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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Untagged Clone
CAT＃: SC322129
IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)
CNY 5,488.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322129
TGGGTTTCTGCAGAGTCTACTTCAGAAGCGGAGGCACTGGGAGTCCGGTTTGGGATTGCC
AGGCTGTGGTTGTGAGTCTGAGCTTGTGAGCGGCTGTGGCGCCCCAACTCTTCGCCAGCA TATCATCCCGGCAGGCGATAAACTACATTCAGTTGAGTCTGCAAGACTGGGAGGAACTGG GGTGATAAGAAATCTATTCACTGTCAAGGTTTATTGAAGTCAAAATGTCCAAAAAAATCA GTGGCGGTTCTGTGGTAGAGATGCAAGGAGATGAAATGACACGAATCATTTGGGAATTGA TTAAAGAGAAACTCATTTTTCCCTACGTGGAATTGGATCTACATAGCTATGATTTAGGCA TAGAGAATCGTGATGCCACCAACGACCAAGTCACCAAGGATGCTGCAGAAGCTATAAAGA AGCATAATGTTGGCGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGT TCAAGTTGAAACAAATGTGGAAATCACCAAATGGCACCATACGAAATATTCTGGGTGGCA CGGTCTTCAGAGAAGCCATTATCTGCAAAAATATCCCCCGGCTTGTGAGTGGATGGGTAA AACCTATCATCATAGGTCGTCATGCTTATGGGGATCAATACAGAGCAACTGATTTTGTTG TTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAGTGACGGAACCCAAAAGGTGA CATACCTGGTACATAACTTTGAAGAAGGTGGTGGTGTTGCCATGGGGATGTATAATCAAG ATAAGTCAATTGAAGATTTTGCACACAGTTCCTTCCAAATGGCTCTGTCTAAGGGTTGGC CTTTGTATCTGAGCACCAAAAACACTATTCTGAAGAAATATGATGGGCGTTTTAAAGACA TCTTTCAGGAGATATATGACAAGCAGTACAAGTCCCAGTTTGAAGCTCAAAAGATCTGGT ATGAGCATAGGCTCATCGACGACATGGTGGCCCAAGCTATGAAATCAGAGGGAGGCTTCA TCTGGGCCTGTAAAAACTATGATGGTGACGTGCAGTCGGACTCTGTGGCCCAAGGGTATG GCTCTCTCGGCATGATGACCAGCGTGCTGGTTTGTCCAGATGGCAAGACAGTAGAAGCAG AGTCTGCCCACGGGACTGTAACCCGTCACTACCGCATGTACCAGAAAGGACAGGAGACGT CCACCAATCCCATTGCTTCCATTTTTGCCTGGACCAGAGGGTTAGCCCACAGAGCAAAGC TTGATAACAATAAAGAGCTTGCCTTCTTTGCAAATGCTTTGGAAGAAGTCTCTATTGAGA CAATTGAGGCTGGCTTCATGACCAAGGACTTGGCTGCTTGCATTAAAGGTTTACCCAATG TGCAACGTTCTGACTACTTGAATACATTTGAGTTCATGGATAAACTTGGAGAAAACTTGA AGATCAAACTAGCTCAGGCCAAACTTTAAGTTCATACCTGAGCTAAGAAGGATAATTGTC TTTTGGTAACTAGGTCTACAGGTTTACATTTTTCTGTGTTACACTCAAGGATAAAGGCAA AATCAATTTTGTAATTTGTTTAGAAGCCAGAGTTTATCTTTTCTATAAGTTTACAGCCTT TTTCTTATATATACAGTTATTGCCACCTTTGTGAACATGGCAAGGGACTTTTTTACAATT TTTATTTTATTTTCTAGTACCAGCCTAGGAATTCGGTTAGTACTCATTTGTATTCACTGT CACTTTTTCTCATGTTCTAATTATAAATGACCAAAATCAAGATTGCTCAAAAGGGTAAAT GATAGCCACAGTATTGCTCCCTAAAATATGCATAAAGTAGAAATTCACTGCCTTCCCCTC CTGTCCATGACCTTGGGCACAGGGAAGTTCTGGTGTCATAGATATCCCGTTTTGTGAGGT AGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGCAACTCAAATGTGT TGAAGATACTGCAGTCATTTTTGTAAAGACCTTGCTGAATGTTTCCAATAGACTAAATAC TGTTTAGGCCGCAGGAGAGTTTGGAATCCGGAATAAATACTACCTGGAGGTTTGTCCTCT CCATTTTTCTCTTTCTCCTCCTGGCCTGGCCTGAATATTATACTACTCTAAATAGCATAT TTCATCCAAGTGCAATAATGTAAGCTGAATCTTTTTTGGACTTCTGCTGGCCTGTTTTAT TTCTTTTATATAAATGTGATTTCTCAGAAATTGATATTAAACACTATCTTATCTTCTCCT GAACTGTTGATTTTAATTAAAATTAAGTGCTAATTACCAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_005896 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005896.2, NP_005887.2 |
| RefSeq Size | 2339 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 3417 |
| UniProt ID | O75874 |
| Domains | isodh |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 26831078
[IDH1]
|
|
5-hydroxymethylation of the EBV genome regulates the latent to lytic switch
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 26663912
[IDH1]
|
|
Identification and functional characterization of isocitrate dehydrogenase 1 (IDH1) mutations in thyroid cancer
,null,
Biochemical and biophysical research communications
,PubMed ID 20171178
[IDH1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210582 | IDH1 (Myc-DDK-tagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
|
| RC210582L1 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L2 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RC210582L3 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L4 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RG210582 | IDH1 (tGFP-tagged) - Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 7,088.00 |
|
| SC116430 | IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
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