RPS6KA3 (NM_004586) Human Untagged Clone

CAT#: SC323412

RPS6KA3 (untagged)-Kinase deficient mutant (K100M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)



  "NM_004586" in other vectors (9)

CNY 5,432.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal RSK2 (RPS6KA3) Antibody (N-term)
    • 400 ul

CNY 6,314.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19; p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004586, the custom clone sequence may differ by one or more nucleotides


ATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGACAGCGCTGAGA
ATGGACAGCAAATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTAT
CAAAGAAATTGCAATCACACATCATGTAAAGGAAGGACATGAAAAGGCAGATCCTTCCCAGTTTGAACTT
TTAAAAGTATTAGGGCAGGGATCATTTGGAAAGGTTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTA
GGCAGCTTTATGCCATGAAGGTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCGGACAAAAAT
GGAACGTGATATCTTGGTAGAGGTTAATCATCCTTTTATTGTCAAGTTGCATTATGCTTTTCAAACTGAA
GGGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGAGGAGATTTGTTTACACGCTTATCCAAAGAGGTGA
TGTTCACAGAAGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCTTTAGACCATCTACATAGCCT
GGGAATAATTTATAGAGACTTAAAACCAGAAAATATACTTCTTGATGAAGAAGGTCACATCAAGTTAACA
GATTTCGGCCTAAGTAAAGAGTCTATTGACCATGAAAAGAAGGCATATTCTTTTTGTGGAACTGTGGAGT
ATATGGCTCCAGAAGTAGTTAATCGTCGAGGTCATACTCAGAGTGCTGACTGGTGGTCTTTTGGTGTGTT
AATGTTTGAAATGCTTACTGGTACACTCCCTTTCCAAGGAAAAGATCGAAAAGAAACAATGACTATGATT
CTTAAAGCCAAACTTGGAATGCCACAGTTTTTGAGTCCTGAAGCGCAGAGTCTTTTACGAATGCTTTTCA
AGCGAAATCCTGCAAACAGATTAGGTGCAGGACCAGATGGAGTTGAAGAAATTAAAAGACATTCATTTTT
CTCAACGATAGACTGGAATAAACTGTATAGAAGAGAAATTCATCCGCCATTTAAACCTGCAACGGGCAGG
CCTGAAGATACATTCTATTTTGATCCTGAGTTTACTGCAAAAACTCCCAAAGATTCACCTGGCATTCCAC
CTAGTGCTAATGCACATCAGCTTTTTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCA
AGCTATGCAGACAGTTGGTGTACATTCAATTGTTCAGCAGTTACACAGGAACAGTATTCAGTTTACTGAT
GGATATGAAGTAAAAGAAGATATTGGAGTTGGCTCCTACTCTGTTTGCAAGAGATGTATACATAAAGCTA
CAAACATGGAGTTTGCAGTGAAGATTATTGATAAAAGCAAGAGAGACCCAACAGAAGAAATTGAAATTCT
TCTTCGTTATGGACAGCATCCAAACATTATCACTCTAAAGGATGTATATGATGATGGAAAGTATGTGTAT
GTAGTAACAGAACTTATGAAAGGAGGTGAATTGCTGGATAAAATTCTTAGACAAAAATTTTTCTCTGAAC
GAGAGGCCAGTGCTGTCCTGTTCACTATAACTAAAACCGTTGAATATCTTCACGCACAAGGGGTGGTTCA
TAGAGACTTGAAACCTAGCAACATTCTTTATGTGGATGAATCTGGTAATCCGGAATCTATTCGAATTTGT
GATTTTGGCTTTGCAAAACAGCTGAGAGCGGAAAATGGTCTTCTCATGACTCCTTGTTACACTGCAAATT
TTGTTGCACCAGAGGTTTTAAAAAGACAAGGCTATGATGCTGCTTGTGATATATGGAGTCTTGGTGTCCT
ACTCTATACAATGCTTACCGGTTACACTCCATTTGCAAATGGTCCTGATGATACACCAGAGGAAATATTG
GCACGAATAGGTAGCGGAAAATTCTCACTCAGTGGTGGTTACTGGAATTCTGTTTCAGACACAGCAAAGG
ACCTGGTGTCAAAGATGCTTCATGTAGACCCTCATCAGAGACTGACTGCTGCTCTTGTGCTCAGACATCC
TTGGATCGTCCACTGGGACCAACTGCCACAATACCAACTAAACAGACAGGATGCACCACATCTAGTAAAG
GGTGCCATGGCAGCTACATATTCTGCTTTGAACCGTAATCAGTCACCAGTTTTGGAACCAGTAGGCCGCT
CTACTCTTGCTCAGCGGAGAGGTATTAAAAAAATCACCTCAACAGCCCTGTGA


>OriGene 5' read for mutant NM_004586 unedited
ACGCCGTTGTAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGATGCCGCTGG
CGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGACAGCGCTGAGAATGGACAGCA
AATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTATCAAAGAAATT
GCAATCACACATCATGTAAAGGAAGGACATGAAAAAGGCAGATCCCTTCCCCAGTTTGAACTTTTTAAAA
GTATTAGGGCAGGGGATCATTTTGGAAAGGTTTTCTTAGTAAAAAAAATCTCAGGCTCTGATGCCTAGGC
AGCTTTTATGCCCATGATGGTATTTGGAGAAAGGCCACCCTGAAAAGTCCAAAACCGATTTCGGACAAAA
ATTGGAACCGTGATATTCTGGGTTGAAGGTTAATCATCCCTTTTAATGTGCAAGGTTGACATTAGCGCTT
TCAAAACTGGAGGGGGAGTTTGATATCTAATTTGGGTTTTTTCTCAGGGGGAGGGGAGATTTGTTTACAC
GCTATATCCAAGAGAGTGAGATGTACAGAAAGAGAAGTGTCAAATTTCTATTGGCTGAGACTTTGCATGT
GCTTTGACACCTCTCACATCCCGGGATATATTTATAGACACTACCCGAATATACTCTTTGTGAGGAAGGG
CACTCGTTTCACATATCTGCGCCATATAAGTCTCTGTCACCGAGAAAGAGGCATCTCTTTGGACACTGGT
ATAGTCCCAGATTATACTCAGGCGTCCCCACAGGTCCTGCGGGCCTTGTGGTTTAGATGCAGACTCTGGA
CAACGTTCAGGATCACGGAACGGCTGTAATCTCAGGGCACATGTGATGCACCAGTTGTTACTCGTACACG
A
>SC323412 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ASACTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGATGCCGCTGGCGCA
GCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGACAGCGCTGAGAATGGACAGCAAATT
ATGGATGAACCTATGGGAGAGGAGGAGATTAACCCACAAACTGAA
Restriction Sites Please inquire     
ACCN NM_004586
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004586.2, NP_004577.1
RefSeq Size 7723 bp
RefSeq ORF 2223 bp
Locus ID 6197
UniProt ID P51812
Domains pkinase, S_TK_X, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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