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MASTL (NM_032844) Human Untagged Clone
CAT＃: SC323345
MASTL (untagged)-Kinase deficient mutant (K62M) of Human microtubule associated serine/threonine kinase-like (MASTL)
CNY 9,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GREATWALL; GW; GWL; MAST-L; THC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323345 sequence for NM_032844 edited (data generated by NextGen Sequencing)
ATGGATCCCACCGCGGGAAGCAAGAAGGAGCCTGGAGGAGGCGCGGCGACTGAGGAGGGC GTGAATAGGATCGCAGTGCCAAAACCGCCCTCCATTGAGGAATTCAGCATAGTGAAGCCC ATTAGCCGGGGCGCCTTCGGGAAAGTGTATCTGGGGCAGAAAGGCGGCAAATTGTATGCA GTAATGGTTGTTAAAAAAGCAGACATGATCAACAAAAATATGACTCATCAGGTCCAAGCT GAGAGAGATGCACTGGCACTAAGCAAAAGCCCATTCATTGTCCATTTGTATTATTCACTG CAGTCTGCAAACAATGTCTACTTGGTAATGGAATATCTTATTGGGGGAGATGTCAAGTCT CTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTGTGAAATATATTTCTGAAGTA GCACTGGCTCTAGACTACCTTCACAGACATGGAATCATCCACAGGGACTTGAAACCGGAC AATATGCTTATTTCTAATGAGGGTCATATTAAACTGACGGATTTTGGCCTTTCAAAAGTT ACTTTGAATAGAGATATTAATATGATGGATATCCTTACAACACCATCAATGGCAAAACCT AGACAAGATTATTCAAGAACCCCAGGACAAGTGTTATCGCTTATCAGCTCGTTGGGATTT AACACACCAATTGCAGAAAAAAATCAAGACCCTGCAAACATCCTTTCAGCCTGTCTGTCT GAAACATCACAGCTTTCTCAAGGACTCGTATGCCCTATGTCTGTAGATCAAAAGGACACT ACGCCTTATTCTAGCAAATTACTAAAATCATGTCTTGAAACAGTTGCCTCCAACCCAGGA ATGCCTGTGAAGTGTCTAACTTCTAATTTACTCCAGTCTAGGAAAAGGCTGGCCACATCC AGTGCCAGTAGTCAATCCCACACCTTCATATCCAGTGTGGAATCAGAATGCCACAGCAGT CCCAAATGGGAAAAAGATTGCCAGGAAAGTGATGAAGCATTGGGCCCAACAATGATGAGT TGGAATGCAGTTGAAAAGTTATGCGCAAAATCTGCAAATGCCATTGAGACGAAAGGTTTC AATAAAAAGGATCTGGAGTTAGCTCTTTCTCCCATTCATAACAGCAGTGCCCTTCCCACC ACTGGACGCTCTTGTGTAAACCTTGCTAAAAAATGCTTCTCTGGGGAAGTTTCTTGGGAA GCAGTAGAACTGGATGTAAATAATATAAATATGGACACTGACACAAGTCAGTTAGGTTTC CATCAGTCAAATCAGTGGGCTGTGGATTCTGGTGGGATATCTGAAGAGCACCTTGGGAAA AGAAGTTTAAAAAGAAATTTTGAGTTGGTTGACTCCAGTCCTTGTAAAAAAATTATACAG AATAAAAAAACTTGTGTAGAGTATAAGCATAACGAAATGACAAATTGTTATACAAATCAA AATACAGGCTTAACAGTTGAAGTGCAGGACCTTAAGCTATCAGTGCACAAAAGTCAACAA AATGACTGTGCTAATAAGGAGAACATTGTCAATTCTTTTACTGATAAACAACAAACACCA GAAAAATTACCTATACCAATGATAGCAAAAAACCTTATGTGTGAACTCGATGAAGACTGT GAAAAGAATAGTAAGAGGGACTACTTAAGTTCTAGTTTTCTATGTTCTGATGATGATAGA GCTTCTAAAAATATTTCTATGAACTCTGATTCATCTTTTCCTGGAATTTCTATAATGGAA AGTCCATTAGAAAGTCAGCCCTTAGATTCAGATAGAAGCATTAAAGAATCCTCTTTTGAA GAATCAAATATTGAAGATCCACTTATTGTAACACCAGATTGCCAAGAAAAGACCTCACCA AAAGGTGTCGAGAACCCTGCTGTACAAGAGAGTAACCAAAAAATGTTAGGTCCTCCTTTG GAGGTGCTGAAAACGTTAGCCTCTAAAAGAAATGCTGTTGCTTTTCGAAGTTTTAACAGT CATATTAATGCATCCAATAACTCAGAACCATCCAGAATGAACATGACTTCTTTAGATGCA ATGGATATTTCGTGTGCCTACAGTGGTTCATATCCCATGGCTATAACCCCTACTCAAAAA AGAAGATCCTGTATGCCACATCAGACCCCAAATCAGATCAAGTCGGGAACTCCATACCGA