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CDKL5 (NM_003159) Human Untagged Clone
CAT＃: SC323424
CDKL5 (untagged)-Kinase deficient mutant (K42M) of Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I
CNY 7,176.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CFAP247; DEE2; EIEE2; ISSX; STK9 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003159, the custom clone sequence may differ by one or more nucleotides
ATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGATCCTTGGGGTTGTAGGTGAAGGAGCCT ATGGAGTTGTACTTAAATGCAGACACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAG TGAAGAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCGGACTCTCAAGCAGGAA AACATTGTGGAGTTGAAGGAAGCATTTCGTCGGAGGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAA AAAATATGCTCGAATTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTACATCTA TCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTGTCCATCGAGATATAAAACCAGAAAAT CTCTTAATCAGCCACAATGATGTCCTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCA ATAATGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAACTCTTACTTGGCGCTCC CTATGGAAAGTCCGTGGACATGTGGTCGGTGGGCTGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTA TTTCCTGGAGAAAGTGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCATCTGAGC AGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGGTTTCCAGCTGTTAACCATCCTCAGTC CTTGGAAAGAAGATACCTTGGAATTTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTG GACCCAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAAACCCAGAGACTTCTGG ATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACCTTACCATGTGGAAAGCAGCACATTGTCTAATAGAAA CCAAGCCGGCAAAAGTACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAACCTGAGT GTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAGCTTCCTAAATGGAAACCTTGCTGGAG CTAGTCTTAGTCCACTGCACACCAAAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCT CACCAACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAACAGAGTTTGATTTTAAT ATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAAAGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACC GCCACTCATTCATGGAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAGTCGGCA TAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCTACAGGACCAAGGCCAAAAGCCATGGG GCACTGAGTGACTCCAAGTCTGTGAGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCA GTAGGTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCCCCACCAGACACAGTGA CACGAGAACTTTGCTCAGCCCTTCTGGAAGAAATAACCGAAATGAGGGAACGCTGGACTCACGTCGAACC ACAACCAGACATTCTAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATTCCCATT CACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTACACCAGCCCCTTTTCTTCCCAGCAACG TCCTCATAGGCATTCTATGTATGTGACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGC ATAGGGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAGCCTGGAGAACAGCTCC CTCCAGAGATGACTGTGGCAAGATCTTCGGTCAAAGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATAC ACGCCAGAAGTCTGAGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAAGAAAAT AGACACCTATACAATGATCCTGTGCCAAGGAGAGTTGGTAGCTTTTACAGAGTGCCATCTCCACGTCCAG ACAATTCTTTCCATGAAAATAATGTGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAAC CAACCACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATATTAGTCATTCAGAGCAA CTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGTCAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTAC CCAATTCCGACAGCCCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAGCAGCAG ACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCCAAAGCCAGCCATTAAAATCACTGCGC AAGTTGTTACATCTCTCTTCGGCCTCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAG CTCAACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGGCCTCTGGCGGGAGCAG CAACATCCGGCAGGAACCCGCACCGAAGGGCAGGCCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGC AGAAGACAGAGACACCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAACAAGGAG AATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGCGTCCCAAACCGAGCCCTTCATCGTCC AATCTCCAGTCCTGCTCCCTATCCAGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTC CGAGGCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTCGTGAGACACGTTATGA GGGAAGCCCTGATTCACAGGGCCCAGGTAAACCAAGCTGCGCTCCTGACATACCATGAGAATGCGGCACT GACGGGCAAGTGA >OriGene 5' read for mutant NM_003159 unedited
CCCGCCCGTATCAGCAATTGGGCGGTAGGCGCTGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTA GTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGACGG CGTCCTCAGGAGCTGTGGGGTCCCCTGCTAGAAGTGGGGGACTCGGCGGGGGAGTCATTTAATACTTCAT GATTAGAACAAATATGTGAAAGTTCCCACCAACCAGTGAGAATTTCTTCCTTCAGACGGTTTTGGATCTT ACTGCACAGCTTTCTGAGAAGTTCTTTTGGTGCCATGTTTTGTGGGCTTGCATCAAAAGAAGGAGTTTGT CTTTCATGAAGAATTCCTAAACAATTGGTAAATGTGGATTGAAATAAAATTTGAGAATCCTTGGGGGTGT GTAGGTGGAAGAGACCCCTATGGGATTTGGTACTTAATGGGCAAACCCCAAGGGAACCAACATGGAAATT GGGGGCAAATCATGAAAATTCAGGGCCGTGTGAAAAAAAATGAAAAAGTTCAAAAAAACAAACTTTCCAA AGACCTAAAATGGCTTCGGAACTCTAACCCGGAAAAACCTTTGGGGGATTAAAAGGAACCCTTTTCCCCG AGGGGAAAATTTTCCCTTGGGGTTTGAGAATTTTTAAAAAAAAATTCCCCAAATTTGGGGGAAAAATGCC CAAGGGGTTTCCCCCCGGAAAAATTAAACGCCACTTTTTTCCTATATCGGGCGTTTTCTGGTGGCGGTAA AAAAATATTTTCCCCCACAAAAATAAACCCAAAAATCCTATAACCCCCCAGAAGGGCCCAAACTGTGAGT TTTGGTTTTTCGGATATCTCGAAAGACGCATAGCCGTTTTTACCACACGTGTCCCCCACATGGGGTGTTG CCCCCAAACATCTTATGGCGCCTCTCAGAAAAACCCTGCAGACTGAGTCGGAGCCGCGTTCTGGGGCGTA CACGCGCGAACATTTCTTCTGAGCAGAGAAGTAGACCCTCTCGTTATATAGAG >SC323424 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTCKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGACGGCGTCCTCAGGAG CTGTGGGGTCCCCTGCTAGAAGTGGGGGACTCGGCGGGGGAGTCATTTAATACTTCATGATTAGAACAAA TATGTGAAAGTTCCCACCAACCAGTGAGAATTTCTTCCTTCAGAC |
| Restriction Sites | Please inquire |
| ACCN | NM_003159 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003159.1, NP_003150.1 |
| RefSeq Size | 3434 bp |
| RefSeq ORF | 3093 bp |
| Locus ID | 6792 |
| UniProt ID | O76039 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (I) represents the longer transcript. Variants I and II encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220764 | CDKL5 (Myc-DDK-tagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 7,176.00 |
|
| RC220764L1 | Lenti-ORF clone of CDKL5 (Myc-DDK-tagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 9,576.00 |
|
| RC220764L2 | Lenti-ORF clone of CDKL5 (mGFP-tagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 10,260.00 |
|
| RC220764L3 | Lenti-ORF clone of CDKL5 (Myc-DDK-tagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 10,260.00 |
|
| RC220764L4 | Lenti-ORF clone of CDKL5 (mGFP-tagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 10,260.00 |
|
| RG220764 | CDKL5 (tGFP-tagged) - Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 9,220.00 |
|
| SC310000 | CDKL5 (untagged)-Human cyclin-dependent kinase-like 5 (CDKL5), transcript variant I |
CNY 7,176.00 |
