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CAMKIV (CAMK4) (NM_001744) Human Untagged Clone
CAT＃: SC323332
CAMK4 (untagged)-Kinase deficient mutant (K75M) of Human calcium/calmodulin-dependent protein kinase IV (CAMK4)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | caMK; CaMK-GR; CaMK IV; CaMKIV |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323332 sequence for NM_001744 edited (data generated by NextGen Sequencing)
ATGCTCAAAGTCACGGTGCCCTCCTGCTCCGCCTCGTCCTGCTCTTCGGTCACCGCCAGT GCGGCCCCGGGGACCGCGAGCCTCGTCCCGGATTACTGGATCGACGGCTCCAACAGGGAT GCGCTGAGCGATTTCTTCGAGGTGGAGTCGGAGCTGGGACGGGGTGCTACATCCATTGTG TACAGATGCAAACAGAAGGGGACCCAGAAGCCTTATGCTCTCATGGTGTTAAAGAAAACA GTGGACAAAAAAATCGTAAGAACTGAGATAGGAGTTCTTCTTCGCCTCTCACATCCAAAC ATTATAAAACTTAAAGAGATATTTGAAACCCCTACAGAAATCAGTCTGGTCCTAGAACTC GTCACAGGAGGAGAACTGTTTGATAGGATTGTGGAAAAGGGATATTACAGTGAGCGAGAT GCTGCAGATGCCGTTAAACAAATCCTGGAGGCAGTTGCTTATCTACATGAAAATGGGATT GTCCATCGTGATCTCAAACCAGAGAATCTTCTTTATGCAACTCCAGCCCCAGATGCACCA CTCAAAATCGCTGATTTTGGACTCTCTAAAATTGTGGAACATCAAGTGCTCATGAAGACA GTATGTGGAACCCCAGGGTACTGCGCACCTGAAATTCTTAGAGGTTGTGCCTATGGACCT GAGGTGGACATGTGGTCTGTAGGAATAATCACCTACATCTTACTTTGTGGATTTGAACCA TTCTATGATGAAAGAGGCGATCAGTTCATGTTCAGGAGAATTCTGAATTGTGAATATTAC TTTATCTCCCCCTGGTGGGATGAAGTATCTCTAAATGCCAAGGACTTGGTCAGAAAATTA ATTGTTTTGGATCCAAAGAAACGGCTGACTACATTTCAAGCTCTCCAGCATCCGTGGGTC ACAGGTAAAGCAGCCAATTTTGTACACATGGATACCGCTCAAAAGAAGCTCCAAGAATTC AATGCCCGGCGTAAGCTTAAGGCAGCGGTGAAGGCTGTGGTGGCCTCTTCCCGCCTGGGA AGTGCCAGCAGCAGCCATGGCAGCATCCAGGAGAGCCACAAGGCTAGCCGAGACCCTTCT CCAATCCAAGATGGCAACGAGGACATGAAAGCTATTCCAGAAGGAGAGAAAATTCAAGGC GATGGGGCCCAAGCCGCAGTTAAGGGGGCACAGGCTGAGCTGATGAAGGTGCAAGCCTTA GAGAAAGTTAAAGGTGCAGATATAAATGCTGAAGAGGCCCCCAAAATGGTGCCCAAGGCA GTGGAGGATGGGATAAAGGTGGCTGACCTGGAACTAGAGGAGGGCCTAGCAGAGGAGAAG CTGAAGACTGTGGAGGAGGCAGCAGCTCCCAGAGAAGGGCAAGGAAGCTCTGCTGTGGGT TTTGAAGTTCCACAGCAAGATGTGATCCTGCCAGAGTACTAA Clone variation with respect to NM_001744.4 224 a=>t;225 a=>g >OriGene 5' read for mutant NM_001744 unedited
ACGGTACGACGACCAACGAGCGGAGGCGCGCACGGCGAGGGCACACAAGCAGACACGCCCAGCGAACCGC CAGAACCCCGAAACACGACCCACCAAAGGGCGGCCGCGAACCCGGCACGAGGCGGCGGCGGCGGCGGCGG CCACCGGAGCCCCGCCGCGAAGAAGCACAAAGCCACGGTGCCCCCCCGCCCCGCCTCGTCCTGCTCTTCG GTCACCGCCAGCGCGGCCCCGGGGACCGCGAGCCTCGTCCCGGATACTGGATCGACGGCTCCAACAGGGA TGCGCTGAGCGATTTCTTCGAGGTGGAGTCGGAGCTGGGGACGGGGGTGCTACATCCCTTTGTGGTACAG ATGCAAACAGAAGGGGACCCAGAAAGCCTTATGCCTCTCAGGGTGTTAAAGAAAACATTGACCAAAAAAA TCGTAGAACCTGAAATAGGATTTCTTCCTTGGCCTTCTCACATCCAACCTTTATAAAACTTAAGGAGATT TTTGAAACCCCTACAAAATTCATTCTGGTCCTAAACTTGTTCCAAGAAGGAAAACCTGTTGAATGGATTG GGGAAAAAGGAATTTTACGTGAGCGCGGATGCGCTCCAAATGCCCGTTAAACAAACCCGGGAACCATTTT GCTTTCTATATGAGAAAATGGATTTTCCCCTCGATCTCACACACAAGAGACTCTTTTTACAATCTACCGC CCGATATACACATCACAAACGCTGATATTGGCTCTCTAAATGTGGACATCACATGCTCTCGAGACAATTG TGGAAACCCCGGTGCTCGCCACCTGAATCTAAAGTGTGCCTAGGACCGAGTGACTTGTCGTAGAAATACC TCACCTTACTGGATGAGCCTATGAGAAGGCACTGCTAGTCGGATTCGATTGCATCCTGCACTTGGTGAAG TCACTAGCCCGGCCTGCCCACAATGTCGTGACACACC >SC323332 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSTTKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGGCGGCGGCGGC TTCCGGAGTCCCGCTGCGAAGATGCTCAAAGTCACGGTGCCCTCCTGCTCCGCCTCGTCCTGCTCTTCGG TCACCGCCAGTGCGGCCCCGGGGACCGCGAGCCTCGTCCCGGATT |
| Restriction Sites | Please inquire |
| ACCN | NM_001744 |
| Insert Size | 2620 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001744.3, NP_001735.1 |
| RefSeq Size | 2168 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 814 |
| UniProt ID | Q16566 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Long-term potentiation, Neurotrophin signaling pathway |
| Gene Summary | The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206813 | CAMK4 (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase IV (CAMK4) |
CNY 3,656.00 |
|
| RC206813L1 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase IV (CAMK4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206813L2 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase IV (CAMK4), mGFP tagged |
CNY 5,890.00 |
|
| RC206813L3 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase IV (CAMK4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206813L4 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase IV (CAMK4), mGFP tagged |
CNY 5,890.00 |
|
| RG206813 | CAMK4 (tGFP-tagged) - Human calcium/calmodulin-dependent protein kinase IV (CAMK4) |
CNY 5,256.00 |
|
| SC119077 | CAMK4 (untagged)-Human calcium/calmodulin-dependent protein kinase IV (CAMK4) |
CNY 3,656.00 |
