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PLK1 (NM_005030) Human Untagged Clone
CAT＃: SC323363
PLK1 (untagged)-Kinase deficient mutant (K82M) of Human polo-like kinase 1 (Drosophila) (PLK1)
CNY 6,744.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PLK; STPK13 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005030, the custom clone sequence may differ by one or more nucleotides
ATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACCGGCCGACCCTGGGAAAGCCGGGGTCCCCG GAGTTGCAGCTCCCGGAGCTCCGGCGGCGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCTAGTGGACCC ACGCAGCCGGCGGCGCTATGTGCGGGGCCGCTTTTTGGGCAAGGGCGGCTTTGCCAAGTGCTTCGAGATC TCGGACGCGGACACCAAGGAGGTGTTCGCGGGCAAGATTGTGCCTAAGTCTCTGCTGCTCAAGCCGCACC AGAGGGAGAAGATGTCCATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCACGTCGTAGGATTCCA CGGCTTTTTCGAGGACAACGACTTCGTGTTCGTGGTGTTGGAGCTCTGCCGCCGGAGGTCTCTCCTGGAG CTGCACAAGAGGAGGAAAGCCCTGACTGAGCCTGAGGCCCGATACTACCTACGGCAAATTGTGCTTGGCT GCCAGTACCTGCACCGAAACCGAGTTATTCATCGAGACCTCAAGCTGGGCAACCTTTTCCTGAATGAAGA TCTGGAGGTGAAAATAGGGGATTTTGGACTGGCAACCAAAGTCGAATATGACGGGGAGAGGAAGAAGACC CTGTGTGGGACTCCTAATTACATAGCTCCCGAGGTGCTGAGCAAGAAAGGGCACAGTTTCGAGGTGGATG TGTGGTCCATTGGGTGTATCATGTATACCTTGTTAGTGGGCAAACCACCTTTTGAGACTTCTTGCCTAAA AGAGACCTACCTCCGGATCAAGAAGAATGAATACAGTATTCCCAAGCACATCAACCCCGTGGCCGCCTCC CTCATCCAGAAGATGCTTCAGACAGATCCCACTGCCCGCCCAACCATTAACGAGCTGCTTAATGACGAGT TCTTTACTTCTGGCTATATCCCTGCCCGTCTCCCCATCACCTGCCTGACCATTCCACCAAGGTTTTCGAT TGCTCCCAGCAGCCTGGACCCCAGCAACCGGAAGCCCCTCACAGTCCTCAATAAAGGCTTGGAGAACCCC CTGCCTGAGCGTCCCCGGGAAAAAGAAGAACCAGTGGTTCGAGAGACAGGTGAGGTGGTCGACTGCCACC TCAGTGACATGCTGCAGCAGCTGCACAGTGTCAATGCCTCCAAGCCCTCGGAGCGTGGGCTGGTCAGGCA AGAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTGGGTGGACTATTCGGACAAG TACGGCCTTGGGTATCAGCTCTGTGATAACAGCGTGGGGGTGCTCTTCAATGACTCAACACGCCTCATCC TCTACAATGATGGTGACAGCCTGCAGTACATAGAGCGTGACGGCACTGAGTCCTACCTCACCGTGAGTTC CCATCCCAACTCCTTGATGAAGAAGATCACCCTCCTTAAATATTTCCGCAATTACATGAGCGAGCACTTG CTGAAGGCAGGTGCCAACATCACGCCGCGCGAAGGTGATGAGCTCGCCCGGCTGCCCTACCTACGGACCT GGTTCCGCACCCGCAGCGCCATCATCCTGCACCTCAGCAACGGCAGCGTGCAGATCAACTTCTTCCAGGA TCACACCAAGCTCATCTTGTGCCCACTGATGGCAGCCGTGACCTACATCGACGAGAAGCGGGACTTCCGC ACATACCGCCTGAGTCTCCTGGAGGAGTACGGCTGCTGCAAGGAGCTGGCCAGCCGGCTCCGCTACGCCC GCACTATGGTGGACAAGCTGCTGAGCTCACGCTCGGCCAGCAACCGTCTCAAGGCCTCCTAA >OriGene 5' read for mutant NM_005030 unedited
CCGCCGTTTCAGCAACTGGGCGGTAGGCGTGTACGGTTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGCAGCGCAGC TTCGGGAGCATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACCGGCCGACCCTGGGAAAGCCG GGGTCCCCGGAGTTGCAGCTCCCGGAGCTCCGGCGGCGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCT AGTGGACCCACGCAGCCGGCGGCGCTATGTGCGGGGCCGCTTTTTTGGGCAAGGGCGGCTTTGCCATGTG TGTGTGTGATCTCGGACGCGGACACCAAGGAGGTGTTCGCGGGCATGATTGTGCCTAAGTCTCTGCTGCG CAAGCCGCACCAGAGGGAGGAGGATGTCCATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCACGT CGTAGATTGCACGCTTTTTCGAGACACGACTTCGTGTCGTGTGTGAGCTTCTGCCGCCGAGGTCCCTTCT GAGCTGCCCAGAAGGAGGAAGCCCTGACTGACTGAGCCCGACCTACTACGCAATTGTGCCTGCTGCAGTA CTGCCGGACCGAGTTATTCTCTCGAACTCTAGCCTTGCCAACGTTTCTGAATTGAAACTCGGAGGTGAAA TCGGGAAATTGCTTGCACACAGTGTCAAATTGACGAGAGAGAAACCGTGTGGGCATCCATTCATCTCCAG TGCGTGAACGAAAGGCCAATCAAGAGTTGTGCACTGGTTCATAGTCACGTAAGACCACTTTTGACTCTCT CCATGAACACTCGGTCATAGAACGATTAGGTCTCAGACATTCTGTCGACTCT >SC323363 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCTTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGCAGCGCAGCTTCGGGAGC ATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACCGGCCGACCCTGGGAAAGCCGGGGTCCCCG GAGTTGCAGCTCCCGGAGCTCCGGCGGCGGCTCCACCGGCGAAAG |
| Restriction Sites | Please inquire |
| ACCN | NM_005030 |
| Insert Size | 2300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005030.3, NP_005021.2 |
| RefSeq Size | 2204 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 5347 |
| UniProt ID | P53350 |
| Domains | pkinase, POLO_box, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201795 | PLK1 (Myc-DDK-tagged)-Human polo-like kinase 1 (PLK1) |
CNY 6,736.00 |
|
| RC201795L1 | Lenti ORF clone of Human polo-like kinase 1 (PLK1), Myc-DDK-tagged |
CNY 9,136.00 |
|
| RC201795L2 | Lenti ORF clone of Human polo-like kinase 1 (PLK1), mGFP tagged |
CNY 9,136.00 |
|
| RC201795L3 | Lenti ORF clone of Human polo-like kinase 1 (PLK1), Myc-DDK-tagged |
CNY 9,136.00 |
|
| RC201795L4 | Lenti ORF clone of Human polo-like kinase 1 (PLK1), mGFP tagged |
CNY 9,136.00 |
|
| RG201795 | PLK1 (tGFP-tagged) - Human polo-like kinase 1 (PLK1) |
CNY 8,336.00 |
|
| SC110978 | PLK1 (untagged)-Human polo-like kinase 1 (PLK1) |
CNY 6,744.00 |
