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SF3B3 (NM_012426) Human Untagged Clone
CAT＃: SC323872
SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)
CNY 8,480.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RSE1; SAP130; SF3b130; STAF130 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_012426.3
TGTTGGAGTTGGTGGTGGCTTAAGTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACAC
CATCCTTCTCGCTGCAGGCTTTCTTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAG GTCTGGGTGGCTCAGGTTTCCTGCAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGC CACTGGCATCAGCTTTGCCATTCATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGT TGTTTCCCGTGGGAAGATCTTGGAGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACA TACCCTACTCACTGTGGAAGTATTCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGAC AGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGA ATACCAGCCATCTAAGAATATGTTTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGG ATGCCGTCGCATCGTTCCTGGCCAGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTAT GATTAGTGCCATTGAGAAACAGAAATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCG ACTTACCATTTCATCTCCCCTGGAAGCCCACAAAGCAAACACTTTAGTGTATCATGTAGT TGGAGTAGATGTCGGATTTGAAAATCCAATGTTTGCTTGTCTGGAAATGGATTATGAGGA AGCAGACAATGATCCAACAGGGGAAGCAGCAGCTAATACCCAGCAGACACTTACTTTCTA TGAGCTAGACCTTGGTTTAAATCATGTGGTCCGAAAATACAGTGAACCTTTGGAGGAACA CGGCAACTTCCTTATTACAGTTCCAGGAGGGTCAGATGGTCCAAGTGGAGTACTGATCTG CTCTGAAAACTATATTACTTACAAGAACTTTGGTGACCAGCCAGATATCCGCTGTCCAAT TCCCAGGAGGCGGAATGACCTGGATGACCCTGAAAGAGGAATGATTTTTGTCTGCTCTGC AACCCATAAAACCAAATCGATGTTCTTCTCTTTGGCTCAAACTGAGCAGGGAGATATCTT TAAGATCACTTTGGAGACAGATGAAGATATGGTTACTGAGATCCGGCTCAAATATTTTGA TACTGTACCCGTTGCTGCTGCCATGTGTGTGCTTAAAACAGGGTTCCTTTTTGTAGCATC AGAATTTGGAAACCATTACTTATATCAAATTGCACATCTTGGAGATGATGATGAAGAACC TGAGTTTTCATCAGCCATGCCTCTGGAAGAAGGAGACACATTCTTTTTTCAGCCAAGACC ACTTAAAAACCTTGTGCTGGTTGATGAGTTGGACAGCCTCTCTCCCATTCTGTTTTGCCA GATAGCTGATCTGGCCAATGAAGATACTCCACAGTTGTATGTGGCCTGTGGTAGGGGACC CCGATCATCTCTGAGAGTCCTAAGACATGGACTTGAGGTGTCAGAAATGGCTGTTTCTGA GCTACCTGGTAACCCCAACGCTGTCTGGACAGTGCGTCGACACATTGAAGATGAGTTTGA TGCCTACATTATTGTGTCTTTCGTGAATGCCACCCTAGTGTTGTCCATTGGAGAAACTGT AGAAGAAGTGACTGACTCTGGGTTCCTGGGGACCACCCCGACCTTGTCCTGCTCCTTATT AGGAGATGATGCCTTGGTGCAGGTCTATCCAGATGGCATTCGGCACATACGAGCAGACAA GAGAGTCAATGAGTGGAAGACCCCTGGAAAGAAAACAATTGTGAAGTGTGCAGTGAACCA GCGACAAGTGGTGATTGCCCTGACAGGAGGAGAGCTGGTCTATTTCGAGATGGATCCTTC AGGACAGCTGAATGAGTACACAGAACGGAAGGAGATGTCAGCAGATGTGGTGTGCATGAG TCTGGCCAATGTACCCCCTGGAGAGCAGCGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGA CAACACTGTCAGAATCATCTCCCTGGATCCCTCAGACTGTTTGCAACCTCTAAGCATGCA GGCTCTCCCAGCCCAGCCTGAGTCCTTGTGTATCGTGGAAATGGGTGGGACTGAGAAGCA GGATGAGCTGGGTGAGAGGGGCTCGATTGGCTTCCTATACCTGAATATTGGGCTACAGAA CGGTGTGCTGCTGAGGACTGTCTTGGACCCTGTCACTGGGGATTTGTCTGATACTCGCAC TCGGTACCTGGGGTCCCGTCCTGTGAAGCTCTTCCGAGTCCGAATGCAAGGCCAGGAGGC