |
