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SGK1 (NM_005627) Human Untagged Clone
CAT＃: SC323700
SGK1 (untagged)-Kinase deficient mutant (K127M) of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SGK |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323700 sequence for NM_005627 edited (data generated by NextGen Sequencing)
ATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATG GTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTGAACGACTTTATTCAG AAGATTGCCAATAACTCCTATGCATGCAAACACCCTGAAGTTCAGTCCATCTTGAAGATC TCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCT CAGCAAATCAACCTTGGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTC TTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAA GAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAG AAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTG GGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCCTAGACTACATTAAT GGTGGAGAGTTGTTCTACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGT TTCTATGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCGTTTAT AGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGAC TTCGGACTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTTCTGTGGCACG CCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTTATGACAGGACTGTGGACTGG TGGTGCCTGGGAGCTGTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGA AACACAGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAAATATT ACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTC GGGGCCAAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTTAATTAACTGG GATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAAC GACCTACGGCACTTTGACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAG TCCCCTGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGC TTTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGA Clone variation with respect to NM_005627.3 >OriGene 5' read for mutant NM_005627 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTACACCGGCTCCT CCATGGTGCACATCCCTCCAGGGGAGCCAGACTTCGAGTCTTCCTTGTGTCAAACATGCCTGGGTGAACA TGCTTTCCAAAGAGGGGTTCTCCCTCAGGAGAACGAGTCATGTTCATGGGAAACTCAATCTGGGTGTGAA GTGAGAGAGCCATGTAATCATGCCAACATCCTGACCAAGCCCGATCCAAGAACCTTCTGGACTAATGATG ATCCCAGCTTTTCATGAAGCAGAGGAGGATGGGGTCTGAACGACTTTATTCAGAAGATTGCCAATAACTC TAATGCATGCAAACCACCCCTGGAGGTTCAGTCAATCTTGAGAATCTCCCCACCCTCAGGGACCCTGAGC TTAATGATTGCCACCCCTTCCCCTCAACCAGGTCTTTCTCAGCAAAATAACCCTGGCCCCTTGGTCAATT CTTAAGCCTAAACCAACCGGCCTTCAATTCCTGGAAATGTACGGAAAAGGGCGAGTTTTGGGAAAGGTTC CTCCTGCCAGACACAAAGCGCGAGAAATGTTTCTAGCGGTCTCGGGTTTACAGAGAAAACACACTCGTGA AAAAGAGAGGAGAGAGCATATTGTGTCAACCGATGTGCTCGTGTAAAAGTGAGAGCCCTTTCTCGTGGGG CCTTACTCTCTTCAGAAGTGTACAATGTACTTGGCCAGAGCACTATAGTGGAGAGATTGTCACACTCCAG GAGACTCTTCTGGACACGCCGTCTATGCTGGATACATGCTTGTCCTGATCGGACTGTAAGCTAACAGACT GGATCAGACGTGCCTAGAGCTGAGCTCG >SC323700 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ACYATGCTAACCATCTGACTTTCCTTCTTGAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGC AAGACACAAGGCAGAAGAAGTGTTCTATGCAGTCATGGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAA GAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCC TTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCCTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_005627 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005627.2, NP_005618.2 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 1296 bp |
| Locus ID | 6446 |
| UniProt ID | O00141 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009] Transcript Variant: This variant (1) represents the predominant transcript and encodes the shortest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201535 | SGK1 (Myc-DDK-tagged)-Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1 |
CNY 5,488.00 |
|
| RC201535L1 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201535L2 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201535L3 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201535L4 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG201535 | SGK1 (tGFP-tagged) - Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1 |
CNY 7,088.00 |
|
| SC116597 | SGK1 (untagged)-Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1 |
CNY 5,488.00 |
|
| SC322238 | SGK1 (untagged)-Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1 |
CNY 5,488.00 |
|
| SC323500 | SGK1 (untagged)-Kinase deficient mutant (K127M) of Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1 |
CNY 5,488.00 |
