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SPHKAP (NM_001142644) Human Untagged Clone
CAT＃: SC325420
SPHKAP (untagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1
CNY 26,790.00
货期*
4周
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SKIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325420 representing NM_001142644.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAA CCGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAG GTTCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAA ATTGGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAG GATGAATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATC AGTTCCATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAAT CTCCAGGCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGA GGGAACAGACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTG GTGCAGGAGCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTA TCTTCAATCGAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCT AGGAACGATTATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACC CAGGTGGAATGGAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATAT CCCACACCATTGATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTA GATCCCTCAGCCAAGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTAT TATCATTCAGAAGCTTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTAT TTCTCCATGATGGATAAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCA GGAGACCATGAAGACACAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTAT GCTACAAATTTAGCAGAATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTA CCCCAGGAATCTGCAGTCAGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGAT ACAGTGGTTTCTCGGTCATGGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGAT GCTGCCCCACAGCCAGGCATCTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTC TCTGGAGAGAACTCCAGCAGACAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTG ATTGGAACTATTTCCAGTCCACAGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCC CCAGGGAGCAGTGGTGCACTGCAAACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTAC TCCTTTCCATCTGCTTTGTGTGGCATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAA AGAGAAGAGGTGACATGCTCAGTGGCTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCC ATGCCCCCACTTTGTGGTTTAGCAAGCATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAG GAGGCTGCTCTGGTTTTAACAAGGCCTAATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAAC AGGAGAATCATGGAAACTGCTTCAAAGTCTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTG GCACATACCCTGTCCAATGTTATCCTGAGGCATTCCATTGATGAAGTTCACCACAAAAATATGATAATC GACCCCAATGACAACAGGCATTCATCTGAAATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTT TTAGATGTGATATGCTTCACGTTCAAGAAGATGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTC CTTTCTAAGGAGACCATCAGAAGGAGGGAGACAGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGT CAAGCTTGGACAAAAGCCACTGAATCCTCCAGCAGCTCTCCACTTAGCAATTCACACAACACGAGTCTT GTCATCAACAATCTTGTGGATGGCATGTATTCAAAACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTC AAGAACCCCACGCTGCAGTCACAATTATCACGTAGTCACAGAGTGCCCGATTCTTCAACTGCTACAACA TCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGAGAGGATACAAAAAGCCCTCATCACAGTGAGAAT GAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAACGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAG GCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTACAACTGTGCCACATCTCGCATCAACGAAGTT CAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTTCCTGCTCAATCCACGCTTCAAACAAAGCAT CCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAGCAGACACGGTCGTCTCCATGGCAACTGAA ATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACCCTGGTTTTGTGCATGGAAAAGAGGGAGT GAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAGAGGAAGAAAGAGAGCCAGGGGAGCGGG ACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCAAGAGGAAGACGGACGAGCACCCTGAG CTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAACCTTGAAGATGTCCCAGATTCTGTC AATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACGGAGTTCTCTATGGTGGACGGCATG TGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACAGGTGGAGCCGGCTGAAGGCCTCC AGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGTCAACAGCCTGGGCTTAATGAGC ACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCGAGCTGTGAGAGCATCACCGAT GAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGATTTGAGTTACTGCTGGATTAC TATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGCGTGCCGGAAAAGTGACCAC CTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATCACTGAGGAGTTCTACAGG TACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGACGGAGCAGCCAGGATTGG ACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTCGTCCATGCCAGACAGC AGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTAGATGGCTTTGCTCAG AACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCGGTCTCTGCAAATCT GACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCATGAAACGTGGGCT AGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAAGCTGACACTGAG CCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGAGCAGGATGTGC AGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTACCGAATGTAAA CAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGATTCTAAAAAA GAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGAGGGAAGTG CCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCTTTCCAAA AGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGAGGTGGA GACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGGCTGAA GCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCACAGGC AGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGTGAG CGATCCATGAGCAATGGCAACAGTAGTGCCACTAGCAGTCTTGGCATTATGGATCTGGACATTTATCAG GAAAGCATGCCATCTTCTCCCATGATTAATGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCA GAAAGCACAGAGGCACCTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTG ATCAACTTTGACCTGGAGCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCC TCTGAACTGGGGATTCCCACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGAT GTCGTGCAGCTGGTTCATCGGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGC AAAATGCATGAGGAGCAGAAGGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142644 |
| Insert Size | 5103 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142644.1 |
| RefSeq Size | 6913 bp |
| RefSeq ORF | 5103 bp |
| Locus ID | 80309 |
| UniProt ID | Q2M3C7 |
| Protein Families | Druggable Genome |
| MW | 186.5 kDa |
| Gene Summary | Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227959 | SPHKAP (Myc-DDK-tagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1 |
CNY 18,280.00 |
|
| RC227959L1 | Lenti ORF clone of Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, Myc-DDK-tagged |
CNY 20,680.00 |
|
| RC227959L3 | Lenti ORF clone of Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, Myc-DDK-tagged |
CNY 15,680.00 |
|
| RG227959 | SPHKAP (tGFP-tagged) - Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1 |
CNY 15,200.00 |
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