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PKC iota (PRKCI) (NM_002740) Human Untagged Clone
CAT＃: SC323672
PRKCI (untagged)-Kinase deficient mutant (K283M) of Human protein kinase C, iota (PRKCI)
CNY 4,376.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DXS1179E; nPKC-iota; PKCI |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323672 sequence for NM_002740 edited (data generated by NextGen Sequencing)
ATGCCGACCCAGAGGGACAGCAGCACCATGTCCCACACGGTCGCAGGCGGCGGCAGCGGG GACCATTCCCACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATCATGATAACACAT TTTGAACCTTCCATCTCCTTTGAGGGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTT GACAACGAACAGCTCTTCACCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACAGTA TCATCTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATGAGCTAAACAAGGATTCTGAA CTCTTGATTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGATGCCTTGTCCAGGAGAA GATAAATCCATCTACCGTAGAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGC CACACTTTCCAAGCCAAGCGTTTCAACAGGCGTGCTCACTGTGCCATCTGCACAGACCGA ATATGGGGACTTGGACGCCAAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAG AAGTGCCATAAACTCGTCACAATTGAATGTGGGCGGCATTCTTTGCCACAGGAACCAGTG ATGCCCATGGATCAGTCATCCATGCATTCTGACCATGCACAGACAGTAATTCCATATAAT CCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTGAAGAAAAAGAGGCAATGAACACCAGG GAAAGTGGCAAAGCTTCATCCAGTCTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATA GGAAGAGGAAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTAT GCAATGATRGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAG ACAGAGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCT TGCTTTCAGACAGAAAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCTA ATGTTTCATATGCAGCGACAAAGAAAACTTCCTGAAGAACATGCCAGATTTTACTCTGCA GAAATCAGTCTAGCATTAAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAA CTGGACAATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTACGGCATGTGT AAGGAAGGATTACGGCCAGGAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATT GCTCCTGAAATTTTAAGAGGAGAAGATTATGGTTTCAGTGTTGACTGGTGGGCTCTTGGA GTGCTCATGTTTGAGATGATGGCAGGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGAT AACCCTGACCAGAACACAGAGGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGC ATACCACGTTCTCTGTCTGTAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAATAAGGAC CCTAAGGAACGATTGGGTTGTCATCCTCAAACAGGATTTGCTGATATTCAGGGACACCCG TTCTTCCGAAATGTTGATTGGGATATGATGGAGCAAAAACAGGTGGTACCTCCCTTTAAA CCAAATATTTCTGGGGAATTTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACCT GTCCAGCTCACTCCAGATGACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAA GGTTTTGAGTATATCAATCCTCTTTTGATGTCTGCAGAAGAATGTGTCTGA Clone variation with respect to NM_002740.5 848 a=>t;849 a=>r >OriGene 5' read for mutant NM_002740 unedited
ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCTGTAGAGGCG GCGGCGCCTACGGGCAGTGGGAGGAGCCGCGCGGTTCCGGCTGCTCCGGCGAGGCGACCCTTGGGTCGGC GCTGCGGGCGAGGTGGGCAGGTAGGTGGGCGGACGGCCGCGGTTCTCCGGCAAGCGCAGGCGGCGGAGTC CCCCACGGCGCCCGAAGCGCCCCCCCCGCACCCCCGGGCCTCCAGCGTTGAGGCGGGGGGAGTGGAGGGA GGATGGCCGACCCAGAAGGGACAAGCCAGCCACCCATGTTCCCACAACGGTCGCAGGCGCGGCGGGCCGG CGGGGGAACCATTTCCCACCCAGTTTCCGGGGTGAAAGCCCTATAACCGGGGGGGAAAATAATGATAAAC CCATTTGGAAACCTCCCCTCCCCCTTTAGGGGGCTTTTGAAATAGGGGTCCAAAAAATTGTGTCCTTTTT GAACCCAAAAACAGCCTTTTCCCCTGTAAATGGGAATAAGTGAAGAGAGGGAACCCCTGGGTCCAAGTTC CCTCCATTTGAGATTTAAGAAAACCCTTTAAAACTTTTAAAACCTCAACGGGGTCTCGACACCTGGTACT ACGGGTCCCCTGTGGGCACCAAACAGCTTCTGGAGACCCTCTCCCCAGAAAAAAAATACCCCCCTCCCCC TAGGTGCGCCCCCCCCGTGAAAACATCTTTATTGGCGCAGGGGGCACTTTTTAGCACACCATTTTTTCAC GCGTCGCGCCGTGTGCTCTTCTCGACCACATTGTGGCGTCTCTGGCCCGAGTATAAGTCCTCCGGCACCT GTGTGTGAACAACCACCACTACACACACATATAGTGTGAGGCGACAGTGTCGCAGAGCACGCTAGGTCTT GCGATGTATGTCCTCCGATCTCGTACTACGTGACAGCAGGGAGGTATACCTG >SC323672 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ATGGATTGCTCGGGTATAGGAGAGGAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGT ATTTATGCAATGATGGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAGACAG AGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGA AAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCT |
| Restriction Sites | Please inquire |
| ACCN | NM_002740 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002740.4, NP_002731.3 |
| RefSeq Size | 4878 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 5584 |
| UniProt ID | P41743 |
| Domains | PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Endocytosis, Insulin signaling pathway, Tight junction |
| Gene Summary | This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205379 | PRKCI (Myc-DDK-tagged)-Human protein kinase C, iota (PRKCI) |
CNY 4,368.00 |
|
| RC205379L1 | Lenti ORF clone of Human protein kinase C, iota (PRKCI), Myc-DDK-tagged |
CNY 6,768.00 |
|
| RC205379L2 | Lenti ORF clone of Human protein kinase C, iota (PRKCI), mGFP tagged |
CNY 6,460.00 |
|
| RC205379L3 | Lenti ORF clone of Human protein kinase C, iota (PRKCI), Myc-DDK-tagged |
CNY 6,768.00 |
|
| RC205379L4 | Lenti ORF clone of Human protein kinase C, iota (PRKCI), mGFP tagged |
CNY 6,768.00 |
|
| RG205379 | PRKCI (tGFP-tagged) - Human protein kinase C, iota (PRKCI) |
CNY 5,968.00 |
|
| SC118455 | PRKCI (untagged)-Human protein kinase C, iota (PRKCI) |
CNY 4,376.00 |
|
| SC318027 | PRKCI (untagged)-Human protein kinase C, iota (PRKCI) |
CNY 7,220.00 |
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