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TSSK2 (NM_053006) Human Untagged Clone
CAT＃: SC323471
TSSK2 (untagged)-Kinase deficient mutant (K41M) of Human testis-specific serine kinase 2 (TSSK2)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DGS-G; SPOGA2; STK22B; TSK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323471 sequence for NM_053006 edited (data generated by NextGen Sequencing)
ATGGACGATGCCACAGTCCTAAGGAAGAAGGGTTACATCGTAGGCATCAATCTTGGCAAG GGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGAGCGCCTCAAGTTCAATGTGGCTGTC AAGATCATCGACCGCAAGAAAACACCTACTGACTTTGTGGAGAGATTCCTTCCTCGGGAG ATGGACATCCTGGCAACTGTCAACCACGGCTCCATCATCAAGACTTACGAGATCTTTGAG ACCTCTGACGGACGGATCTACATCATCATGGAGCTTGGCGTCCAGGGCGACCTCCTCGAG TTCATCAAGTGCCAGGGAGCCCTGCATGAGGACGTGGCACGCAAGATGTTCCGACAGCTC TCCTCCGCCGTCAAGTACTGCCACGACCTGGACATCGTCCACCGGGACCTCAAGTGCGAG AACCTTCTCCTCGACAAGGACTTCAACATCAAGCTGTCTGACTTTGGCTTCTCCAAGCGC TGCCTGCGGGACAGCAATGGGCGCATCATCCTCAGCAAGACCTTCTGCGGGTCGGCAGCA TATGCAGCCCCCGAGGTGCTGCAGAGCATCCCCTACCAGCCCAAGGTGTATGACATCTGG AGCCTGGGCGTGATCCTGTACATCATGGTCTGCGGCTCCATGCCCTATGACGACTCCGAC ATCAGGAAGATGCTGCGTATCCAGAAGGAGCACCGTGTGGACTTCCCGCGCTCCAAGAAC CTGACCTGCGAGTGCAAGGACCTCATCTACCGCATGCTGCAGCCCGACGTCAGCCAGCGG CTCCACATCGATGAGATCCTCAGCCACTCGTGGCTGCAGCCCCCCAAGCCCAAAGCCACG TCTTCTGCCTCCTTCAAGAGGGAGGGGGAGGGCAAGTACCGCGCTGAGTGCAAACTGGAC ACCAAGACAGACTTGAGGCCCGACCACCGGCCCGACCACAAGCTTGGAGCCAAAACCCAG CACCGGCTGCTGGTGGTGCCCGAGAACGAGAACAGGATGGAGGACAGGCTGGCCGAGACC TCCAGGGCCAAAGACCATCACATCTCCGGAGCTGAGGTGGGGAAAGCAAGCACCTAG Clone variation with respect to NM_053006.4 911 g=>a >OriGene 5' read for mutant NM_053006 unedited
CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGA AGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGAGTCGAAGCCCAGCCCAGGGC AGCCCAGCCAGACGCCTCCGGTAGTGTAAATGAGGACAATGCCTGCTGGCCCACATGACGGGGGGATGTA GACGGCAGCGGCGCCAGTCGCTCCTGGCACCATGGACGATGCCACAGTCCTAAGGGAAGAGGGGTTACAT CGTAGGCATCAATCTTGGCAAGGGGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGACCGCCCTCAGGT TAAAGTGGGTCTGGTCAGGATCCATCGACCGCAGAAAAAGAACCGCCTCAGGTTCATGGTGGCTGTAAGG ATCTCCGACCCAAGGAAATGGACCCCCCAAAGTCAATGGTGCGGTGTAAGAATATCGCGCCCCAAAGAAA TTAGAGGCCTCAAGTTTCCAATTGGGCTTGCTATTAATTCCGAACCGAAGAGAAATGGAACCGGCCCTCG TTTACTAGTTGGGGCTGACAATTACACGACCGCGAAAAAATGTGCGCCCCCAGTTTCAAGTGGGGCTGAT GAGATCCACACCGAGAAAACACCTCCTGTGATTGTGAGAAATCTCTCCTCCGGAGAGGAGGACTCGGACA GTGTCACCGCGCTCATATGAATATAAGATATTGTAACACTGAGAGGAGTGTACTCATATGAGATGTGGCT GGGACACTCAGTGATTAATGCTCGAGCGTCTGATGAAGGCTGCCAAAGTAGTCAGCATCCTCTCCTCCGA ATAGTACGACTGAGCTGTCGCAGAAGCAATGTGAACATTCTCTGAGCAGAGCTACCCTGGTGAATGAAGT >SC323471 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGTAGCATCATCTTGGCAGGGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGAGCGCCTCAAGTTCAAT GTGTCTGTCATGATCATCGACCGCAAGAAAATGAGCGCCTCAAGTTCAATGTGGCTGTCATGATCATCGA CCGCAAGAAAATGAGCGCCTCAAGTTCAATGTGGCTGTCAAGATCATCGACCGCAAGAAAATGAGCGCCT CAAGTTCAATGTGGCTGTCATGATCATCGACCGCAAGAAAATGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_053006 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053006.3, NP_443732.2 |
| RefSeq Size | 1363 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 23617 |
| UniProt ID | Q96PF2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207232 | TSSK2 (Myc-DDK-tagged)-Human testis-specific serine kinase 2 (TSSK2) |
CNY 3,656.00 |
|
| RC207232L1 | Lenti ORF clone of Human testis-specific serine kinase 2 (TSSK2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207232L2 | Lenti ORF clone of Human testis-specific serine kinase 2 (TSSK2), mGFP tagged |
CNY 5,890.00 |
|
| RC207232L3 | Lenti ORF clone of Human testis-specific serine kinase 2 (TSSK2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207232L4 | Lenti ORF clone of Human testis-specific serine kinase 2 (TSSK2), mGFP tagged |
CNY 5,890.00 |
|
| RG207232 | TSSK2 (tGFP-tagged) - Human testis-specific serine kinase 2 (TSSK2) |
CNY 5,256.00 |
|
| SC125484 | TSSK2 (untagged)-Human testis-specific serine kinase 2 (TSSK2) |
CNY 3,656.00 |
