STK23 (SRPK3) (NM_014370) Human Untagged Clone

CAT#: SC323379

SRPK3 (untagged)-Kinase deficient mutant (K108M) of Human SFRS protein kinase 3 (SRPK3)



  "NM_014370" in other vectors (7)

CNY 4,232.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
SRPK3 mouse monoclonal antibody,clone OTI6F5
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MSSK-1; MSSK1; STK23
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_014370, the custom clone sequence may differ by one or more nucleotides


ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGCTCACAGGCCT
CCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTGCCTCAGATGCTGCAGGGCCT
TCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGACTACTGCAAGGGCGGCTACCACCCTGTGAAG
ATCGGCGACGTGTTCAATGGGCGGTACCACGTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCT
GGCTCTGCTGGGACATCCAGCGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACAC
GGAGACAGCTGTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA
GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTGTGCATGGTGC
TGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTACCAGGGCCTGCCCGTGCCCTG
CGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGACTACCTCCACACCAAGTGCAAGATCATCCAC
ACGGACATCAAGCCCGAGAACATCTTGCTGTGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGG
CCACGGAGTGGCAACAGGCAGGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGT
CTTGCAGACCGGTAAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGG
CTGCTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCTGAGGACT
CTGGCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCACCCCGGGGGCGCCAGAGC
AGGTCCCTCCCCAGCCTCTTCCTCCCCCGCCCCAGGGGGCGGCCGTAGCCTCAGCGCGGGCTCACAGACC
TCAGGCTTCTCCGGCTCCCTCTTCTCTCCTGCCTCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAG
AGACCGGGGGCCTCCTGTCGCCTAGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCC
CCAAAATGCAGATAAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTC
ACGGAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGCCCCCCGG
CAGACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTACCTGTTCGAGCCGCATTC
TGGAGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATAGTGGAGCTTCTGGGGGACATCCCCCCA
GCCTTCGCCCTCTCAGGCCGCTATTCCCGGGAGTTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACA
ATCTCAAGCACTGGGGCCTGTACGAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACA
GTTCAGCGCCTTTCTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTC
CAGCACCCCTGGCTCAACCCCTAG


>OriGene 5' read for mutant NM_014370 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCAC
CGGAGCTGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGC
GGCAGTAGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACAC
CGGTGCCTCAGATGCTGCAGGGGCCTTTCTGGGCTCCGACGACGAGGGAACAGGGAAAGACCCCAAAGAA
CTACTGCAAGGGCGGGCTACCCACCCCTGTTGAAAGAATCGGGCGACGTGGTTCATGGGGGCGGGTCCCA
CGTGGGTGGGGGCAAAACTGGGCCTGGGGCCATTTTCTCCACCCGTCCTGGCTTGGCCTGGGACATCCCA
CCGCCAGGCCCCTTTGGGGCCCCTCATTTTGTTAAAAAATTCCGGGCCATTTACACGGAAAAGGTTTGGG
GAAAAAAATTAACCCCCCGAAAATTGGTCCCGGAAACCCGAACCCCCTGGGCCCCAAAAAAAAAAAAACA
TTTGGCCCATCTATTTGGGAAATTTTCGGGTTTCCCGGGTTTTAAGGGGGCCCCGGGGGGCCCAGGGCCC
CGAAAATCTCCTCCCCCCCCCCCCCCTAAAGGGGTATTATAGTTGCCACTTCAACAGGGCCCTTCCCTTC
CCCCGGGTAGAAAAATACTCAGGGGGCGAGGGTGCTCGACGCTCTGGAACTCCCCCACCCCATTTGTGCG
ATATTTTCTCCCGCGGAGGTATTCGCCACGAGAAACTTGTTGAGGGTGGGGGGGATCATAATATAGGGAC
GCGTGATAGTGGCGACCGCAGATGATACAGAGAGGCGGGAGCCCCTCTCACATGATGCTAGACTCATTCT
CTGCAAGGGAGATTG
>SC323379 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCACCGGAGC
TGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGT
AGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCC
Restriction Sites Please inquire     
ACCN NM_014370
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014370.2, NP_055185.2
RefSeq Size 2040 bp
RefSeq ORF 1704 bp
Locus ID 26576
UniProt ID Q9UPE1
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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