OCLN (NM_002538) Human Untagged Clone

CAT#: SC322559

OCLN (untagged)-Human occludin (OCLN), transcript variant 1



  "NM_002538" in other vectors (7)

CNY 5,840.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-OCLN Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BLCPMG; PPP1R115; PTORCH1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322559 GTCCCCGGCTGAGCGCTGGCGGTCGGTGCGGCGTCAGGTGCGCCCGCCAGGTGAGCGCGC
TCCCTGGCACCGTTGGCCCCCGGAGGGTCGGGCCCAGTTGCGGCGAGCGGATTGGTTTAT
CTTGGAAGCTAAAGGGCATTGCTCATCCTGAAGATCAGCTGACCATTGACAATCAGCCAT
GTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAGGCCTGATGAATTCAAACCGAATCA
TTATGCACCAAGCAATGACATATATGGTGGAGAGATGCATGTTCGACCAATGCTCTCTCA
GCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCTTCACTTCTACAAATGGACCTCTCC
TCCAGGAGTGATTCGGATCCTGTCTATGCTCATTATTGTGATGTGCATTGCCATCTTTGC
CTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTATGGAACTTCCCTTTTAGGAGGTAG
TGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAGCTACGGAAGTGGCTATGGCTATGG
CTATGGTTATGGCTATGGCTACGGAGGCTATACAGACCCAAGAGCAGCAAAGGGCTTCAT
GTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTTGGTGATCTTTGTTACCAGTGTTAT
AAGATCTGAAATGTCCAGAACAAGAAGATACTACTTAAGTGTGATAATAGTGAGTGCTAT
CCTGGGCATCATGGTGTTTATTGCCACAATTGTCTATATAATGGGAGTGAACCCAACTGC
TCAGTCTTCTGGATCTCTATATGGTTCACAAATATATGCCCTCTGCAACCAATTTTATAC
ACCTGCAGCTACTGGACTCTACGTGGATCAGTATTCGTATCACTACTGTGTTGTGGATCC
CCAGGAGGCCATTGCCATTGTACTGGGGTTCATGATTATTGTGGCTTTTGCTTTAATAAT
TTTCTTTGCTGTGAAAACTCGAAGAAAGATGGACAGGTATGACAAGTCCAATATTTTGTG
GGACAAGGAACACATTTATGATGAGCAGCCCCCCAATGTCGAGGAGTGGGTTAAAAATGT
GTCTGCAGGCACACAGGACGTGCCTTCACCCCCATCTGACTATGTGGAAAGAGTTGACAG
TCCCATGGCATACTCTTCCAATGGCAAAGTGAATGACAAGCGGTTTTATCCAGAGTCTTC
CTATAAATCCACGCCGGTTCCTGAAGTGGTTCAGGAGCTTCCATTAACTTCGCCTGTGGA
TGACTTCAGGCAGCCTCGTTACAGCAGCGGTGGTAACTTTGAGACACCTTCAAAAAGAGC
ACCTGCAAAGGGAAGAGCAGGAAGGTCAAAGAGAACAGAGCAAGATCACTATGAGACAGA
CTACACAACTGGCGGCGAGTCCTGTGATGAGCTGGAGGAGGACTGGATCAGGGAATATCC
ACCTATCACTTCAGATCAACAAAGACAACTGTACAAGAGGAATTTTGACACTGGCCTACA
GGAATACAAGAGCTTACAATCAGAACTTGATGAGATCAATAAAGAACTCTCCCGTTTGGA
TAAAGAATTGGATGACTATAGAGAAGAAAGTGAAGAGTACATGGCTGCTGCTGATGAATA
CAATAGACTGAAGCAAGTGAAGGGATCTGCAGATTACAAAAGTAAGAAGAATCATTGCAA
GCAGTTAAAGAGCAAATTGTCACACATCAAGAAGATGGTTGGAGACTATGATAGACAGAA
AACATAGAAGGCTGATGCCAAGTTGTTTGAGAAATTAAGTATCTGACATCTCTGCAATCT
TCTCAGAAGGCAAATGACTTTGGACCATAACCCCGGAAGCCAAACCTCTGTGAGCATCAC
AAAGTTTTGGTTGCTTTAACATCATCAGTATTGAAGCATTTTATAAATCGCTTTTGATAA
TCAACTGGGCTGAACACTCCAATTAAGGATTTTATGCTTTAAACATTGGTTCTTGTATTA
AGAATGAAATACTGTTTGAGGTTTTTAAGCCTTAAAGGAAGGTTCTGGTGTGAACTAAAC
TTTCACACCCCAGACGATGTCTTCATACCTACATGTATTTGTTTGCATAGGTGATCTCAT
TTAATCCTCTCAACCACCTTTCAGATAACTGTTATTTATAATCACTTTTTTCCACATAAG
GAAACTGGGTTCCTGCAATGAAGTCTCTGAAGTGAAACTGCTTGTTTCCTAGCACACACT
TTTGGTTAAGTCTGTTTTATGACTTCATTAATAATAAATTCCCTGGCCTTTCATATTTTA
GCTACTATATATGTGATGATCTACCAGCCTCCCTATTTTTTTTCTGTTATATAAATGGTT
AAAAGAGGTTTTTCTTAAATAATAAAGATCATGTAAAAGTAAAAAAAAAAAAAAAAAAAA
AAAAAA
Restriction Sites Please inquire     
ACCN NM_002538
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002538.2, NP_002529.1
RefSeq Size 2648 bp
RefSeq ORF 1569 bp
Locus ID 100506658
UniProt ID Q16625
Domains MARVEL
Gene Summary This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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