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PPP3CB (NM_001142353) Human Untagged Clone
CAT＃: SC325163
PPP3CB (untagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALNA2; CALNB; CNA2; PP2Bbeta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325163 representing NM_001142353.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCCCCGGAGCCGGCCCGGGCTGCACCGCCCCCACCCCCGCCCCCGCCGCCCCCTCCCGGGGCT GACCGCGTCGTCAAAGCTGTCCCTTTCCCCCCAACACATCGCTTGACATCTGAAGAAGTATTTGATTTG GATGGGATACCCAGGGTTGATGTTCTGAAGAACCACTTGGTGAAAGAAGGTCGAGTAGATGAAGAAATT GCGCTTAGAATTATCAATGAGGGTGCTGCCATCCTTCGGAGAGAGAAAACCATGATAGAAGTAGAAGCT CCAATCACAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAACTTTTTGAAGTAGGAGGA TCACCTGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGTTATTTTAGTATAGAGTGT GTCTTATATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTCTTCTGAGAGGCAACCATGAA TGCAGACACCTTACTGAATATTTTACCTTTAAGCAGGAATGTAAAATTAAGTATTCGGAAAGAGTCTAT GAAGCTTGTATGGAAGCTTTTGATAGTTTGCCTCTTGCTGCACTTTTAAACCAACAGTTTCTTTGTGTT CATGGTGGACTTTCACCAGAAATACACACACTGGATGATATTAGGAGATTAGATAGATTCAAAGAGCCA CCTGCATTTGGACCAATGTGTGACTTGTTATGGTCCGATCCTTCTGAAGATTTTGGAAATGAAAAATCA CAGGAACATTTTAGTCACAATACAGTTCGAGGATGTTCTTATTTTTATAACTATCCAGCAGTGTGTGAA TTTTTGCAAAACAATAATTTGTTATCGATTATTAGAGCTCATGAAGCTCAAGATGCAGGCTATAGAATG TACAGAAAAAGTCAAACTACAGGGTTCCCTTCATTAATAACAATTTTTTCGGCACCTAATTACTTAGAT GTCTACAATAATAAAGCTGCTGTATTAAAGTATGAAAATAATGTGATGAATATTCGACAGTTTAACTGT TCTCCACATCCTTACTGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCTTTACCGTTTGTTGGAGAA AAAGTGACAGAAATGTTGGTAAATGTTCTGAGTATTTGCTCTGATGATGAACTAATGACTGAAGGTGAA GACCAGTTTGATGTAGGTTCAGCTGCAGCCCGGAAAGAAATCATAAGAAACAAAATTCGAGCAATTGGC AAGATGGCAAGAGTCTTCTCTGTTCTCAGGGAGGAGAGTGAAAGTGTGCTGACACTCAAGGGCCTGACT CCCACAGGGATGTTGCCTAGTGGAGTGTTAGCTGGAGGACGGCAGACCCTGCAAAGTGCCACAGTTGAG GCTATTGAGGCTGAAAAAGCAATACGAGGATTCTCTCCACCACATAGAATCTGCAGTTTTGAAGAGGCA AAGGGTTTGGATAGGATCAATGAGAGAATGCCACCTCGGAAAGATGCTGTACAGCAAGATGGTTTCAAT TCTCTGAACACCGCACATGCCACTGAGAACCACGGGACGGGCAACCATACTGCCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142353 |
| Insert Size | 1578 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142353.2 |
| RefSeq Size | 3540 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 5532 |
| UniProt ID | P16298 |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| MW | 59.1 kDa |
| Gene Summary | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:26794871). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227718 | PPP3CB (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 5,864.00 |
|
| RC227718L1 | Lenti-ORF clone of PPP3CB (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 8,264.00 |
|
| RC227718L2 | Lenti-ORF clone of PPP3CB (mGFP-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 5,890.00 |
|
| RC227718L3 | Lenti-ORF clone of PPP3CB (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 5,890.00 |
|
| RC227718L4 | Lenti-ORF clone of PPP3CB (mGFP-tagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 5,890.00 |
|
| RG227718 | PPP3CB (tGFP-tagged) - Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 1 |
CNY 7,464.00 |
