KLF1 (NM_006563) Human Untagged Clone
CAT#: SC323869
KLF1 (untagged)-Human Kruppel-like factor 1 (erythroid) (KLF1)
CNY 5,488.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | EKLF; EKLF/KLF1 |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_006563.2
CTTGAGGCCCAGGGTGGGCACCAGCCAGCCATGGCCACAGCCGAGACCGCCTTGCCCTCC
ATCAGCACACTGACCGCCCTGGGCCCCTTCCCGGACACACAGGATGACTTCCTCAAGTGG TGGCGCTCCGAAGAGGCGCAGGACATGGGCCCGGGTCCTCCTGACCCCACGGAGCCGCCC CTCCACGTGAAGTCTGAGGACCAGCCCGGGGAGGAAGAGGACGATGAGAGGGGCGCGGAC GCCACCTGGGACCTGGATCTCCTCCTCACCAACTTCTCGGGCCCGGAGCCCGGTGGCGCG CCCCAGACCTGCGCTCTGGCGCCCAGCGAGGCCCCCGGGGCGCAATATCCGCCGCCGCCC GAGACTCTGGGCGCATATGCTGGCGGCCCGGGGCTGGTGGCTGGGCTTTTGGGTTCGGAG GATCACTCGGGTTGGGTGCGCCCTGCCCTGCGAGCCCGGGCTCCCGACGCCTTCGTGGGC CCAGCCCTGGCTCCAGCCCCGGCCCCCGAGCCCAAGGCGCTGGCGCTGCAACCGGTGTAC CCGGGGCCCGGCGCCGGCTCCTCGGGTGGCTACTTCCCGCGGACCGGGCTTTCAGTGCCT GCGGCGTCGGGCGCCCCCTACGGGCTACTGTCCGGGTACCCCGCGATGTACCCGGCGCCT CAGTACCAAGGGCACTTCCAGCTCTTCCGCGGGCTCCAGGGACCCGCGCCCGGTCCCGCC ACGTCCCCCTCCTTCCTGAGTTGTTTGGGACCCGGGACGGTGGGCACTGGACTCGGGGGG ACTGCAGAGGATCCAGGTGTGATAGCCGAGACCGCGCCATCCAAGCGAGGCCGACGTTCG TGGGCGCGCAAGAGGCAGGCAGCGCACACGTGCGCGCACCCGGGTTGCGGCAAGAGCTAC ACCAAGAGCTCCCACCTGAAGGCGCATCTGCGCACGCACACAGGGGAGAAGCCATACGCC TGCACGTGGGAAGGCTGCGGCTGGAGATTCGCGCGCTCGGACGAGCTGACCCGCCACTAC CGGAAACACACGGGGCAGCGCCCCTTCCGCTGCCAGCTCTGCCCACGTGCTTTTTCGCGC TCTGACCACCTGGCCTTGCACATGAAGCGCCACCTTTGAGCCCTGCCCTGGCACTTGGAC TCTCCTAGTGACTGGGGATGGGACAAGAAGCCTGTTTGGTGGTCTCTTCACACGGACGCG CGTGACACAATGCTGGGTGGTTTTCCCACGAATGGACCCTCTCCTGGACTCGCGTTCCCA AAGATCCACCCAAATATCAAACACGGACCCATAGACAGCCCTGGGGGAGCCTCTTACGGA AAATCCGACAAGCCTTCAGCCACAGGGAGCCACACAGAGATGTCCAAACTGTCGTGCAAA CCCAGTGAGACAGACCGCCAAATAAACGGACTCAGTGGACACTCAGACCAGCTCCCAGAT GGCCCTGGACAGCAGGAGAGGGTGTGGGATGAGGCTTCCCAGAGACCCTGGGTCTAGAAA GCGGCTCCTGAAGGTCCCTTATTGTGGCTGATATTAACTGTCAATGGTTATGGGTCCTAT AAAAATGCCCCTCCCAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | ECoRI-NOT |
ACCN | NM_006563 |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006563.2, NP_006554.1 |
RefSeq Size | 1618 bp |
RefSeq ORF | 1089 bp |
Locus ID | 10661 |
UniProt ID | Q13351 |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206452 | KLF1 (Myc-DDK-tagged)-Human Kruppel-like factor 1 (erythroid) (KLF1) |
CNY 3,656.00 |
|
RC206452L1 | Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC206452L2 | Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged |
CNY 5,890.00 |
|
RC206452L3 | Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC206452L4 | Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged |
CNY 5,890.00 |
|
RG206452 | KLF1 (tGFP-tagged) - Human Kruppel-like factor 1 (erythroid) (KLF1) |
CNY 5,256.00 |