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TRMT61B (NM_017910) Human Untagged Clone
CAT＃: SC321424
TRMT61B (untagged)-Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017910 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017910.2, NP_060380.2 |
| RefSeq Size | 1846 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 55006 |
| UniProt ID | Q9BVS5 |
| Protein Families | Druggable Genome |
| Gene Summary | Methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (PubMed:23097428). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function (PubMed:27631568). In addition to tRNA N(1)-methyltransferase activity, also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA, leading to interfere with mitochondrial translation (PubMed:29107537).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201499 | TRMT61B (Myc-DDK-tagged)-Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B) |
CNY 3,656.00 |
|
| RC201499L3 | Lenti ORF clone of Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201499L4 | Lenti ORF clone of Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B), mGFP tagged |
CNY 5,890.00 |
|
| RG201499 | TRMT61B (tGFP-tagged) - Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B) |
CNY 5,256.00 |
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