SUPT5H (NM_001130824) Human Untagged Clone

CAT#: SC325390

SUPT5H (untagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 3



  "NM_001130824" in other vectors (4)

CNY 10,450.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-SUPT5H Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms SPT5; SPT5H; Tat-CT1
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_001130824, the custom clone sequence may differ by one or more nucleotides
ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGAC
GGCGAGGAGGCCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAA
GAGCCTGAGGACGAAGAGGAGGAGGAAGAGGAGGAGGAATACGATGAGGAAGAGGAGGAA
GAAGATGATGACCGACCCCCCAAGAAACCCCGCCATGGAGGCTTCATTCTGGACGAGGCT
GATGTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGGATGGAGCAGAGGACATTCTA
GAGAAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGAAGATCGTTCT
GGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTAT
TACATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAG
CTCTCAGACGACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGG
ACTGTCAAATGTAAGATTGGGGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTC
ATTGCCTACCAGTTCACAGACACGCCCCTGCAGATCAAGTCAGTAGTGGCACCAGAGCAT
GTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCCACGTGAAGCAGGCCATTGAG
GGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCATCAAGGAGATG
ACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC
CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAG
AACACCATCTCCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATG
AGCTTGAAAGACTGGTTTGCCAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTT
GATGCTGAGAAGATCAGGTCCCTGGGGGGTGATGTTGCCTCTGATGGTGACTTCCTCATC
TTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTGTTCAAGAGCTTCGCCATGTCTGCT
GTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAGTTTGAGGACCAGCCA
GAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCACAACTTC
CAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATC
CTCAGCGTGGATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATG
TTGGAGTTCCCAGCCCAGGAACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTG
ATTGCTGGCCGATTCGAGGGCGACACAGGCCTCATTGTGCGGGTGGAGGAGAATTTCGTT
ATCCTGTTCTCTGACCTCACCATGCATGAGCTGAAGGTGCTCCCCCGGGACCTGCAGCTC
TGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAGCATGAATGGGGCGAGCTGGTG
CAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAGACCTTCCAGGTG
CTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAGGAC
AACCGCTTTGCTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTT
AAGGTCATTGATGGCCCCCACTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGC
TTCGCCTTCCTACATTGCAAGAAACTGGTGGAGAACGGGGGCATGTTTGTCTGCAAGACC
CGCCACCTGGTGCTGGCTGGGGGCTCAAAGCCCCGTGATGTGACCAACTTCACCGTGGGT
GGCTTTGCGCCTATGAGTCCCCGGATCAGCAGCCCCATGCACCCCAGTGCTGGAGGTCAG
CGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATGAGCAGGGGCCGGGGCCGG
AGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTACAAAGGCTAC
ATCGGTGTGGTGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGC
CAGACCATCTCTGTGGACCGTCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGC
ATGACCTCGACCTATGGGAGGACGCCCATGTATGGCTCCCAGACGCCCATGTATGGCTCT
GGCTCCCGAACACCCATGTACGGCTCACAGACACCCCTCCAGGATGGTAGCCGCACCCCA
CACTACGGCTCACAGACGCCCCTGCATGATGGCAGCCGCACTCCTGCCCAGAGTGGGGCC
TGGGACCCCAACAACCCCAACACGCCGTCACGGGCTGAGGAAGAATATGAGTATGCTTTC
GATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCCAATCCCCAAACACCT
GGCTACCCAGACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACGCCAGGG
ACGCCGGCCATGTACAACACAGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGT
TCCTACCAGCCCAGCCCCAGCCCCCAGAGCTACCACCAGGTGGCGCCAAGCCCAGCAGGC
TACCAGAATACCCACTCCCCAGCCAGCTACCACCCTACACCGTCGCCCATGGCCTATCAG
GCTAGCCCCAGCCCGAGCCCCGTTGGCTACAGTCCTATGACACCTGGAGCTCCCTCCCCT
GGTGGCTACAACCCACACACGCCAGGCTCAGGCATCGAGCAGAACTCCAGCGACTGGGTA
ACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTGGATACACAGGTGGTGGGACAG
ACAGGTGTCATCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTGAAGGACAGTGAG
AAGGTTGTCAGCATTTCCAGTGAGCACCTGGAGCCTATCACCCCCACCAAGAACAACAAG
GTGAAAGTGATCCTGGGCGAGGATCGGGAAGCCACGGGCGTCCTACTGAGCATTGATGGT
GAGGATGGCATTGTCCGTATGGACCTTGATGAGCAGCTCAAGATCCTCAACCTCCGCTTC
CTGGGGAAGCTCCTGGAAGCC
Restriction Sites Please inquire     
ACCN NM_001130824
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001130824.1, NP_001124296.1
RefSeq Size 3659 bp
RefSeq ORF 3264 bp
Locus ID 6829
UniProt ID O00267
Protein Families Transcription Factors
Gene Summary Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 5 all encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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