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PDK1 (NM_002610) Human Untagged Clone
CAT＃: SC321678
PDK1 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein
CNY 5,488.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_002610.3
GAAACCTGGCGTACTGGCTGTGGCTTCTCTAGCGGGACTCGGCATGAGGCTGGCGCGGCT
GCTTCGCGGAGCCGCCTTGGCCGGCCCGGGCCCGGGGCTGCGCGCCGCCGGCTTCAGCCG CAGCTTCAGCTCGGACTCGGGCTCCAGCCCGGCGTCCGAGCGCGGCGTTCCGGGCCAGGT GGACTTCTACGCGCGCTTCTCGCCGTCCCCGCTCTCCATGAAGCAGTTCCTGGACTTCGG ATCAGTGAATGCTTGTGAAAAGACCTCATTTATGTTTCTGCGGCAAGAGTTGCCTGTCAG ACTGGCAAATATAATGAAAGAAATAAGTCTCCTTCCAGATAATCTTCTCAGGACACCATC CGTTCAATTGGTACAAAGCTGGTATATCCAGAGTCTTCAGGAGCTTCTTGATTTTAAGGA CAAAAGTGCTGAGGATGCTAAAGCTATTTATGACTTTACAGATACTGTGATACGGATCAG AAACCGACACAATGATGTCATTCCCACAATGGCCCAGGGTGTGATTGAATACAAGGAGAG CTTTGGGGTGGATCCTGTCACCAGCCAGAATGTTCAGTACTTTTTGGATCGATTCTACAT GAGTCGCATTTCAATTAGAATGTTACTCAATCAGCACTCTTTATTGTTTGGTGGAAAAGG CAAAGGAAGTCCATCTCATCGAAAACACATTGGAAGCATAAATCCAAACTGCAATGTACT TGAAGTTATTAAAGATGGCTATGAAAATGCTAGGCGTCTGTGTGATTTGTATTATATTAA CTCTCCCGAACTAGAACTTGAAGAACTAAATGCAAAATCACCAGGACAGCCAATACAAGT GGTTTATGTACCATCCCATCTCTATCACATGGTGTTTGAACTTTTCAAGAATGCAATGAG AGCCACTATGGAACACCATGCCAACAGAGGTGTTTACCCCCCTATTCAAGTTCATGTCAC GCTGGGTAATGAGGATTTGACTGTGAAGATGAGTGACCGAGGAGGTGGCGTTCCTTTGAG GAAAATTGACAGACTTTTCAACTACATGTATTCAACTGCACCAAGACCTCGTGTTGAGAC CTCCCGCGCAGTGCCTCTGGCTGGTTTTGGTTATGGATTGCCCATATCACGTCTTTACGC ACAATACTTCCAAGGAGACCTGAAGCTGTATTCCCTAGAGGGTTACGGGACAGATGCAGT TATCTACATTAAGGCTCTGTCAACAGACTCAATAGAAAGACTCCCAGTGTATAACAAAGC TGCCTGGAAGCATTACAACACCAACCACGAGGCTGATGACTGGTGCGTCCCCAGCAGAGA ACCCAAAGACATGACGACGTTCCGCAGTGCCTAGACACACTTGGGACATCGGAAAATCCA AATGTGGCTTTTGTATTAAATTTGGAAGTGTGGCCCAGAGTTGCTCAGAATTGGAGCAGA GCCTGAGACGTATCTGCAGATCCTGTCATCAGCTGGCAAGTCCAGGAGACTGTGTCATTT AGAGACTGTGTTGTTAGTTATCCCTCAACATCTTCTAAGGTGGCAGGAAATAATATTGGA AATAACATTTTAAAGTAAAAATTTTAAAGTTTAAAGAAGAGTTTTGCCACTTAAACAGGG GAGCTTTGTCTGGAAAATACACTGAGTTGAAACACTTCATCCTTGGAAGGATTATATAAG ATGAACAGTTGTGATAAATGTGTAGATTAGAGGGATGTGAATGGGCAGTTAGTCCAGTGC CCTCATTTAAGAGGCCAAGATCCTGATTCAGAGGAGGCATCCTTTGCCCAGAGCTGCTTA GCTAATCTGACCAAATGTTGGGAAAAATGTCTCACCTAACCCACTATTCCTTAATTATGG ATTTTGTGAAAAACAATAGAACATGTTAATGAGTAATTTATATTAGTTCGATGTATTACA ATTTTTTAGCTTTAAATTACAGTTTTCTTATAATGTTGAAATGTTTTAGAATCCTTTGAA TCTAAGTATTTGTTTCCTAAATGAAACATTTGTACAACATTTGATGTTTTTACTTATGAA ATATTCTCCTCCCCCAAGAAAATTTAAACTTTTTCTCTCTATTTAAAAGCTAAGAAATGT TTTAAAGGAAAAATGAAATTATCTTCCTTTAGCTTATTTTTAAGGTAAAACAGCTTTTTA CTCTGTTATTGTGGTAATGGACAGAATATTACATACAAAAATATTCTGGGAGAGCTTTTT CCTAGTTGGTTTTAAATCATTGTGCCACCTGAAAGGTTTTTAGATTTTATAGGAGCTAAT TTGTCCACCAGCATTAATGTAACACAGTGTAGTTATGAAAATATATTGAAGGACAGGAAG TGGACACGAAGTGATTTTTGTAACCTGAGCAGTTAATGAATGTGCCAACATTTTCTAGGA AGGGACAGCAAGAATATTCTGCTCTGTAGTTAAAATACTGGCTGGCTTTTGATGTCTTCA TGCTTAATTGTGATCACTTTCTTGCACTGTGATGTTTTTACGTGAATATGTTGAAGTAGA AGTCTACCATATTATTTTATAAAATGTTTTCTGTATGGCAATAAACTGAAAACATGGATC AACCCTTCTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_002610 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002610.3, NP_002601.1 |
