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PFTK1 (CDK14) (NM_012395) Human Untagged Clone
CAT＃: SC323368
CDK14 (untagged)-Kinase deficient mutant (K164M) of Human PFTAIRE protein kinase 1 (PFTK1)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PFTAIRE1; PFTK1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_012395, the custom clone sequence may differ by one or more nucleotides
ATGCACGGTTACTTTGGCTGCAATGCTGCTGCAGAGCCCGGTTACTCTGCCTTCGTGGGAACTCCACAGA TATGTGTCACAAAGATGTCTACACGGAACTGCCAGGGAATGGACTCAGTGATCAAACCCCTGGACACAAT TCCTGAGGATAAAAAAGTCAGAGTTCAGAGGACACAGAGCACTTTTGACCCATTTGAGAAACCAGCTAAT CAAGTAAAGAGGGTGCATTCTGAGAACAATGCTTGCATTAACTTTAAGACCTCCTCCACTGGCAAAGAGT CACCTAAAGTTAGGCGGCACTCCAGCCCCAGCTCGCCAACAAGTCCCAAATTTGGAAAAGCTGACTCATA TGAAAAGCTGGAAAAACTAGGGGAAGGATCTTATGCTACAGTATACAAAGGGAAAAGCAAGGTAAATGGG AAGTTGGTAGCTCTGAAGGTGATCAGGCTGCAGGAAGAAGAAGGGACACCTTTCACAGCTATCAGGGAAG CTTCTCTTTTAAAAGGACTAAAACATGCTAACATAGTGCTACTTCATGACATCATCCATACCAAGGAGAC GCTGACACTTGTGTTTGAATATGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGGGGGCTG CATCCAGATAATGTGAAGTTGTTTTTATTTCAGTTGCTGCGAGGTCTGTCTTACATCCACCAGCGTTATA TTTTGCACAGAGACCTGAAACCACAGAACCTTCTGATCAGTGACACGGGGGAGTTAAAGCTGGCAGATTT CGGTCTTGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAACGAAGTGGTTACCTTGTGGTACAGA CCTCCAGATGTCCTTCTAGGCTCAACAGAATATTCCACCTGCCTTGACATGTGGGGAGTAGGTTGCATCT TTGTTGAAATGATCCAAGGAGTTGCTGCTTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGAAT ATTTCTGGTTCTTGGAACACCAAATGAGGACACATGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCA GAACGCTTTACCCTGTACAGCTCTAAAAACCTTAGACAAGCATGGAATAAGCTCAGCTATGTGAACCATG CAGAGGACCTGGCCTCCAAGCTCCTACAATGTTCCCCAAAGAACAGACTGTCGGCACAGGCTGCCTTGAG CCACGAGTATTTTAGTGACCTGCCGCCACGGCTATGGGAACTCACCGACATGTCTTCTATTTTTACTGTC CCAAATGTGAGATTGCAACCAGAAGCTGGAGAAAGCATGCGGGCCTTTGGGAAAAACAATAGTTATGGCA AAAGTCTATCAAACAGCAAGCACTGA >OriGene 5' read for mutant NM_012395 unedited
CCCGCCCGTCTCAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGTTGTAG TACGGCCGTACCATTTCAGCTTGCTAGTGCAGAAAGATGTGAATTCAGTTGCTGTATGAGCCTGGCCTGG TGCAAACACATTGCTAGAGACATGTTAAAGAGTTCCAGGTGAATCAAGCCTGAGGGAGACAACAGCAAAA CGATATGTGTCACAAAGATGTCTACACGGAACTGCCAGGGGAATGGACTCAGTGATCAAACCCCTGGACA CAATTCCTGAGGATTAAAAAAAGTCAGAGTTTCAGAGGACACAGAGCCACTTTTTGACCCATTGAAGAAA CCCAGCTTAATCAAGTTAAAGAGGGTGGCATTCGAAAAACAAGGCTTGGCATTACCTTTAGAACCTCCCC AACTGCCAAGAAGTCACCTAAGGTTAGGCGGACTTCAGCCCCAGCTCGGCCACAGGGTCCCAATTGGAAA AGCGCTGACTATATAGAAAAAGCTGGGAAAACTTAGGGGGAGGGATTATGCCCACGTATACAAAAGGAAA AAAACAGTGAATGGGAGATTTTGTAGTCTGAGGTGATATCGCGCTGCGAGAAGAAAGGACACTTTTACAC ACTATCGGGGACTCTCTTTAAAGAGCAACACGCTAACATGGCATCTTTGAACTCTCTACCAGGGACGTGA CCTTGTGTGGAATGTGCCACCTGATATTGCTACCTGACAACCCTGGGCCGACTCAGAATTGGAGTGCTAT CATTGCGCAAGCTGCCTATACCACGCTATATGCGAACTGACCAGGCTGTCATAGCAGGGGATAAGCGCG >SC323368 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ACKATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGTTGTAGTACGGCCG TACCATTTCAGCTTGCTAGTGCAGAAAGATGTGAATTCAGTTGCTGTATGAGCCTGGCCTGGTGCAAACA CATTGCTAGAGACATGTTAAAGAGTTCCAGGTGAATCAAGCCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_012395 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012395.2, NP_036527.1 |
| RefSeq Size | 4953 bp |
| RefSeq ORF | 1356 bp |
| Locus ID | 5218 |
| UniProt ID | O94921 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224426 | CDK14 (Myc-DDK-tagged)-Human cyclin-dependent kinase 14 (CDK14) |
CNY 3,656.00 |
|
| RC224426L1 | Lenti ORF clone of Human cyclin-dependent kinase 14 (CDK14), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC224426L2 | Lenti ORF clone of Human cyclin-dependent kinase 14 (CDK14), mGFP tagged |
CNY 5,890.00 |
|
| RC224426L3 | Lenti ORF clone of Human cyclin-dependent kinase 14 (CDK14), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224426L4 | Lenti ORF clone of Human cyclin-dependent kinase 14 (CDK14), mGFP tagged |
CNY 5,890.00 |
|
| RG224426 | CDK14 (tGFP-tagged) - Human cyclin-dependent kinase 14 (CDK14) |
CNY 4,370.00 |
|
| SC310370 | CDK14 (untagged)-Human cyclin-dependent kinase 14 (CDK14) |
CNY 7,220.00 |