AGTATTGGCCATGTCAAGCCGCTCATGGTTGAGCTATTCTTACCAATCTCGCTTCCATCT CACCCCACTGTCTTACGAGACACTGGAATTTGCATCGGGTTTTGCCTCGGAACAGTGTCC CGAGGGCATTGTGGCCATCTCCACCAACACCCTACGGATTTTGGCATTAGAGAAGCTCGG TGCTGTCTTCAATCAAGTAGCCTTCCCACTGCAGTACACACCCAGGAAATTTGTCATCCA CCCTGAGAGTAACAACCTTATTATCATTGAAACGGACCACAATGCCTACACTGAGGCCAC GAAAGCTCAGAGAAAGCAGCAGATGGCAGAGGAAATGGTGGAAGCAGCAGGGGAGGATGA GCGGGAGCTGGCCGCAGAGATGGCAGCAGCATTCCTCAATGAAAACCTCCCTGAATCCAT CTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCTCTGTGATCCGAGTGATGAATCC CATTCAAGGGAACACACTGGACCTTGTCCAGCTGGAACAGAATGAGGCAGCTTTTAGTGT GGCTGTGTGCAGGTTTTCCAACACTGGTGAAGACTGGTATGTGCTGGTGGGTGTGGCCAA GGACCTGATACTAAACCCCCGATCTGTGGCAGGGGGCTTCGTCTATACTTACAAGCTTGT GAACAATGGGGAAAAACTGGAGTTTTTGCACAAGACTCCTGTGGAAGAGGTCCCTGCTGC TATTGCCCCATTCCAGGGGAGGGTGTTGATTGGTGTGGGGAAGCTGTTGCGTGTCTATGA CCTGGGAAAGAAGAAGTTACTCCGAAAATGTGAGAATAAGCATATTGCCAATTATATCTC TGGGATCCAGACTATCGGACATAGGGTAATTGTATCTGATGTCCAAGAAAGTTTCATCTG GGTTCGCTACAAGCGTAATGAAAACCAGCTTATCATCTTTGCTGATGATACCTACCCCCG ATGGGTCACTACAGCCAGCCTCCTGGACTATGACACTGTGGCTGGGGCAGACAAGTTTGG CAACATATGTGTGGTGAGGCTCCCACCTAACACCAATGATGAAGTAGATGAGGATCCTAC AGGAAACAAAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAGAAGGCAGAGGT GATCATGAACTACCATGTCGGGGAGACGGTGCTGTCCTTGCAGAAGACCACGCTGATCCC TGGAGGCTCAGAATCACTTGTCTATACCACCTTGTCTGGAGGAATTGGCATCCTTGTGCC ATTCACGTCCCATGAGGACCATGACTTCTTCCAGCATGTGGAAATGCACCTGCGGTCTGA ACATCCCCCTCTCTGTGGGCGGGACCACCTCAGCTTTCGCTCCTACTACTTCCCTGTGAA GAATGTGATTGATGGAGACCTCTGTGAGCAGTTCAATTCCATGGAACCCAACAAACAAAA GAACGTCTCTGAAGAACTGGACCGAACCCCACCCGAAGTGTCCAAGAAACTCGAGGATAT CCGGACCCGCTACGCCTTCTGAGCCCTCCTTTCCCGGTGGGGCTTGCCAGAGACTGTGTG TTTTGTTTCCCCCACCACCATCACTGCCACCTGGCTTCTGCCATGTGGCAGGAGGGTGAC TGGATAATTAAGACTGCATTATGAAAGTCAACAGCTCTTTCCCCTCAGCTCTTCTCCTGG AATGACTGGCTTCCCCTCAAATTGGCACTGAGATTTGCTACACTTCTCCCCACCTGGTAC ATGATACATGACCCCAGGTTCCAGTGTAGAACCTGAGTCCCCCATTCCCCAAAGCCATCC CTGCATTGATATGTCTTGACTCTCCTGTCTACTTTTGCACACACCCTTAATTTTTAATTG GTTTTCTTGTAAAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_012426 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012426.3, NP_036558.3 |
| RefSeq Size | 4357 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 23450 |
| UniProt ID | Q15393 |
| Domains | CPSF_A |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209509 | SF3B3 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,472.00 |
|
| RC209509L1 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 10,872.00 |
|
| RC209509L2 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RC209509L3 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC209509L4 | Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged |
CNY 14,060.00 |
|
| RG209509 | SF3B3 (tGFP-tagged) - Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 10,072.00 |
|
| SC126731 | SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) |
CNY 8,480.00 |