ACTCCGAAGAGTGTGAGAAGAGGGGTGGCCCCCGTTGATGATGGGCGAATTCTAGGAACC CCAGACTACCTTGCACCTGAGCTGTTACTAGGCAGGGCCCATGGTCCTGCGGTAGACTGG TGGGCACTTGGAGTTTGCTTGTTTGAATTTCTAACAGGAATTCCCCCTTTCAATGATGAA ACACCACAACAAGTATTCCAGAATATTCTGAAAAGAGATATCCCTTGGCCAGAAGGTGAA GAAAAGTTATCTGATAATGCTCAAAGTGCAGTAGAAATACTTTTAACCATTGATGATACA AAGAGAGCTGGAATGAAAGAGCTAAAACGTCATCCTCTCTTCAGTGATGTGGACTGGGAA AATCTGCAGCATCAGACTATGCCTTTCATCCCCCAGCCAGATGATGAAACAGATACCTCC TATTTTGAAGCCAGGAATACTGCTCAGCACCTGACCGTATCTGGATTTAGTCTGTAG Clone variation with respect to NM_032844.3 185 a=>t;1782 c=>t;2616 t=>c >OriGene 5' read for mutant NM_032844 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAATGGCTGCTCG CGGAGGGGCAGTGTACGCGGGGCCGCTGTAGGCTGTCCAGCGATGGATCCCACCGCGGGAAGCAAGAAGG AGCCTGGAGGAGGCGCGGCGACTGAGGAGGGCGTGAATAGGATCGCAGTGCCAAAACCGCCCTCCATTGA GGAATTCAGCATAGTGAAGCCCATTAGCCGGGGCGCCTTCGGGAAAGTGTATCTGGGGCAGAAAGGCGGC AAATTGTATGCAGTAATGGTTGTTAAAAAAGCAGACATGATCAACAAAAATATGACTTCATCAGGTCCAA GCTGAGAAAGATGCACTGCACTAAGCAAAGCCCATTCATTGGCCATTTGTATTAATTCACTGCAGTCCTG CAAACAATGTCTACTTGTAATGGAATTTCTTATTGGGGGAGAAGGCAAGTTTCCCCTCCTAATATATGTT TTTTTGATTAAGGAGTGGCTGGGAAATATATTTCTAAGGAACAATGGCTCCAGGCTACTTCCCGACCTGG AATCCCCAAAGGGACCTGAAACCGGGCATATGCTATTTTATTAAGGTCTATAAACTGACGAATTTGGCTC AAAGGTACTTGGTTGGGATATATGTGTAATACCTACACACACTCATGGCAACCTAGCAGATATTCGAACC AGACAGGTACCTTACGCTTTGGATACCCATGCAAATAACTGCACCTAACTGTTGACTAGTTCAGCTATGC AATTGTCAGACTCATTACAAA >SC323345 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ASACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAATGGCTGCTCGCGGAG GGGCAGTGTACGCGGGGCCGCTGTAGGCTGTCCAGCGATGGATCCCACCGCGGGAAGCAAGAAGGAGCCT GGAGGAGGCGCGGCGACTGAGGAGGGCGTGAATAGGATCGCAGTG |
| Restriction Sites | Please inquire |
| ACCN | NM_032844 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032844.1, NP_116233.1 |
| RefSeq Size | 3084 bp |
| RefSeq ORF | 2637 bp |
| Locus ID | 84930 |
| UniProt ID | Q96GX5 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203858 | MASTL (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2 |
CNY 9,656.00 |
|
| RC203858L1 | Lenti ORF clone of Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2, Myc-DDK-tagged |
CNY 12,056.00 |
|
| RC203858L2 | Lenti ORF clone of Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RC203858L3 | Lenti ORF clone of Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC203858L4 | Lenti ORF clone of Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG203858 | MASTL (tGFP-tagged) - Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2 |
CNY 11,256.00 |
|
| SC103147 | MASTL (untagged)-Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2 |
CNY 9,656.00 |
