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SRPK1 (NM_003137) Human Untagged Clone
CAT＃: SC323371
SRPK1 (untagged)-Kinase deficient mutant (K109M) of Human SFRS protein kinase 1 (SRPK1)
CNY 4,888.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SFRSK1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323371 sequence for NM_003137 edited (data generated by NextGen Sequencing)
ATGGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGAC AAAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGAT CTACCAGAGCAGGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAAT GATTATTGTAAAGGAGGTTATCATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATAC CATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCATGGGATATT CAGGGGAAGAAATTTGTGGCAATGAAAGTAGTTAAAAGTGCTGAACATTACACTGAAACA GCACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAAT AGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACAT ATCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAAGTGGATCATCAAATCCAAT TATCAGGGGCTTCCACTGCCTTGTGTCAAAAAAATTATTCAGCAAGTGTTACAGGGTCTT GATTATTTACATACCAAGTGCCGTATCATCCACACTGACATTAAACCAGAGAACATCTTA TTGTCAGTGAATGAGCAGTACATTCGGAGGCTGGCTGCAGAAGCAACAGAATGGCAGCGA TCTGGAGCTCCTCCGCCTTCCGGATCTGCAGTCAGTACTGCTCCCCAGCCTAAACCAGCT GACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAA TTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAA AAAAGACCAAACAAGCAAGAAGAATCAGAGAGTCCTGTTGAAAGACCCTTGAAAGAGAAC CCACCTAATAAAATGACCCAAGAAAAACTTGAAGAGTCAAGTACCATTGGCCAGGATCAA ACGCTTATGGAACGTGATACAGAGGGTGGTGCAGCAGAAATTAATTGCAATGGAGTGATT GAAGTCATTAATTATACTCAGAACAGTAATAATGAAACATTGAGACATAAAGAGGATCTA CATAATGCTAATGACTGTGATGTCCAAAATTTGAATCAGGAATCTAGTTTCCTAAGCTCC CAAAATGGAGACAGCAGCACATCTCAAGAAACAGACTCTTGTACACCTATAACATCTGAG GTGTCAGACACCATGGTGTGCCAGTCTTCCTCAACTGTAGGTCAGTCATTCAGTGAACAA CACATTAGCCAACTTCAAGAAAGCATTCGGGCAGAGATACCCTGTGAAGATGAACAAGAG CAAGAACATAACGGACCACTGGACAACAAAGGAAAATCCACGGCTGGAAATTTTCTTGTT AATCCCCTTGAGCCAAAAAATGCAGAAAAGCTCAAGGTGAAGATTGCTGACCTTGGAAAT GCTTGTTGGGTGCACAAACATTTCACTGAAGATATTCAAACAAGGCAATATCGTTCCTTG GAAGTTCTAATCGGATCTGGCTATAATACCCCTGCTGACATTTGGAGCACGGCATGCATG GCCTTTGAACTGGCCACAGGTGACTATTTGTTTGAACCTCATTCAGGGGAAGAGTACACT CGAGATGAAGATCACATTGCATTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTC ATTGTGGCAGGAAAATATTCCAAGGAATTTTTCACCAAAAAAGGTGACCTGAAACATATC ACGAAGCTGAAACCTTGGGGCCTTTTTGAGGTTCTAGTGGAGAAGTATGAGTGGTCGCAG GAAGAGGCAGCTGGCTTCACAGATTTCTTACTGCCCATGTTGGAGCTGATCCCTGAGAAG AGAGCCACTGCCGCCGAGTGTCTCCGGCACCCTTGGCTTAACTCCTAA Clone variation with respect to NM_003137.4 >OriGene 5' read for mutant NM_003137 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTGGCGATTACC GGTCTCACCATGGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGACA AAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGATCTACCAGAGCA GGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAATGATTATTGTAAAGGATGTTAT CATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATACCATGTGATCCGAAAGTTAGGCTGGGGACACT TTTCAACAGTATGGTTATCATGGGATATTCAGGGGAAGAAATTTGTGGCAATGATGGTAGTTAAAAGTGC TGAACATTACACTGAAACAGCACTAGATGAATCCCGGTTGCTGAAGTCAGTTCGCAATTTCAGACCCTAT GATCCAAATAGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACATA TCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAGGTGGATCATCAAATCCAATTATTCAGGGGC TTCCACCTGCCTTGTTGTCAAAAAATTATTCAGCAAGTGTGTTACAGGTTCTTGAATTATTTACATACAA GTGGCCGTTATCATTCCACAACTGGACTTAACCAGAGAACTTCTTTATTTGTCATGGATGGACAGTCTTC GAGGCGTGCGCTCCAAGAGACACACGAAATGGCACCGATTCGGGACTCTCTCGGCTTCGGATTCGCAGAT CCAGTATCGCGTTTCCAGCTAACAGTTTGCGAAAATGTGTCCGAGAATAGAGA >SC323371 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GGACTATTCATGGGAGWACCATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCA TGGGATATTCAGGGGAAGAAATTTGTGGCAATGATGGTAGTTAAAAGTGCTGAACATTACACTGAAACAG CACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAATAGAGAAATGGT TGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAAC |
| Restriction Sites | Please inquire |
| ACCN | NM_003137 |
| Insert Size | 4480 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003137.3, NP_003128.3 |
| RefSeq Size | 4361 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 6732 |
| UniProt ID | Q96SB4 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) represents the shorter transcript. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205315 | SRPK1 (Myc-DDK-tagged)-Human SRSF protein kinase 1 (SRPK1), transcript variant 1 |
CNY 4,880.00 |
|
| RC205315L1 | Lenti ORF clone of Human SRSF protein kinase 1 (SRPK1), transcript variant 1, Myc-DDK-tagged |
CNY 7,280.00 |
|
| RC205315L2 | Lenti ORF clone of Human SRSF protein kinase 1 (SRPK1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC205315L3 | Lenti ORF clone of Human SRSF protein kinase 1 (SRPK1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205315L4 | Lenti ORF clone of Human SRSF protein kinase 1 (SRPK1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG205315 | SRPK1 (tGFP-tagged) - Human SRSF protein kinase 1 (SRPK1), transcript variant 1 |
CNY 6,480.00 |
|
| SC118183 | SRPK1 (untagged)-Human SRSF protein kinase 1 (SRPK1), transcript variant 1 |
CNY 4,888.00 |