| RefSeq Size | 4576 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 5163 |
| UniProt ID | Q15118 |
| Domains | HATPase_c |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Fc epsilon RI signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway |
| Gene Summary | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Four Key Steps Control Glycolytic Flux in Mammalian Cells
,Tanner, LB;Goglia, AG;Wei, MH;Sehgal, T;Parsons, LR;Park, JO;White, E;Toettcher, JE;Rabinowitz, JD;,
Cell Syst
,PubMed ID 29960885
[PDK1]
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Mitochondrial complex II is a source of the reserve respiratory capacity that is regulated by metabolic sensors and promotes cell survival
,Pfleger, J;He, M;Abdellatif, M;,
Cell Death Dis
,PubMed ID 26225774
[PDK1]
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Overcoming 5-Fu resistance in human non-small cell lung cancer cells by the combination of 5-Fu and cisplatin through the inhibition of glucose metabolism
,Zhao, JG;Ren, KM;Tang, J;,
Tumour Biol.
,PubMed ID 25260882
[PDK1]
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Overexpression of Pyruvate Dehydrogenase Kinase 1 and Lactate Dehydrogenase A in Nerve Cells Confers Resistance to Amyloid ß and Other Toxins by Decreasing Mitochondrial Respiration and Reactive Oxygen Species Production
,Jordan T. Newington, Tim Rappon, Shawn Albers, Daisy Y. Wong, R. Jane Rylett, and Robert C. Cumming,
J. Biol. Chem., Oct 2012; 287: 37245 - 37258.
[PDK1]
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Discovery of a novel class of AKT pleckstrin homology domain inhibitors
,Daruka Mahadevan, Garth Powis, Eugene A. Mash, Benjamin George, Vijay M. Gokhale, Shuxing Zhang, Kishore Shakalya, Lei Du-Cuny, Margareta Berggren, M. Ahad Ali, Umasish Jana, Nathan Ihle, Sylvestor Moses, Chloe Franklin, Satya Narayan, Nikhil Shirahatti, and Emmanuelle J. Meuillet,
Mol. Cancer Ther., Sep 2008; 7: 2621 - 2632
[PDK1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205127 | PDK1 (Myc-DDK-tagged)-Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC205127L1 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205127L2 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC205127L3 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205127L4 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,888.00 |
|
| RG205127 | PDK1 (tGFP-tagged) - Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein |
CNY 7,088.00 |
