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PCTAIRE3 (CDK18) (NM_002596) Human Untagged Clone
CAT＃: SC323457
CDK18 (untagged)-Kinase deficient mutant (K203M) of Human PCTAIRE protein kinase 3 (PCTK3), transcript variant 3
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PCTAIRE; PCTAIRE3; PCTK3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323457 sequence for NM_002596 edited (data generated by NextGen Sequencing)
ATGATCATGAACAAGATGAAGAACTTTAAGCGCCGTTTCTCCCTGTCAGTGCCCCGCACT GAGACCATTGAAGAATCCTTGGCTGAATTCACGGAGCAATTCAACCAGCTCCACAACCGG CGGAATGAGAACTTGCAGCTCGGTCCTCTTGGCAGAGACCCCCCGCAGGAGTGCAGCACC TTCTCCCCAACAGACAGCGGGGAGGAGCCGGGGCAGCTCTCCCCTGGCGTGCAGTTCCAG CGGCGGCAGAACCAGCGCCGCTTCTCCATGGAGGACGTCAGCAAGAGGCTCTCTCTGCCC ATGGATATCCGCCTGCCCCAGGAATTCCTACAGAAGCTACAGATGGAGAGCCCAGATCTG CCCAAGCCGCTCAGCCGCATGTCCCGCCGGGCCTCCCTGTCAGACATTGGCTTTGGGAAA CTGGAAACATACGTGAAACTGGACAAACTGGGAGAGGGCACCTATGCCACAGTCTTCAAA GGGCGCAGCAAACTGACGGAGAACCTTGTGGCCCTGATGGAGATCCGGCTGGAGCACGAG GAGGGAGCGCCCTGCACTGCCATCCGAGAGGTGTCTCTGCTGAAGAACCTGAAGCACGCC AATATTGTGACCCTGCATGACCTCATCCACACAGATCGGTCCCTCACCCTGGTGTTTGAG TACCTGGACAGTGACCTGAAGCAGTATCTGGACCACTGTGGGAACCTCATGAGCATGCAC AACGTCAAGATTTTCATGTTCCAGCTGCTCCGGGGCCTCGCCTACTGTCACCACCGCAAG ATCCTGCACCGGGACCTGAAGCCCCAGAACCTGCTCATCAACGAGAGGGGGGAGCTGAAG CTGGCCGACTTTGGACTGGCCAGGGCCAAGTCAGTGCCCACAAAGACTTACTCCAATGAG GTGGTGACCCTGTGGTACAGGCCCCCCGATGTGCTGCTGGGATCCACAGAGTACTCCACC CCCATTGATATGTGGGGCGTGGGCTGCATCCACTACGAGATGGCCACAGGGAGGCCCCTC TTCCCGGGCTCCACAGTCAAGGAGGAGCTGCACCTCATCTTTCGCCTCCTCGGGACCCCC ACAGAAGAGACGTGGCCCGGCGTGACCGCCTTCTCTGAGTTCCGCACCTACAGCTTCCCC TGCTACCTCCCGCAGCCGCTCATCAACCACGCGCCCAGGTTGGATACGGATGGCATCCAC CTCCTGAGCAGCCTGCTCCTGTATGAATCCAAGAGTCGCATGTCAGCAGAGGCTGCCCTG AGTCACTCCTACTTCCGGTCTCTGGGAGAGCGTGTGCACCAGCTTGAAGACACTGCCTCC ATCTTCTCCCTGAAGGAGATCCAGCTCCAGAAGGACCCAGGCTACCGAGGCTTGGCCTTC CAGCAGCCAGGACGAGGGAAGAACAGGCGGCAGAGCATCTTCTGA Clone variation with respect to NM_002596.3 518 a=>t;519 a=>g >OriGene 5' read for mutant NM_002596 unedited
ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGAC ACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGGCACGAGGGCGGCCCCCAGGACA CGCGCTGTGAGTCCCGCGGGCGGTGCGCCTGGGAGGAAGGGGGAGGTCGGAGGAGGGGGCACCGCGGCGC CGGGTATATGACAAAGGACCCGGCTGCCCATCGCTCATGATCATGAAAAATATGGAGAACTGGAGGGCCG TGTGTAGGTGGCGGGGCCCCGAACGGACTAAGTGGCCAAGCCTTATTGAAAAGGGGGGGGGGTGGGTTCG ATAAAAC >SC323457 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCTGGCACTGGGAGAGGGCACCTATGCCACAGTCTTCAAAGGGCGCAGCAAACTGACGGAGAACCTTGTG GCCCTGATGGAGATCCGGCTGGAGCACGAGGAGGGAGCGCCCTGCACTGCCATCCGAGAGGTGTCTCTGC TGAAGAACCTGAAGCACGCCAATATTGTGACCCTGCATGACCTCATCCACACAGATCGGTCCCTCACCCT GGTGTTTGAGTACCTGGACAGTGACCTGAAGCAGTATCTGGACCA |
| Restriction Sites | Please inquire |
| ACCN | NM_002596 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002596.2, NP_002587.2 |
| RefSeq Size | 3157 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 5129 |
| UniProt ID | Q07002 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | May play a role in signal transduction cascades in terminally differentiated cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon found in the transcript. It should be noted that this start codon has a weak Kozak signal, and its conservation is restricted to primate species, squirrel, rock hyrax and armadillo. Two better conserved potential start codons with stronger Kozak signals are located two and five codons downstream, respectively. It is possible that leaky scanning by ribosomes would allow one of the downstream start codons to be used some of the time. There is no experimental evidence indicating which start codon is preferentially used in vivo. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202150 | CDK18 (Myc-DDK-tagged)-Human cyclin-dependent kinase 18 (CDK18), transcript variant 3 |
CNY 3,656.00 |
|
| RC202150L3 | Lenti ORF clone of Human cyclin-dependent kinase 18 (CDK18), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202150L4 | Lenti ORF clone of Human cyclin-dependent kinase 18 (CDK18), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG202150 | CDK18 (tGFP-tagged) - Human cyclin-dependent kinase 18 (CDK18), transcript variant 3 |
CNY 4,370.00 |
|
| SC324635 | CDK18 (untagged)-Human cyclin-dependent kinase 18 (CDK18), transcript variant 3 |
CNY 3,656.00 |
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